Threshold values of antibodies to estrogen, progesteron and benzo [a] pyrene as a risk factor for the development of endometriosis

Natalia V. Artymuk; Vitaliy O. Chervov; Larissa N. Danilova; Elena G. Polenok; Olga Zotova

doi:10.1515/hmbci-2020-0056

Article

Threshold values of antibodies to estrogen, progesteron and benzo [a] pyrene as a risk factor for the development of endometriosis

Natalia V. Artymuk , Vitaliy O. Chervov , Larissa N. Danilova , Elena G. Polenok and Olga Zotova

Published/Copyright: March 9, 2021

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From the journal Hormone Molecular Biology and Clinical Investigation Volume 42 Issue 3

Abstract

Objectives

The objective of the study was to determine the level of antibodies (AB) of Ig classes A and G to estradiol (E2), progesterone (P) and benzo [a] pyrene (Bp) in patients with endometriosis of various severity and estimate their threshold values as a risk factor for the development of endometriosis.

Methods

A retrospective case–control study was performed. The study involved 200 women. Group I: women with endometriosis (n=100), Group II: patients with tubal-peritoneal infertility (n=100). All patients underwent immunological studies of blood serum; and the levels of steroid hormones (P, E2), antibodies to them and Bp were determined. A ROC analysis was carried out to identify threshold values of antibodies levels.

Results

Women with endometriosis were found to have statistically significantly higher levels of antibodies IgA and IgG to E2, P and benzo [a] pyrene compared to women of Group II. The threshold levels of IgA-Bp, IgA-E2 and IgA-P are >5 CU (conventional unit), IgG-Bp, IgG-E2>9 CU and IgG-P>8 CU. The level of IgG-P in patients with severe forms of endometriosis is statistically significantly higher than in minor forms of the disease. In case of severe forms, there is a tendency to increasing other classes of antibodies.

Conclusions

Patients with endometriosis usually have a higher level of IgA and IgG to Bp, E2, P. Their threshold values, which are risk factors for the development of the disease, are estimated.

Keywords: antibodies; benzo [a] pyrene; endometriosis; estrogen; progesterone; xenobiotics

Corresponding author: Olga Zotova, PhD, Gynecologist, L. Reshetova Kemerovo Regional Perinatal Center, Oktyabrsky Prospect, 22B, Kemerovo, 650065, Russia, Phone: +8 923 486 92 24, Fax: +7(3842)392279, E-mail: Olga-tulpan@rambler.ru

Research funding: The study was conducted without sponsorship.
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: The authors declare no potential conflicts of financial and other interests.
Informed consent: Informed consent was obtained from all participants.
Ethical approval: The study was approved by the Committee on Ethics and Evidence of Medical Research of the Kemerovo State Medical University (KemSMU) and met the ethical standards of the Bioethical Committee that were developed in accordance with the Declaration of Helsinki (DoH) of the World Medical Association (WMA): “Declaration of Helsinki: Ethical Principles for Medical Research Involving Human Subjects” amended in 2013 and “The Rules for Clinical Practice in the Russian Federation”, approved by the Order of the Ministry of Health of Russia No. 266, June 19, 2003.

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Received: 2020-08-02

Accepted: 2021-02-18

Published Online: 2021-03-09

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Articles in the same Issue

https://doi.org/10.1515/hmbci-2020-0056

Keywords for this article

antibodies; benzo [a] pyrene; endometriosis; estrogen; progesterone; xenobiotics