Dissolution and acetylation of ball-milled birch (Betula platyphylla) and bamboo (Phyllostachys nigra) in the ionic liquid [Bmim]Cl for HSQC NMR analysis
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Chen Qu
,
Shinjiro Ogita
Abstract
A method for nuclear magnetic resonance (NMR) characterization of whole cell wall components, including lignin, cellulose and hemicelluloses, was recently developed in our laboratory. The method described for fir (Abies sachalinensis) as a softwood consists of ball-milling of cell wall, dissolution in an ionic liquid 1-butyl-3-methylimidazolium chloride ([Bmim]Cl), in situ acetylation, recovery of the material from the solution, and characterization of the product by 1H-13C correlation heteronuclear single quantum coherence (HSQC) NMR spectroscopy in dimethyl sulfoxide (DMSO)-d6. In the present paper, the performance of the method should be tested for a hardwood and a bamboo. Thus, Japanese white birch (Betula platyphylla) and hachiku bamboo (Phyllostachys nigra) have been investigated. Finely ball-milled birch and bamboo materials were completely dissolved in [Bmim]Cl at 100°C without severe chemical modification of the cell wall components. The dissolved cell walls were then subjected to in situ acetylation, and the ball-milled and fully acetylated cell walls were recovered from [Bmim]Cl. Longer ball-milling time was required for birch and bamboo cell walls, because of the lower solubility of acetylated birch and bamboo materials in DMSO-d6compared to the acetylated fir material. However, HSQC NMR experiments were successfully conducted, and the acetylated whole cell wall components in the birch and bamboo could be fully characterized. This method is applicable for the analysis of cell wall components of various plant biomasses without previous isolation. Further studies are necessary to improve the method.
©2012 by Walter de Gruyter Berlin Boston
Artikel in diesem Heft
- Original Papers
- Identification of new lignans in Norway spruce knotwood extracts
- Isolation and characterization of β-cadinene synthase cDNA from Chamaecyparis formosensis Matsum
- Antioxidant activity of fractions from Quercus sideroxyla bark and identification of proanthocyanidins by HPLC-DAD and HPLC-MS
- New diarylpropanoids from Lindera glauca Bl. heartwood
- Extracting value-added products before pulping: Hemicellulosic ethanol from Eucalyptus globulus wood
- Effect of various pulp properties on the solubility of cellulose in sodium hydroxide solutions
- Dissolution and acetylation of ball-milled birch (Betula platyphylla) and bamboo (Phyllostachys nigra) in the ionic liquid [Bmim]Cl for HSQC NMR analysis
- Study of thin films of kraft lignin and two DHPs by means of single-molecule force spectroscopy (SMFS)
- Characterization of mixed-mode I/II fracture properties of adhesively bonded yellow-poplar by a dual actuator test frame instrument
- Grading of structural timber of Populus × euramericana clone I-214
- Measurement of wood shrinkage in jack pine using three dimensional digital image correlation (DIC)
- Internal stress measurement during drying of rubberwood lumber: effects of wet-bulb temperature in various drying strategies
- Tensile behaviour of radiata pine with different moisture contents at elevated temperatures
- Within-process and seasonal changes during industrial production of high-density fibreboard. Part 1: Influence of wood species composition on polyoses in the products
- Within-process and seasonal changes during industrial production of high-density fibreboard. Part 2: PLS modelling of chemical alterations, refining conditions and panel thickness swell
- Short Note
- Effect of grain angle on shear strength of Douglas-fir wood
- Meetings
- Meetings
- Masthead
- Masthead
Artikel in diesem Heft
- Original Papers
- Identification of new lignans in Norway spruce knotwood extracts
- Isolation and characterization of β-cadinene synthase cDNA from Chamaecyparis formosensis Matsum
- Antioxidant activity of fractions from Quercus sideroxyla bark and identification of proanthocyanidins by HPLC-DAD and HPLC-MS
- New diarylpropanoids from Lindera glauca Bl. heartwood
- Extracting value-added products before pulping: Hemicellulosic ethanol from Eucalyptus globulus wood
- Effect of various pulp properties on the solubility of cellulose in sodium hydroxide solutions
- Dissolution and acetylation of ball-milled birch (Betula platyphylla) and bamboo (Phyllostachys nigra) in the ionic liquid [Bmim]Cl for HSQC NMR analysis
- Study of thin films of kraft lignin and two DHPs by means of single-molecule force spectroscopy (SMFS)
- Characterization of mixed-mode I/II fracture properties of adhesively bonded yellow-poplar by a dual actuator test frame instrument
- Grading of structural timber of Populus × euramericana clone I-214
- Measurement of wood shrinkage in jack pine using three dimensional digital image correlation (DIC)
- Internal stress measurement during drying of rubberwood lumber: effects of wet-bulb temperature in various drying strategies
- Tensile behaviour of radiata pine with different moisture contents at elevated temperatures
- Within-process and seasonal changes during industrial production of high-density fibreboard. Part 1: Influence of wood species composition on polyoses in the products
- Within-process and seasonal changes during industrial production of high-density fibreboard. Part 2: PLS modelling of chemical alterations, refining conditions and panel thickness swell
- Short Note
- Effect of grain angle on shear strength of Douglas-fir wood
- Meetings
- Meetings
- Masthead
- Masthead
