Startseite Long noncoding RNA Linc01296 promotes hepatocellular carcinoma development through regulation of the miR-26a/PTEN axis
Artikel
Lizenziert
Nicht lizenziert Erfordert eine Authentifizierung

Long noncoding RNA Linc01296 promotes hepatocellular carcinoma development through regulation of the miR-26a/PTEN axis

  • Libin Zhang , Jing Hu , Menghui Hao EMAIL logo und Liang Bu EMAIL logo
Veröffentlicht/Copyright: 18. Juli 2019
Biological Chemistry
Aus der Zeitschrift Biological Chemistry Band 401 Heft 3

Abstract

Long noncoding RNA 01296 (Lnc01296) is dysregulated in malignant tumors. However, the detailed effect of Linc01296 on hepatocellular carcinoma (HCC) remains largely unknown. In this study, we identified the biological role of Linc01296 in HCC. The levels of Linc01296 in HCC tissues and a panel of cell lines were assessed by quantitative real-time polymerase chain reaction (qRT-PCR). The effects of Linc01296 on HCC progression were explored using a Cell Counting Kit-8 (CCK-8), flow cytometry, migration and Transwell invasion assays. The interactions among Linc01296, miR-26a and PTEN were determined using luciferase, RNA immunoprecipitation (RIP) and Western blot assays. Tumor xenograft models were utilized to confirm the in vivo functional roles of Linc01296 in HCC development. Linc01296 expression was increased in both HCC tissue samples and cell lines. Knockdown of Linc01296 suppressed HCC cell processes, such as proliferation, migration and invasion, and enhanced apoptosis in vitro; these effects were reversed by a miR-26a mimic or PTEN overexpression. Furthermore, knockdown of Linc01296 suppressed HCC growth in vivo. These findings indicated that Linc01296 is involved in HCC progression via regulating miR-26a/PTEN.

Keywords: hepatocellular carcinoma; Linc01296; miR-26a; PTEN

Funding source: Huimin Special Science and Technology

Award Identifier / Grant number: 2017ZF026

Funding source: scientific research projects in Yunnan Province

Award Identifier / Grant number: 2017NS256

Funding statement: This project was supported by a grant from Huimin Special Science and Technology (No. 2017ZF026) and the medical and health units of research institutions for scientific research projects in Yunnan Province (No. 2017NS256).

  1. Conflict of interest statement: The authors declare no conflicts of interest.

References

Chen, S. and Xia, X. (2019). Long noncoding RNA NEAT1 suppresses sorafenib sensitivity of hepatocellular carcinoma cells via regulating miR-335-c-Met. J. Cell. Physiol. doi: https://doi.org/10.1002/jcp.2756710.1002/jcp.27567Suche in Google Scholar PubMed

Coronel-Hernández, J., López-Urrutia, E., Contreras-Romero, C., Delgado-Waldo, I., Figueroa-González, G., Campos-Parra, A.D., Salgado-García, R., Martínez-Gutierrez, A., Rodríguez-Morales, M., Jacobo-Herrera, N., et al. (2019). Cell migration and proliferation are regulated by miR-26a in colorectal cancer via the PTEN-AKT axis. Cancer Cell Int. 19, 80.10.1186/s12935-019-0802-5Suche in Google Scholar PubMed PubMed Central

Ding, K., Wu, Z., Wang, N., Wang, X., Wang, Y., Qian, P., Meng, G., and Tan, S. (2017). MiR-26a performs converse roles in proliferation and metastasis of different gastric cancer cells via regulating of PTEN expression. Pathol. Res. Pract. 213, 467–475.10.1016/j.prp.2017.01.026Suche in Google Scholar PubMed

Floudas, C.S., Brar, G., and Greten, T.F. (2019). Immunotherapy: current status and future perspectives. Dig. Dis. Sci. 64, 1030–1040.10.1007/s10620-019-05516-7Suche in Google Scholar PubMed

Fu, X., Wen, H., Jing, L., Yang, Y., Wang, W., Liang, X., Nan, K., Yao, Y., and Tian, T. (2017). MicroRNA-155-5p promotes hepatocellular carcinoma progression by suppressing PTEN through the PI3K/Akt pathway. Cancer Sci. 108, 620–631.10.1111/cas.13177Suche in Google Scholar PubMed PubMed Central

Gao, X.M., Zhu, Y., Li, J.H., Wang, X.Y., Zhang, X.F., Yi, C.H., and Yang, X. (2018). microRNA-26a induces a mitochondrial apoptosis mediated by p53 through targeting to inhibit Mcl1 in human hepatocellular carcinoma. Onco Targets Ther. 11, 2227–2239.10.2147/OTT.S160895Suche in Google Scholar PubMed PubMed Central

Gnoni, A., Santini, D., Scartozzi, M., Russo, A., Licchetta, A., Palmieri, V., Lupo, L., Faloppi, L., Palasciano, G., Memeo, V., et al. (2015). Hepatocellular carcinoma treatment over sorafenib: epigenetics, microRNAs and microenvironment. Is there a light at the end of the tunnel? Expert Opin Ther. Targets 19, 1623–1635.10.1517/14728222.2015.1071354Suche in Google Scholar PubMed

He, J., Mu, M., Luo, Y., Wang, H., Ma, H., Guo, S., Fang, Q., Qian, Z., Lu, H., and Song, C. (2019). MicroRNA-20b promotes proliferation of H22 hepatocellular carcinoma cells by targeting PTEN. Oncol. Lett. 17, 2931–2936.10.3892/ol.2019.9925Suche in Google Scholar PubMed PubMed Central

Hou, Z., Xu, X., Fu, X., Tao, S., Zhou, J., Liu, S., and Tan, D. (2017). HBx-related long non-coding RNA MALAT1 promotes cell metastasis via up-regulating LTBP3 in hepatocellular carcinoma. Am. J. Cancer Res. 7, 845–856.Suche in Google Scholar

Hu, X., Duan, L., Liu, H., and Zhang, L. (2019). Long noncoding RNA LINC01296 induces non-small cell lung cancer growth and progression through sponging miR-5095. Am. J. Transl. Res. 11, 895–903.Suche in Google Scholar

Ierardi, A.M., Angileri, S.A., Patella, F., Panella, S., Lucchina, N., Petre, E.N., Pinto, A., Franceschelli, G., Carrafiello, G., Cornalba, G., et al. (2017). The role of interventional radiology in the treatment of intrahepatic cholangiocarcinoma. Med. Oncol. 34, 11.10.1007/s12032-016-0866-1Suche in Google Scholar PubMed

Ji, D., Jiang, C., Zhang, L., Liang, N., Jiang, T., Yang, B., and Liang, H. (2018). LncRNA CRNDE promotes hepatocellular carcinoma cell proliferation, invasion, and migration through regulating miR-203/ BCAT1 axis. J. Cell. Physiol. 234, 6548–6560.10.1002/jcp.27396Suche in Google Scholar PubMed

Jin, L., He, Y., Tang, S., and Huang, S. (2018). LncRNA GHET1 predicts poor prognosis in hepatocellular carcinoma and promotes cell proliferation by silencing KLF2. J. Cell. Physiol. 233, 4726–4734.10.1002/jcp.26257Suche in Google Scholar PubMed

Kang, T.W., Lim, H.K., and Cha, D.I. (2018). Percutaneous ablation for perivascular hepatocellular carcinoma: Refining the current status based on emerging evidence and future perspectives. World J. Gastroenterol. 24, 5331–5337.10.3748/wjg.v24.i47.5331Suche in Google Scholar PubMed PubMed Central

Li, Y., Ren, M., Zhao, Y., Lu, X., Wang, M., Hu, J., Lu, G., and He, S. (2017). MicroRNA-26a inhibits proliferation and metastasis of human hepatocellular carcinoma by regulating DNMT3B-MEG3 axis. Oncol. Rep. 37, 3527–3535.10.3892/or.2017.5579Suche in Google Scholar PubMed

Li, Y., Guo, D., Zhao, Y., Ren, M., Lu, G., Wang, Y., Zhang, J., Mi, C., He, S., and Lu, X. (2018). Long non-coding RNA SNHG5 promotes human hepatocellular carcinoma progression by regulating miR-26a-5p/GSK3β signal pathway. Cell Death Dis. 9, 888.10.1038/s41419-018-0882-5Suche in Google Scholar PubMed PubMed Central

Li, X., Wang, Q., Rui, Y., Zhang, C., Wang, W., Gu, J., Tang, J., and Ding, Y. (2019). HOXC13-AS promotes breast cancer cell growth through regulating miR-497-5p/PTEN axis. J. Cell. Physiol. doi: 10.1002/jcp.28800.10.1002/jcp.28800Suche in Google Scholar PubMed

Liu, B., Wu, X., Liu, B., Wang, C., Liu, Y., Zhou, Q., and Xu, K. (2012). MiR-26a enhances metastasis potential of lung cancer cells via AKT pathway by targeting PTEN. Biochim. Biophys. Acta 1822, 1692–1704.10.1016/j.bbadis.2012.07.019Suche in Google Scholar PubMed

Liu, B., Pan, S., Xiao, Y., Liu, Q., Xu, J., and Jia, L. (2018). LINC01296/miR-26a/GALNT3 axis contributes to colorectal cancer progression by regulating O-glycosylated MUC1 via PI3K/AKT pathway. J. Exp. Clin. Cancer Res. 37, 316.10.1186/s13046-018-0994-xSuche in Google Scholar PubMed PubMed Central

Ma, M., Xu, H., Liu, G., Wu, J., Li, C., Wang, X., Zhang, S., Xu, H., Ju, S., and Cheng, W. (2019). MITA1, a novel energy stress-inducible lncRNA, promotes hepatocellular carcinoma metastasis. Hepatology 70, 215–230.10.1002/hep.30602Suche in Google Scholar

Qin, Q.H., Yin, Z.Q., Li, Y., Wang, B.G., and Zhang, M.F. (2018). Long intergenic noncoding RNA 01296 aggravates gastric cancer cells progress through miR-122/MMP-9. Biomed. Pharmacother. 97, 450–457.10.1016/j.biopha.2017.10.066Suche in Google Scholar PubMed

Sia, D., Villanueva, A., Friedman, S.L., and Llovet, J.M. (2017). Liver cancer cell of origin, molecular class, and effects on patient prognosis. Gastroenterology 152, 745–761.10.1053/j.gastro.2016.11.048Suche in Google Scholar PubMed

Tu, W., Yang, Y., Song, Y., and Zhu, W. (2019). Hepatitis B virus x protein accelerated the proliferation of hepatocellular carcinoma cell through lncRNA SNHG20/PTEN pathway. J. Biochem. 165, 423–431.10.1093/jb/mvy120Suche in Google Scholar PubMed

Wang, J. and Sun, G. (2017). FOXO1-MALAT1-miR-26a-5p feedback loop mediates proliferation and migration in osteosarcoma cells. Oncol. Res. 25, 1517–1527.10.3727/096504017X14859934460780Suche in Google Scholar PubMed PubMed Central

Wang, H., Huo, X., Yang, X.R., He, J., Cheng, L., Wang, N., Deng, X., Jin, H., Wang, N., Wang, C., et al. (2017). STAT3-mediated upregulation of lncRNA HOXD-AS1 as a ceRNA facilitates liver cancer metastasis by regulating SOX4. Mol. Cancer 16, 136.10.1186/s12943-017-0680-1Suche in Google Scholar PubMed PubMed Central

Wang, L., Meng, D., Wang, Y., and Hu, J. (2018a). Long non-coding RNA LINC01296 promotes esophageal squamous cell carcinoma cell proliferation and invasion by epigenetic suppression of KLF2. Am. J. Cancer Res. 8, 2020–2029.Suche in Google Scholar

Wang, K., Zhang, M., Wang, C., and Ning, X. (2018b). Long noncoding RNA LINC01296 harbors miR-21a to regulate colon carcinoma proliferation and invasion. Oncol. Res. 27, 541–549.10.3727/096504018X15234931503876Suche in Google Scholar PubMed PubMed Central

Wang, Y., Cao, L., Wang, Q., Huang, J., and Xu, S. (2019a). LncRNA FOXD2-AS1 induces chondrocyte proliferation through sponging miR-27a-3p in osteoarthritis. Artif. Cells Nanomed. Biotechnol. 47, 1241–1247.10.1080/21691401.2019.1596940Suche in Google Scholar PubMed

Wang, X., Lyu, J., Ji, A., Zhang, Q., and Liao, G. (2019b). Jarid2 enhances the progression of bladder cancer through regulating PTEN/AKT signaling. Life Sci. 230, 162–168.10.1016/j.lfs.2019.05.053Suche in Google Scholar PubMed

Wu, D.M., Han, X.R., Wen, X., Wang, S., Wang, Y.J., Shen, M., Fan, S.H., Zhuang, J., Zhang, Z.F., Shan, Q., et al. (2018). Long non-coding RNA LINC01260 inhibits the proliferation, migration and invasion of spinal cord glioma cells by targeting CARD11 via the NF-κB signaling pathway. Cell. Physiol. Biochem. 48, 1563–1578.10.1159/000492279Suche in Google Scholar PubMed

Wu, Y., Zhou, Y., Huan, L., Xu, L., Shen, M., Huang, S., and Liang, L. (2019). LncRNA MIR22HG inhibits growth, migration and invasion through regulating the miR-10a-5p/NCOR2 axis in hepatocellular carcinoma cells. Cancer Sci. 110, 973–984.10.1111/cas.13950Suche in Google Scholar PubMed PubMed Central

Xu, L., Wei, B., Hui, H., Sun, Y., Liu, Y., Yu, X., and Dai, J. (2018). Positive feedback loop of lncRNA LINC01296/miR-598/Twist1 promotes non-small cell lung cancer tumorigenesis. J. Cell. Physiol. 234, 4563–4571.10.1002/jcp.27235Suche in Google Scholar PubMed

Yang, J., Li, C., Li, H., and E, C. (2019). LncRNA CACNA1G-AS1 facilitates hepatocellular carcinoma progression through the miR-2392/C1orf61 pathway. J. Cell. Physiol. 234, 18415–18422.10.1002/jcp.28477Suche in Google Scholar PubMed

Yu, W., Li, D., Ding, X., Sun, Y., Liu, Y., Cong, J., Yang, J., Sun, J., Ning, X., Wang, H., et al. (2019). LINC00702 suppresses proliferation and invasion in non-small cell lung cancer through regulating miR-510/PTEN axis. Aging 11, 1471–1485.10.18632/aging.101846Suche in Google Scholar PubMed PubMed Central

Yuan, Q., Zhang, Y., Feng, L., and Jiang, Y. (2019). Upregulated long noncoding RNA LINC01296 indicates a dismal prognosis for pancreatic ductal adenocarcinoma and promotes cell metastatic properties by affecting EMT. J. Cell. Biochem. 120, 552–561.10.1002/jcb.27411Suche in Google Scholar PubMed

Zeng, X., Hu, Z., Ke, X., Tang, H., Wu, B., Wei, X., and Liu, Z. (2017). Long noncoding RNA DLX6-AS1 promotes renal cell carcinoma progression via miR-26a/PTEN axis. Cell Cycle 16, 2212–2219.10.1080/15384101.2017.1361072Suche in Google Scholar PubMed PubMed Central

Zhang, D., Li, H., Xie, J., Jiang, D., Cao, L., Yang, X., Xue, P., and Jiang, X. (2018a). Long noncoding RNA LINC01296 promotes tumor growth and progression by sponging miR-5095 in human cholangiocarcinoma. Int. J. Oncol. 52, 1777–1786.10.3892/ijo.2018.4362Suche in Google Scholar PubMed PubMed Central

Zhang, L., Liu, X., Liu, J., Ma, X., Zhou, Z., Song, Y., and Cao, B. (2018b). miR-26a promoted endometrial epithelium cells (EECs) proliferation and induced stromal cells (ESCs) apoptosis via the PTEN-PI3K/AKT pathway in dairy goats. J. Cell. Physiol. 233, 4688–4706.10.1002/jcp.26252Suche in Google Scholar PubMed

Zhang, L., Liu, S.K., Song, L., and Yao, H.R. (2019a). SP1-induced up-regulation of lncRNA LUCAT1 promotes proliferation, migration and invasion of cervical cancer by sponging miR-181a. Artif. Cells Nanomed. Biotechnol. 47, 556–564.10.1080/21691401.2019.1575840Suche in Google Scholar PubMed

Zhang, H., Wang, S., Cacalano, N., Zhu, H., Liu, Q., Xie, M., Kamrava, M., Konecny, G., and Jin, S. (2019b). Oncogenic Y68 frame shift mutation of PTEN represents a mechanism of docetaxel resistance in endometrial cancer cell lines. Sci. Rep. 9, 2111.10.1038/s41598-019-38585-9Suche in Google Scholar PubMed PubMed Central

Zhao, D., Wang, S., Chu, X., and Han, D. (2019). LncRNA HIF2PUT inhibited osteosarcoma stem cells proliferation, migration and invasion by regulating HIF2 expression. Artif. Cells Nanomed. Biotechnol. 47, 1342–1348.10.1080/21691401.2019.1596934Suche in Google Scholar PubMed

Zhou, X., Zhu, H., and Lu, J. (2015). PTEN and hTERT gene expression and the correlation with human hepatocellular carcinoma. Pathol. Res. Pract. 211, 316–319.10.1016/j.prp.2014.11.016Suche in Google Scholar PubMed

Zhuang, C., Wang, P., Huang, D., Xu, L., Wang, X., Wang, L., and Hu, L. (2016). A double-negative feedback loop between EZH2 and miR-26a regulates tumor cell growth in hepatocellular carcinoma. Int. J. Oncol. 48, 1195–1204.10.3892/ijo.2016.3336Suche in Google Scholar PubMed

Received: 2019-04-21
Accepted: 2019-07-09
Published Online: 2019-07-18
Published in Print: 2020-02-25

©2020 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

  1. Frontmatter
  2. Reviews
  3. Genetic, epigenetic and biochemical regulation of succinate dehydrogenase function
  4. Transport mechanism of Mycobacterium tuberculosis MmpL/S family proteins and implications in pharmaceutical targeting
  5. Research Articles/Short Communications
  6. Genes and Nucleic Acids
  7. LncRNA MALAT1 inhibits hypoxia/reoxygenation-induced human umbilical vein endothelial cell injury via targeting the microRNA-320a/RAC1 axis
  8. Protein Structure and Function
  9. Identification of new probe substrates for human CYP20A1
  10. Molecular Medicine
  11. Exosomal MALAT1 derived from ox-LDL-treated endothelial cells induce neutrophil extracellular traps to aggravate atherosclerosis
  12. Cell Biology and Signaling
  13. Derlin-1 functions as a growth promoter in breast cancer
  14. Alterations in rat adipose tissue transcriptome and proteome in response to prolonged fasting
  15. Long noncoding RNA Linc01296 promotes hepatocellular carcinoma development through regulation of the miR-26a/PTEN axis
  16. Tumor-suppressive activity of sTRAIL on circulating CD44+ cells in patients with non-small cell lung cancer
https://doi.org/10.1515/hsz-2019-0231
Schlagwörter für diesen Artikel
hepatocellular carcinoma; Linc01296; miR-26a; PTEN

Artikel in diesem Heft

  1. Frontmatter
  2. Reviews
  3. Genetic, epigenetic and biochemical regulation of succinate dehydrogenase function
  4. Transport mechanism of Mycobacterium tuberculosis MmpL/S family proteins and implications in pharmaceutical targeting
  5. Research Articles/Short Communications
  6. Genes and Nucleic Acids
  7. LncRNA MALAT1 inhibits hypoxia/reoxygenation-induced human umbilical vein endothelial cell injury via targeting the microRNA-320a/RAC1 axis
  8. Protein Structure and Function
  9. Identification of new probe substrates for human CYP20A1
  10. Molecular Medicine
  11. Exosomal MALAT1 derived from ox-LDL-treated endothelial cells induce neutrophil extracellular traps to aggravate atherosclerosis
  12. Cell Biology and Signaling
  13. Derlin-1 functions as a growth promoter in breast cancer
  14. Alterations in rat adipose tissue transcriptome and proteome in response to prolonged fasting
  15. Long noncoding RNA Linc01296 promotes hepatocellular carcinoma development through regulation of the miR-26a/PTEN axis
  16. Tumor-suppressive activity of sTRAIL on circulating CD44+ cells in patients with non-small cell lung cancer
Jetzt anmelden und 20% sparen
Institutioneller Zugang
Wie funktioniert Authentifizierung?
Haben Sie eine Idee, wie wir unsere Website verbessern können?
Bitte schreiben Sie uns.
© 2025 De Gruyter Brill
Heruntergeladen am 25.9.2025 von https://www.degruyterbrill.com/document/doi/10.1515/hsz-2019-0231/html
Button zum nach oben scrollen