Abstract
Somatic cells can be reprogramed into induced pluripotent stem (iPS) cells by defined factors, which provide a powerful basis for personalized stem-cell based therapies. However, cellular reprograming is an inefficient and metabolically demanding process commonly associated with obstacles that hamper further use of this technology. Spontaneous differentiation of iPS cells cultures represents a significant hurdle that hinder obtaining high quality iPS cells for further downstream experimentation. In this study, we found that a natural compound, vitamin C, augmented pluripotency in iPS cells and reduced unwanted spontaneous differentiation during iPS cells maintenance. Gene expression analysis showed that vitamin C increased the expression of the histone demethylase JARID1A. Furthermore, through gain- and loss-of-function approaches, we show that JARID1A is a key effector in promoting pluripotency and reducing differentiation downstream of vitamin C. Our results therefore highlight a straightforward method for improving the pluripotency and quality of iPS cells; it also shows a possible role for H3K4me2/3 in cell fate determination and establishes a link between vitamin C and epigenetic regulation.
Acknowledgments
The authors wish to thank Dr. Stefano Ferrari for his technical assistance.
References
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Supplemental Material:
The online version of this article (DOI: 10.1515/hsz-2016-0181) offers supplementary material, available to authorized users.
©2016 Walter de Gruyter GmbH, Berlin/Boston
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- Vitamin C promotes pluripotency of human induced pluripotent stem cells via the histone demethylase JARID1A
Artikel in diesem Heft
- Frontmatter
- Reviews
- Dynamic organization of the mitochondrial protein import machinery
- Common therapeutic strategies for prion and Alzheimer’s diseases
- IL-1 family cytokines in cancer immunity – a matter of life and death
- Research Articles/Short Communications
- Genes and Nucleic Acids
- Epigenetic regulation of KLK7 gene expression in pancreatic and cervical cancer cells
- Molecular Medicine
- Regulation of glycosylphosphatidylinositol-anchored proteins and GPI-phospholipase D in a c-Myc transgenic mouse model of hepatocellular carcinoma and human HCC
- Biological characteristics of renal cancer cells after CTP-mediated cancer suppressor gene NPRL2 protein treatment
- Cell Biology and Signaling
- Hepatitis B virus surface protein-induced hPIAS1 transcription requires TAL1, E47, MYOG, NFI, and MAPK signal pathways
- Inhibition of interleukin-3- and interferon- α-induced JAK/STAT signaling by the synthetic α-X-2′,3,4,4′-tetramethoxychalcones α-Br-TMC and α-CF3-TMC
- Vitamin C promotes pluripotency of human induced pluripotent stem cells via the histone demethylase JARID1A