Heat shock protein 47 effects on hepatic stellate cell-associated receptors in hepatic fibrosis of Schistosoma japonicum-infected mice
-
Yumin Zhao
Abstract
The study aimed to explore the regulation of heat shock protein 47 (HSP47) on expressions of receptors associated with hepatic stellate cell (HSC) in liver fibrosis mouse models induced by Schistosoma japonicum (S. japonicum). Mouse fibroblasts (NIH/3T3) were transfected with HSP47 shRNA plasmid by lipofectamine transfection, and experimental fibrosis in HSCs was studied in S. japonicum mouse models treated with HSP47 shRNA in vivo. HSP47 expression was assessed using Western blot and real-time PCR. Flow cytometry was adopted to determine the expression of cell membrane receptors. HSP47-shRNA could markedly down-regulate the expression of collagen (Col1a1 and Col3a1). The expressions of HSP47, endothelin receptor A (ETAR) and endothelin receptor B (ETBR) significantly increased in the liver tissue of infected mice. However, the expressions of ETAR and HSP47 and ETBR remarkably decreased after the administration of HSP47 shRNA in vitro and in vivo. ETAR and ETBR levels were found to be positively correlated with HSP47 expression. HSP47 might exert influence on liver fibrosis via the regulation of ETAR and ETBR.
Ethical approval: This research was approved by the animal care and use committee of Guilin Medical University.
Conflict of interest statement: All the authors declare no conflict of interest.
References
Chen, J., Xu, T., Zhu, D., Wang, J., Huang, C., Lyu, L., Hu, B., Sun, W., and Duan, Y. (2016). Egg antigen p40 of Schistosoma japonicum promotes senescence in activated hepatic stellate cells by activation of the STAT3/p53/p21 pathway. Cell Death Dis. 7, e2315.10.1038/cddis.2016.228Suche in Google Scholar PubMed PubMed Central
Chu, H., Wu, T., Wu, W., Tu, W., Jiang, S., Chen, S., Ma, Y., Liu, Q., Zhou, X., Jin, L., et al. (2015). Involvement of collagen-binding heat shock protein 47 in scleroderma-associated fibrosis. Protein Cell. 6, 589–598.10.1007/s13238-015-0171-3Suche in Google Scholar PubMed PubMed Central
Duan, Y., Pan, J., Chen, J., Zhu, D., Wang, J., Sun, X., Chen, L., and Wu, L. (2016). Soluble egg antigens of Schistosoma japonicum induce senescence of activated hepatic stellate cells by activation of the FoxO3a/SKP2/P27 pathway. PLoS Negl. Trop. Dis. 10, e0005268.10.1371/journal.pntd.0005268Suche in Google Scholar PubMed PubMed Central
Duong, H.T., Dong, Z., Su, L., Boyer, C., George, J., Davis, T.P., and Wang, J. (2015). The use of nanoparticles to deliver nitric oxide to hepatic stellate cells for treating liver fibrosis and portal hypertension. Small. 11, 2291–2304.10.1002/smll.201402870Suche in Google Scholar PubMed
Duran, I., Nevarez, L., Sarukhanov, A., Wu, S., Lee, K., Krejci, P., Weis, M., Eyre, D., Krakow, D., and Cohn, D.H. (2015). HSP47 and FKBP65 cooperate in the synthesis of type I procollagen. Hum. Mol. Genet. 24, 1918–1928.10.1093/hmg/ddu608Suche in Google Scholar PubMed PubMed Central
He, C., Miao, X., Li, J., and Qi, H. (2013). Angiotensin II induces endothelin-1 expression in human hepatic stellate cells. Dig. Dis. Sci. 58, 2542–2549.10.1007/s10620-013-2685-ySuche in Google Scholar PubMed
Huang, J.Q., Tao, R., Li, L., Ma, K., Xu, L., Ai, G., Fan, X.X., Jiao, Y.T., and Ning, Q. (2014). Involvement of heat shock protein 47 in Schistosoma japonicum-induced hepatic fibrosis in mice. Int. J. Parasitol. 44, 23–35.10.1016/j.ijpara.2013.08.009Suche in Google Scholar PubMed
Ito, S. and Nagata, K. (2017). Biology of Hsp47 (Serpin H1), a collagen-specific molecular chaperone. Semin. Cell. Dev. Biol. 62, 142–151.10.1016/j.semcdb.2016.11.005Suche in Google Scholar PubMed
Kawasaki, K., Ushioda, R., Ito, S., Ikeda, K., Masago, Y., and Nagata, K. (2015). Deletion of the collagen-specific molecular chaperone Hsp47 causes endoplasmic reticulum stress-mediated apoptosis of hepatic stellate cells. J. Biol. Chem. 290, 3639–3646.10.1074/jbc.M114.592139Suche in Google Scholar PubMed PubMed Central
Lamb, C.L., Cholico, G.N., Perkins, D.E., Fewkes, M.T., Oxford, J.T., Lujan, T.J., Morrill, E.E., and Mitchell, K.A. (2016). Aryl hydrocarbon receptor activation by TCDD modulates expression of extracellular matrix remodeling genes during experimental liver fibrosis. Biomed. Res. Int. 2016, 5309328.10.1155/2016/5309328Suche in Google Scholar PubMed PubMed Central
Li, H., Hua, J., Guo, C.X., Wang, W.X., Wang, B.J., Yang, D.L., Wei, P., and Lu, Y.P. (2016). Pentoxifylline inhibits liver fibrosis via hedgehog signaling pathway. J. Huazhong Univ. Sci. Technol. Med. Sci. 36, 372–376.10.1007/s11596-016-1594-7Suche in Google Scholar PubMed
Long, X., Chen, Q., Zhao, J., Rafaels, N., Mathias, P., Liang, H., Potee, J., Campbell, M., Zhang, B., Gao, L., et al. (2015). An IL-13 promoter polymorphism associated with liver fibrosis in patients with Schistosoma japonicum. PLoS One. 10, e0135360.10.1371/journal.pone.0135360Suche in Google Scholar PubMed PubMed Central
Poosti, F., Bansal, R., Yazdani, S., Prakash, J., Post, E., Klok, P., van den Born, J., de Borst, M.H., van Goor, H., Poelstra, K., et al. (2015). Selective delivery of IFN-γ to renal interstitial myofibroblasts: a novel strategy for the treatment of renal fibrosis. FASEB J. 29, 1029–1042.10.1096/fj.14-258459Suche in Google Scholar PubMed
Sica, D.A. (2015). Mineralocorticoid receptor antagonists for treatment of hypertension and heart failure. Methodist Debakey Cardiovasc J. 11, 235–239.10.14797/mdcj-11-4-235Suche in Google Scholar PubMed PubMed Central
Tobe, S., Kohan, D.E., and Singarayer, R. (2015). Endothelin receptor antagonists: new hope for renal protection? Curr. Hypertens Rep. 17, 57.10.1007/s11906-015-0568-7Suche in Google Scholar PubMed
Wang, Y., Huang, Y., Guan, F., Xiao, Y., Deng, J., Chen, H., Chen, X., Li, J., Huang, H., and Shi, C. (2013). Hypoxia-inducible factor-1α and MAPK co-regulate activation of hepatic stellate cells upon hypoxia stimulation. PLoS One. 8, e74051.10.1371/journal.pone.0074051Suche in Google Scholar PubMed PubMed Central
Wang, M., Abais, J.M., Meng, N., Zhang, Y., Ritter, J.K., Li, P.L., and Tang, W.X. (2014a). Upregulation of cannabinoid receptor-1 and fibrotic activation of mouse hepatic stellate cells during Schistosoma J. infection: role of NADPH oxidase. Free Radic. Biol. Med. 71, 109–120.10.1016/j.freeradbiomed.2014.03.015Suche in Google Scholar PubMed PubMed Central
Wang, J., Xu, F., Zhu, D., Duan, Y., Chen, J., Sun, X., He, X., Li, P., Sun, W., and Feng, J. (2014b). Schistosoma japonicum soluble egg antigens facilitate hepatic stellate cell apoptosis by downregulating Akt expression and upregulating p53 and DR5 expression. PLoS Negl. Trop. Dis. 8, e3106.10.1371/journal.pntd.0003106Suche in Google Scholar PubMed PubMed Central
Wang, C., Xuan, X., Yao, W., Huang, G., and Jin, J. (2015). Anti-profibrotic effects of artesunate on bleomycin-induced pulmonary fibrosis in Sprague Dawley rats. Mol. Med. Rep. 12, 1291–1297.10.3892/mmr.2015.3500Suche in Google Scholar PubMed
Wu, W., Feng, A., and Huang, Y. (2015). Research and control of advanced schistosomiasis japonica in China. Parasitol. Res. 114, 17–27.10.1007/s00436-014-4225-xSuche in Google Scholar PubMed
Xiang, X.H., Jiang, T.P., Zhang, S., Song, J., Li, X., Yang, J.Y., and Zhou, S. (2015). Pirfenidone inhibits proliferation, arrests the cell cycle, and downregulates heat shock protein-47 and collagen type I in rat hepatic stellate cells in vitro. Mol. Med. Rep. 12, 309–314.10.3892/mmr.2015.3403Suche in Google Scholar PubMed
Xiao, X., Cao, L., Wang, R., Shen, Z.X., and Cao, Y.X. (2016). Airborne fine particulate matter alters the expression of endothelin receptors in rat coronary arteries. Environ. Pollut. 218, 487–496.10.1016/j.envpol.2016.07.028Suche in Google Scholar PubMed
Yang, Y., Kim, B., Park, Y.K., Koo, S.I., and Lee, J.Y. (2015). Astaxanthin prevents TGFβ1-induced pro-fibrogenic gene expression by inhibiting Smad3 activation in hepatic stellate cells. Biochim. Biophys. Acta. 1850, 178–185.10.1016/j.bbagen.2014.10.014Suche in Google Scholar PubMed
Yokomori, H., Oda, M., Yasogawa, Y., Nishi, Y., Ogi, M., Takahashi, M., and Ishii, H. (2001). Enhanced expression of endothelin B receptor at protein and gene levels in human cirrhotic liver. Am. J. Pathol. 159, 1353–1362.10.1016/S0002-9440(10)62522-9Suche in Google Scholar PubMed PubMed Central
©2017 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Reviews
- Regulation of protein function by S-nitrosation and S-glutathionylation: processes and targets in cardiovascular pathophysiology
- Cystine knot growth factors and their functionally versatile proregions
- Kallistatin: double-edged role in angiogenesis, apoptosis and oxidative stress
- Minireview
- The sphingomyelin synthase family: proteins, diseases, and inhibitors
- Research Articles/Short Communications
- Genes and Nucleic Acids
- Comparison of cytochrome P450 expression in four different human osteoblast models
- Cell Biology and Signaling
- The molecular mechanisms involved in lectin-induced human platelet aggregation
- HDAC1 triggers the proliferation and migration of breast cancer cells via upregulation of interleukin-8
- Heat shock protein 47 effects on hepatic stellate cell-associated receptors in hepatic fibrosis of Schistosoma japonicum-infected mice
Artikel in diesem Heft
- Frontmatter
- Reviews
- Regulation of protein function by S-nitrosation and S-glutathionylation: processes and targets in cardiovascular pathophysiology
- Cystine knot growth factors and their functionally versatile proregions
- Kallistatin: double-edged role in angiogenesis, apoptosis and oxidative stress
- Minireview
- The sphingomyelin synthase family: proteins, diseases, and inhibitors
- Research Articles/Short Communications
- Genes and Nucleic Acids
- Comparison of cytochrome P450 expression in four different human osteoblast models
- Cell Biology and Signaling
- The molecular mechanisms involved in lectin-induced human platelet aggregation
- HDAC1 triggers the proliferation and migration of breast cancer cells via upregulation of interleukin-8
- Heat shock protein 47 effects on hepatic stellate cell-associated receptors in hepatic fibrosis of Schistosoma japonicum-infected mice
