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PhoB transcriptional activator binds hierarchically to pho box promoters
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Alexandre G. Blanco
Veröffentlicht/Copyright:
8. September 2012
Abstract
The PhoR-PhoB phosphorelay is a bacterial two-component system that activates the transcription of several genes involved in phosphate uptake and assimilation. The response begins with the autophosphorylation of the sensor kinase PhoR, which activates the response regulator PhoB. Upon binding to the pho box DNA sequence, PhoB recruits the RNA polymerase and thereby activates the transcription of specific genes. To unveil hitherto unknown molecular mechanisms along the activation pathway, we report biochemical data characterizing the PhoB binding to promoters containing multiple pho boxes and describe the crystal structure of two PhoB DNA-binding domains bound in tandem to a 26-mer DNA.
Keywords: PhoB oligomerization state; RNA polymerase sigma 70; transcription activation; two-component signal transduction; X-ray crystal structure
Received: 2012-6-13
Accepted: 2012-8-20
Published Online: 2012-09-08
Published in Print: 2012-10-01
©2012 by Walter de Gruyter Berlin Boston
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Schlagwörter für diesen Artikel
PhoB oligomerization state;
RNA polymerase sigma 70;
transcription activation;
two-component signal transduction;
X-ray crystal structure
Artikel in diesem Heft
- Masthead
- Masthead
- Guest Editorial
- No end in sight: the development of protein crystallography in Martinsried
- Highlight: No End in Sight: The Development of Protein Crystallography in Martinsried
- Functional and structural insights into astacin metallopeptidases
- β-Trefoil inhibitors – from the work of Kunitz onward
- Structural basis of the TAL effector–DNA interaction
- Nature’s way of handling a greenhouse gas: the copper-sulfur cluster of purple nitrous oxide reductase
- Rustless translation
- Structural basis of [NiFe] hydrogenase maturation by Hyp proteins
- Covalent and non-covalent reversible proteasome inhibition
- Structural origins of AGC protein kinase inhibitor selectivities: PKA as a drug discovery tool
- Structural analysis of the C-terminal domain of the spliceosomal helicase Prp22
- N-terminal acetylation of annexin A2 is required for S100A10 binding
- Withaferin A binds covalently to the N-terminal domain of annexin A2
- PhoB transcriptional activator binds hierarchically to pho box promoters
- Research Articles/Short Communications
- Protein Structure and Function
- Biochemical and functional characterization of human phospholipid scramblase 4 (hPLSCR4)
- Cell Biology and Signaling
- Dual-specificity phosphatases are targets of the Wnt/β-catenin pathway and candidate mediators of β-catenin/Ras signaling interactions
- Proteolysis
- Inhibition of Aeromonas sobria serine protease (ASP) by α2-macroglobulin
