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Possible role of a septin, SEPT1, in spreading in squamous cell carcinoma DJM-1 cells

  • Yoko Mizutani , Hidenori Ito , Ikuko Iwamoto , Rika Morishita , Hiroyuki Kanoh , Mariko Seishima and Koh-ichi Nagata EMAIL logo
Published/Copyright: January 8, 2013
Biological Chemistry
From the journal Biological Chemistry Volume 394 Issue 2

Abstract

We performed biochemical, histochemical and cell biological characterization of septins by focusing on SEPT1 in human skin tissues and a squamous cell carcinoma (SCC) cell line DJM-1. In immunoblotting, SEPT1, together with other septins, was detected in normal human epidermis, SCC and DJM-1. In immunohistochemical analyses, SEPT1 was detected diffusely in the cytoplasm of human epidermal cells and eccrine gland epithelial cells, and the protein level was increased in some skin tumors. In DJM-1 cells, SEPT1 together with other members of SEPT2-subgroup, SEPT4 and SEPT5, was enriched in lamellipodia and the localization was dependent on the cortical actin structure. SEPT1 distribution at lamellipodia was also observed in melanoma B16 cells. SEPT9, SEPT11 and SEPT14, in contrast, were localized along with microtubules in DJM-1 cells. In immunoprecipitation assays, SEPT1 and SEPT5 were found to form a complex in DJM-1 cells, whereas SEPT9, SEPT11 and SEPT14 formed a distinct complex with other septins including SEPT7, SEPT8 and SEPT10, in which SEPT5 was not included. When SEPT1 was silenced in DJM-1 cells, cell spreading was inhibited. These results suggest that SEPT1 may participate in cell-cell and/or cell-substrate interaction in DJM-1 and exert its function in a coordinated manner with other septins.

Keywords: cancer; human skin; polarity; septin
Corresponding author: Koh-ichi Nagata, Institute for Developmental Research, Aichi Human Service Center, Kasugai, Aichi 480-0392, Japan
Received: 2012-7-24
Accepted: 2012-10-11
Published Online: 2013-01-08
Published in Print: 2013-02-01

©2013 by Walter de Gruyter Berlin Boston

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https://doi.org/10.1515/hsz-2012-0258
Keywords for this article
cancer; human skin; polarity; septin

Articles in the same Issue

  1. Masthead
  2. Masthead
  3. Guest Editorial
  4. Highlight: The physiology and dynamics of cellular microcompartments
  5. Highlight: The Physiology and Dynamics of Cellular Microcompartments
  6. Cellular microcompartments constitute general suborganellar functional units in cells
  7. Dynamics of bioenergetic microcompartments
  8. Microcompartments within the yeast plasma membrane
  9. Plant cell microcompartments: a redox-signaling perspective
  10. Microcompartments in the Drosophila heart and the mammalian brain: general features and common principles
  11. Minireviews
  12. Beyond anemia: hepcidin, monocytes and inflammation
  13. Melanoma resistance to photodynamic therapy: new insights
  14. The biosynthesis of caprazamycins and related liponucleoside antibiotics: new insights
  15. cCMP, cUMP, cTMP, cIMP and cXMP as possible second messengers: Development of a hypothesis based on studies with soluble guanylyl cyclase α1β1
  16. Research Articles/Short Communications
  17. Protein Structure and Function
  18. Degradation of channelopsin-2 in the absence of retinal and degradation resistance in certain mutants
  19. Cell Biology and Signaling
  20. Possible role of a septin, SEPT1, in spreading in squamous cell carcinoma DJM-1 cells
  21. Proteolysis
  22. ADAMTS: Novel proteases expressed by activated mast cells
  23. The proinflammatory cytokines interleukin-1α and tumor necrosis factor α promote the expression and secretion of proteolytically active cathepsin S from human chondrocytes
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