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Targeted degradomics in protein terminomics and protease substrate discovery

  • Simonas Savickas and Ulrich auf dem Keller EMAIL logo
Published/Copyright: August 29, 2017

Abstract

Targeted degradomics integrates positional information into mass spectrometry (MS)-based targeted proteomics workflows and thereby enables analysis of proteolytic cleavage events with unprecedented specificity and sensitivity. Rapid progress in the establishment of protease-substrate relations provides extensive degradomics target lists that now can be tested with help of selected and parallel reaction monitoring (S/PRM) in complex biological systems, where proteases act in physiological environments. In this minireview, we describe the general principles of targeted degradomics, outline the generic experimental workflow of the methodology and highlight recent and future applications in protease research.

Acknowledgments

We thank S. Werner (ETH Zurich, Switzerland) for her continuous support of our work and the Swiss National Science Foundation for funding with help of a project grant (31003A_163216).

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Received: 2017-6-28
Accepted: 2017-8-21
Published Online: 2017-8-29
Published in Print: 2017-12-20

©2018 Walter de Gruyter GmbH, Berlin/Boston

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