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Dynamic characteristics of the mitochondrial genome in SCNT pigs

  • Tao Yin , Jikun Wang , Hai Xiang , Carl A. Pinkert EMAIL logo , Qiuyan Li EMAIL logo and Xingbo Zhao EMAIL logo
Published/Copyright: November 13, 2018

Abstract

Most animals generated by somatic cell nuclear transfer (SCNT) are heteroplasmic; inheriting mitochondrial genetics from both donor cells and recipient oocytes. However, the mitochondrial genome and functional mitochondrial gene expression in SCNT animals are rarely studied. Here, we report the production of SCNT pigs to study introduction, segregation, persistence and heritability of mitochondrial DNA transfer during the SCNT process. Porcine embryonic fibroblast cells from male and female Xiang pigs were transferred into enucleated oocytes from Yorkshire or Landrace pigs. Ear biopsies and blood samples from SCNT-derived pigs were analyzed to characterize the mitochondrial genome haplotypes and the degree of mtDNA heteroplasmy. Presence of nuclear donor mtDNA was less than 5% or undetectable in ear biopsies and blood samples in the majority of SCNT-derived pigs. Yet, nuclear donor mtDNA abundance in 14 tissues in F0 boars was as high as 95%. Additionally, mtDNA haplotypes influenced mitochondrial respiration capacity in F0 fibroblast cells. Our results indicate that the haplotypes of recipient oocyte mtDNA can influence mitochondrial function. This leads us to hypothesize that subtle developmental influences from SCNT-derived heteroplasmy can be targeted when using donor and recipient mitochondrial populations from breeds of swine with limited evolutionary divergence.

Acknowledgment

This work was supported by the National Key Basic Research Program of China (Grant number: 2014CB138500).

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Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/hsz-2018-0273).


Received: 2018-06-04
Accepted: 2018-10-07
Published Online: 2018-11-13
Published in Print: 2019-05-27

©2019 Walter de Gruyter GmbH, Berlin/Boston

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