Startseite Lebenswissenschaften Effects of selective hypothermia on blood-brain barrier integrity and tight junction protein expression levels after intracerebral hemorrhage in rats
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Effects of selective hypothermia on blood-brain barrier integrity and tight junction protein expression levels after intracerebral hemorrhage in rats

  • Hongwei Sun , Ying Tang , Xiqin Guan , Lanfeng Li und Desheng Wang EMAIL logo
Veröffentlicht/Copyright: 3. Juli 2013

Abstract

Hypothermia has neuroprotective effects on global cerebral ischemic injuries. However, its efficacy after intracerebral hemorrhage (ICH) is inconclusive. In this study, bacterial collagenase was used to induce ICH stroke in male Wistar rats. We assessed the effects of normothermia and 4 h of local hypothermia (∼33.2°C) initiated 1, 6, or 12 h after collagenase infusion on hemorrhage volume and neurological outcomes. Following early cooling initiated after 1 h, blood-brain barrier (BBB) disruption and brain water content were tested. Furthermore, the expression levels of tight junction (TJ) proteins (claudin 5 and occludin) and the proinflammatory cytokines interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) were determined using Western blotting, real-time quantitative PCR, and immunohistochemical staining at 1 and 3 d after ICH. Early local hypothermia tends to reduce hemorrhagic volume and neurological deficits, but the difference is not statistically significant compared with other groups. However, early hypothermia significantly reduces BBB disruption, edema formation, the expression levels of IL-1β and TNF-α, and the loss of TJ proteins. Together, these data suggest that local hypothermia is an effective treatment for edema formation and BBB disruption via the upregulation of TJ proteins and the suppression of TNF-α and IL-1β.


Corresponding author: Desheng Wang, Department of Neurology, First Affiliated Hospital of Harbin Medical University, Youzheng Street, Nangang District, Harbin 150001, P.R. China

This work was supported by the Natural Science Foundation of China, No. 30570620. The authors greatly appreciate the technical assistance of Dr. Sheng Li.

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Received: 2013-2-23
Accepted: 2013-7-2
Published Online: 2013-07-03
Published in Print: 2013-10-01

©2013 by Walter de Gruyter Berlin Boston

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