Startseite Pulmonary infection of cystic fibrosis mice with Staphylococcus aureus requires expression of α-toxin
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Pulmonary infection of cystic fibrosis mice with Staphylococcus aureus requires expression of α-toxin

  • Simone Keitsch , Joachim Riethmüller , Matthias Soddemann , Carolin Sehl , Barbara Wilker , Michael J. Edwards , Charles C. Caldwell , Martin Fraunholz , Erich Gulbins und Katrin Anne Becker EMAIL logo
Veröffentlicht/Copyright: 1. Mai 2018
Biological Chemistry
Aus der Zeitschrift Biological Chemistry Band 399 Heft 10

Abstract

Pulmonary infections of cystic fibrosis (CF) patients with Staphylococcus aureus (S. aureus) occur very early in the disease. The molecular details that cause infection-susceptibility of CF patients to and mediate infection with S. aureus are poorly characterized. Therefore, we aimed to identify the role of α-toxin, a major S. aureus toxin, for pulmonary infection of CF mice. Infection with S. aureus JE2 resulted in severe pneumonia in CF mice, while wildtype mice were almost unaffected. Deficiency of α-toxin in JE2-Δhla reduced the pathogenicity of S. aureus in CF mice. However, CF mice were still more susceptible to the mutant S. aureus strain than wildtype mice. The S. aureus JE2 induced a marked increase of ceramide and a downregulation of sphingosine and acid ceramidase expression in bronchi of CF mice. Deletion of α-toxin reduced these changes after infection of CF mice. Similar changes were observed in wildtype mice, but at much lower levels. Our data indicate that expression of α-toxin is a major factor causing S. aureus infections in CF mice. Wildtype S. aureus induces a marked increase of ceramide and a reduction of sphingosine and acid ceramidase expression in bronchial epithelial cells of wildtype and CF mice, changes that determine infection susceptibility.

Keywords: ceramide; cystic fibrosis; pneumonia; sphingosine; Staphylococcus aureus; toxins

Acknowledgments

We thank the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) Program supported under NIAID/NIH Contract n° HHSN272200700055C for bacterial strains. The study was supported by the GRK 2098 to K.A.B. and E.G. and DFG grants GU 335/33-1 and GU 335/35-1 to E.G. (Funder Id: 10.13039/501100001659). This article is written in the memory of our dear colleague and friend Joachim Riethmüller, who passed away in the summer of 2017.

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Received: 2018-02-18
Accepted: 2018-03-28
Published Online: 2018-05-01
Published in Print: 2018-09-25

©2018 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

  1. Frontmatter
  2. Highlight: sphingolipids in infectious biology and immunology
  3. Sphingolipids in early viral replication and innate immune activation
  4. The function of sphingomyelinases in mycobacterial infections
  5. The role of acid sphingomyelinase and modulation of sphingolipid metabolism in bacterial infection
  6. The neutral sphingomyelinase 2 in T cell receptor signaling and polarity
  7. Click reactions with functional sphingolipids
  8. Sphingolipids in inflammatory hypoxia
  9. CD4+ Foxp3+ regulatory T cell-mediated immunomodulation by anti-depressants inhibiting acid sphingomyelinase
  10. Pathological manifestations of Farber disease in a new mouse model
  11. Pulmonary infection of cystic fibrosis mice with Staphylococcus aureus requires expression of α-toxin
  12. Minireview
  13. Roles of the nucleotide exchange factor and chaperone Hsp110 in cellular proteostasis and diseases of protein misfolding
  14. Research Articles/Short Communications
  15. Proteolysis
  16. The two cathepsin B-like proteases of Arabidopsis thaliana are closely related enzymes with discrete endopeptidase and carboxydipeptidase activities
https://doi.org/10.1515/hsz-2018-0161
Schlagwörter für diesen Artikel
ceramide; cystic fibrosis; pneumonia; sphingosine; Staphylococcus aureus; toxins

Artikel in diesem Heft

  1. Frontmatter
  2. Highlight: sphingolipids in infectious biology and immunology
  3. Sphingolipids in early viral replication and innate immune activation
  4. The function of sphingomyelinases in mycobacterial infections
  5. The role of acid sphingomyelinase and modulation of sphingolipid metabolism in bacterial infection
  6. The neutral sphingomyelinase 2 in T cell receptor signaling and polarity
  7. Click reactions with functional sphingolipids
  8. Sphingolipids in inflammatory hypoxia
  9. CD4+ Foxp3+ regulatory T cell-mediated immunomodulation by anti-depressants inhibiting acid sphingomyelinase
  10. Pathological manifestations of Farber disease in a new mouse model
  11. Pulmonary infection of cystic fibrosis mice with Staphylococcus aureus requires expression of α-toxin
  12. Minireview
  13. Roles of the nucleotide exchange factor and chaperone Hsp110 in cellular proteostasis and diseases of protein misfolding
  14. Research Articles/Short Communications
  15. Proteolysis
  16. The two cathepsin B-like proteases of Arabidopsis thaliana are closely related enzymes with discrete endopeptidase and carboxydipeptidase activities
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