Abstract
Cell free DNA (cf-DNA) refers to all non -ncapsulated DNA present in the blood stream which may originate from apoptotic cells as a part of the physiological cell turnover, or from cancer cells or fetal cells. Recent studies have highlighted the utility of cfDNA analysis for genetic profiling of cancer, non-invasive prenatal testing besides many other clinical applications. In our review we discuss the sources of cfDNA in the body, the techniques most commonly being used for its isolation and analysis, the applications of cfDNA testing and the associated pros-cons. We conclude that for prenatal testing, cfDNA analysis provides an effective, non-invasive and safer alternative to traditional amniocentesis and chorionic villus sampling tests. Also, in cancer patients, cfDNA analysis is useful for genetic profiling and follow-up during treatment. However, standardization of methods of isolation and analysis has become crucial for the success of widespread use of cfDNA analysis.
Author statement
Research funding: None declared.
Conflict of interest: None declared.
Informed consent: Not applicable.
Ethical approval: Not applicable.
References
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Artikel in diesem Heft
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- Studying non-alcoholic fatty liver disease: the ins and outs of in vivo, ex vivo and in vitro human models
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- Implementation of a novel self-induced promoter for the expression of pharmaceutical peptides in Escherichia coli: YY(3-36) peptide
- Comparison of the effect of 12- and 24-session cardiac rehabilitation on physical, psychosocial and biomedical factors in ischemic heart disease patients
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- Review Articles
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Artikel in diesem Heft
- Special Issue: ‘Liver Metabolic Diseases and Hepatocellular Carcinoma: New Hormonal and Clinical Insights’ / Editors: Gérard S. Chetrite and Bruno Féve
- Editorial Preface
- Preface to special issue on “Liver Metabolic Diseases and Hepatocellular Carcinoma: New Hormonal and Clinical Insights”
- Original Article
- Aromatase in normal and diseased liver
- Review Articles
- Hepatocellular carcinoma in the context of non-alcoholic steatohepatitis (NASH): recent advances in the pathogenic mechanisms
- Studying non-alcoholic fatty liver disease: the ins and outs of in vivo, ex vivo and in vitro human models
- Metalloproteinases in non-alcoholic fatty liver disease and their behavior in liver fibrosis
- Hypothyroidism and nonalcoholic fatty liver disease – a chance association?
- Mini-Review Article
- The impact of steatosis on liver regeneration
- Regular Issue
- Original Articles
- Hepatoprotective effects of Shilajit on high fat-diet induced non-alcoholic fatty liver disease (NAFLD) in rats
- 3-Iodothyronamine and 3,5,3′-triiodo-L-thyronine reduce SIRT1 protein expression in the HepG2 cell line
- Machine learning as new promising technique for selection of significant features in obese women with type 2 diabetes
- Implementation of a novel self-induced promoter for the expression of pharmaceutical peptides in Escherichia coli: YY(3-36) peptide
- Comparison of the effect of 12- and 24-session cardiac rehabilitation on physical, psychosocial and biomedical factors in ischemic heart disease patients
- The stimulation protocol in poor responder IVF; a minimal or high-dose stimulation? – A meta-analysis
- Review Articles
- Cell free DNA: revolution in molecular diagnostics – the journey so far
- Genitourinary syndrome of menopause (GSM) and laser VEL: a review