Abstract
Septins form oligomeric complexes consisting of septins from different subgroups, which form filaments that are involved in a number of biological processes. They are GTP-binding proteins that contain all the necessary elements to perform the general GDP-to-GTP conformational switch. It is however unclear whether or not such a switch is important for the dynamics of septin filaments. Here we investigate the complex GTPase reaction of members of each of the four human septin groups, which is dominated by the stability of dimer formation via the nucleotide binding or so-called G-interface. The results also show that the actual hydrolysis reaction is very similar for three septin groups in the monomeric state while the Sept6 has no GTPase activity. Sept7, the only member of the Sept7 subgroup, forms a very tight G-interface dimer in the GDP-bound state. Here we show that the stability of the interface is dramatically decreased by exchanging GDP with a nucleoside triphosphate, which is believed to influence filament formation and dynamics via Sept7.
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©2014 by Walter de Gruyter Berlin Boston
Artikel in diesem Heft
- Masthead
- Masthead
- Guest Editorial
- Highlight: The 5th International Workshop on Septin Biology
- Highlight: The 5th International Workshop on Septin Biology
- Septin functions in organ system physiology and pathology
- Septins in the glial cells of the nervous system
- Distinct localization of septin proteins to ciliary sub-compartments in airway epithelial cells
- Septin 9 amplification and isoform-specific expression in peritumoral and tumor breast tissue
- Human septin isoforms and the GDP-GTP cycle
- Reviews
- Are all granzymes cytotoxic in vivo?
- The role of oxidative stress during inflammatory processes
- Minireview
- Enrichment of target sequences for next-generation sequencing applications in research and diagnostics
- Research Articles/Short Communications
- Protein Structure and Function
- Roasting and lipid binding provide allergenic and proteolytic stability to the peanut allergen Ara h 8
Artikel in diesem Heft
- Masthead
- Masthead
- Guest Editorial
- Highlight: The 5th International Workshop on Septin Biology
- Highlight: The 5th International Workshop on Septin Biology
- Septin functions in organ system physiology and pathology
- Septins in the glial cells of the nervous system
- Distinct localization of septin proteins to ciliary sub-compartments in airway epithelial cells
- Septin 9 amplification and isoform-specific expression in peritumoral and tumor breast tissue
- Human septin isoforms and the GDP-GTP cycle
- Reviews
- Are all granzymes cytotoxic in vivo?
- The role of oxidative stress during inflammatory processes
- Minireview
- Enrichment of target sequences for next-generation sequencing applications in research and diagnostics
- Research Articles/Short Communications
- Protein Structure and Function
- Roasting and lipid binding provide allergenic and proteolytic stability to the peanut allergen Ara h 8