Abstract
Targeted re-sequencing such as gene panel sequencing (GPS) has become very popular in medical genetics, both for research projects and in diagnostic settings. The technical principles of the different enrichment methods have been reviewed several times before; however, new enrichment products are constantly entering the market, and researchers are often puzzled about the requirement to take decisions about long-term commitments, both for the enrichment product and the sequencing technology. This review summarizes important considerations for the experimental design and provides helpful recommendations in choosing the best sequencing strategy for various research projects and diagnostic applications.
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- Minireview
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Articles in the same Issue
- Masthead
- Masthead
- Guest Editorial
- Highlight: The 5th International Workshop on Septin Biology
- Highlight: The 5th International Workshop on Septin Biology
- Septin functions in organ system physiology and pathology
- Septins in the glial cells of the nervous system
- Distinct localization of septin proteins to ciliary sub-compartments in airway epithelial cells
- Septin 9 amplification and isoform-specific expression in peritumoral and tumor breast tissue
- Human septin isoforms and the GDP-GTP cycle
- Reviews
- Are all granzymes cytotoxic in vivo?
- The role of oxidative stress during inflammatory processes
- Minireview
- Enrichment of target sequences for next-generation sequencing applications in research and diagnostics
- Research Articles/Short Communications
- Protein Structure and Function
- Roasting and lipid binding provide allergenic and proteolytic stability to the peanut allergen Ara h 8