Abstract
The process named “sulfite pretreatment to overcome recalcitrance of lignocellulose” (SPORL) is designed for the digestion of softwoods within the framework of the biorefinery concept. “Hot water extraction (HWE)” is an environmentally benign and low-cost pretreatment, which only needs water as a reagent. In the present study, HWE has been investigated as a pre-process prior to SPORL with Douglas fir as feedstock. The SPORL parameters, namely, temperature and treatment time, were in the range 135–155°C and 40–120 min, respectively, while the sulfuric acid concentration was 0.2–0.4% (v/v). The aim was to maximize the enzymatic digestibility of the treated wood. The severity of SPORL at different pretreatment conditions was characterized by the combined severity factor (CSF). The HWE pre-process led to a two-fold increase in specific surface area of the substrate. More hemicellulosic-derived simple sugars were dissolved in the spent liquor (SL) as the CSF increased from 1.23 to 1.82. A maximum enzymatic digestibility of 64.3% was attained when SPORL was conducted at 155°C for 120 min with a sulfuric acid concentration of 0.4% (v/v). A considerably high enzymatic digestibility (~55–60%) is still achievable by incorporating HWE prior to SPORL, even if the SPORL severity is reduced, namely to a lower temperature (145°C), a shorter time (80 min), and a lower acid volume (0.3% v/v).
Acknowledgments
This work, as part of the Northwest Advanced Renewables Alliance (NARA), was supported by the Agriculture and Food Research Initiative Competitive Grant no. 2011-68005-30416 from the United States Department of Agriculture (USDA), National Institute of Food and Agriculture.
References
Alizadeh, H., Teymouri, F., Gilbert, T.I., Dale, B.E. (2005) Pretreatment of switchgrass by ammonia fiber explosion (AFEX). Appl. Biochem. Biotechnol. 124:1133–1141.10.1007/978-1-59259-991-2_94Suche in Google Scholar
Alvira, P., Tomás-Pejó, E., Ballesteros, M., Negro, M.J. (2010) Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: a review. Bioresour. Technol. 101:4851–4861.10.1016/j.biortech.2009.11.093Suche in Google Scholar PubMed
Amidon, T.E., Wood, C.D., Shupe, A.M. (2008) Biorefinery: conversion of woody biomass to chemicals, energy and materials. J. Biobased Mater. Bioenergy. 2:100–120.10.1166/jbmb.2008.302Suche in Google Scholar
Assor, C., Placet, V., Chabbert, B., Habrant, A. (2009) Concomitant changes in viscoelastic properties and amorphous polymers during the hydrothermal treatment of hardwood and softwood. J. Agric. Food Chem. 57:6830–6837.10.1021/jf901373sSuche in Google Scholar PubMed
Bianchi, S., Koch, G., Janzon, R., Mayer, I., Saake, B., Pichelin, F. (2016) Hot water extraction of Norway spruce (Picea abies [Karst.]) bark: analyses of the influence of bark aging and process parameters on the extract composition. Holzforschung 70:619–631.10.1515/hf-2015-0160Suche in Google Scholar
Blumentritt, M., Gardner, D.J., Cole, B.J.W., Shaler, S.M. (2016) Influence of hot-water extraction on ultrastructure and distribution of glucomannans and xylans in poplar xylem as detected by gold immunolabeling. Holzforschung 70:243–252.10.1515/hf-2015-0030Suche in Google Scholar
Brunauer, S., Emmett, P.H., Teller, E. (1938) Adsorption of Gases in Multimolecular Layers. J. Am. Chem. Soc. 60:309–319.10.1021/ja01269a023Suche in Google Scholar
Cantarella, M., Cantarella, L., Gallifuoco, A., Spera, A., Alfani, F. (2004) Effect of Inhibitors Released during Steam-Explosion Treatment of Poplar Wood on Subsequent Enzymatic Hydrolysis and SSF. Biotechnol. Prog. 20:200–206.10.1021/bp0257978Suche in Google Scholar PubMed
Cateto, C., Hu, G., Ragauskas, A. (2011) Enzymatic hydrolysis of organosolv Kanlow switchgrass and its impact on cellulose crystallinity and degree of polymerization. Energy Environ. Sci. 4:1516–1521.10.1039/c0ee00827cSuche in Google Scholar
Chaffee, T.L. (2011) Potential for enhanced properties of wood products by hot water extraction of low-value, undebarked ponderosa pine. SUNY College of Environmental Science Forestry, Syracuse. 81:1496412.Suche in Google Scholar
Dautzenberg, G., Gerhardt, M., Kamm, B. (2011) Bio based fuels and fuel additives from lignocellulose feedstock via the production of levulinic acid and furfural. Holzforschung 65:439–451.10.1515/hf.2011.081Suche in Google Scholar
Fengel, D., Wegener, G. (1983) Wood: chemistry, ultrastructure, reactions. de Gruyter.10.1515/9783110839654Suche in Google Scholar
Goh, C.S., Lee, K.T., Bhatia, S. (2010) Hot compressed water pretreatment of oil palm fronds to enhance glucose recovery for production of second generation bio-ethanol. Bioresour. Technol. 101:7362–7367.10.1016/j.biortech.2010.04.048Suche in Google Scholar PubMed
Himmel, M.E., Ding, S.Y., Johnson, D.K., Adney, W.S., Nimlos, M.R., Brady, J.W., Foust, T.D. (2007) Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production. Science 315:804–807.10.1126/science.1137016Suche in Google Scholar PubMed
Hörhammer, H., Walton, S., van Heiningen, A. (2011) A larch based biorefinery: pre-extraction and extract fermentation to lactic acid. Holzforschung 65:491–496.10.1515/hf.2011.085Suche in Google Scholar
Horn, S.J., Eijsink, V.G.H. (2010) Enzymatic Hydrolysis of Steam-Exploded Hardwood Using Short Processing Times. Biosci. Biotechnol. Biochem. 74:1157–1163.10.1271/bbb.90762Suche in Google Scholar PubMed
Jeoh, T., Ishizawa, C.I., Davis, M.F., Himmel, M.E., Adney, W.S., Johnson, D.K. (2007) Cellulase digestibility of pretreated biomass is limited by cellulose accessibility. Biotechnol. Bioeng. 98:112–122.10.1002/bit.21408Suche in Google Scholar PubMed
Kleen, M., Pranovich, A., Willför, S. (2016) Statistical modeling of pressurized hot-water batch extraction (PHWE) to produce hemicelluloses with desired properties. Holzforschung 70:633–640.10.1515/hf-2015-0048Suche in Google Scholar
Lan, T.Q., Gleisner, R., Zhu, J.Y., Dien, B.S., Hector, R.E. (2013a) High titer ethanol production from SPORL-pretreated lodgepole pine by simultaneous enzymatic saccharification and combined fermentation. Bioresour. Technol. 127:291–297.10.1016/j.biortech.2012.09.111Suche in Google Scholar PubMed
Lan, T.Q., Lou, H., Zhu, J.Y. (2013b) Enzymatic Saccharification of Lignocelluloses Should be Conducted at Elevated pH 5.2–6.2. Bioenergy Res. 6:476–485.10.1007/s12155-012-9273-4Suche in Google Scholar
Larsson, S., Reimann, A., Nilvebrant, N.-O., Jönsson, L.J. (1999) Comparison of different methods for the detoxification of lignocellulose hydrolyzates of spruce. Appl. Biochem. Biotechnol. 77:91–103.10.1007/978-1-4612-1604-9_9Suche in Google Scholar
Lee, S.-H., Chang, F., Inoue, S., Endo, T. (2010) Increase in enzyme accessibility by generation of nanospace in cell wall supramolecular structure. Bioresour. Technol. 101:7218–7223.10.1016/j.biortech.2010.04.069Suche in Google Scholar
Lehto, J., Alén, R. (2015) Organic materials in black liquors of soda-AQ pulping of hot-water-extracted birch (Betula pendula) sawdust. Holzforschung 69:257–264.10.1515/hf-2014-0094Suche in Google Scholar
Leu, S.-Y., Zhu, J.Y., Gleisner, R., Sessions, J., Marrs, G. (2013) Robust enzymatic saccharification of a Douglas-fir forest harvest residue by SPORL. Biomass and Bioenergy 59:393–401.10.1016/j.biombioe.2013.08.014Suche in Google Scholar
Lou, H., Zhu, J.Y., Lan, T.Q., Lai, H., Qiu, X. (2013) pH-Induced Lignin Surface Modification to Reduce Nonspecific Cellulase Binding and Enhance Enzymatic Saccharification of Lignocelluloses. ChemSusChem 6:919–927.10.1002/cssc.201200859Suche in Google Scholar
Mabee, W.E., Gregg, D.J., Arato, C., Berlin, A., Bura, R., Gilkes, N., Mirochnik, O., Pan, X., Pye, E.K., Saddler, J.N. (2006) Updates on softwood-to-ethanol process development. Appl. Biochem. Biotechnol. 129:55–70.10.1007/978-1-59745-268-7_5Suche in Google Scholar
Mansfield, S.D., Mooney, C., Saddler, J.N. (1999) Substrate and enzyme characteristics that limit cellulose hydrolysis. Biotechnol. Prog. 15:804–816.10.1021/bp9900864Suche in Google Scholar
Mendes, C., Teixeira, V., Baptista, C.M.S.G., Rocha, J.M.S., Carvalho, M.G.V.S. (2009) Prehydrolysis of Eucalyptus globulus Labill. hemicelluloses prior to pulping and fermentation of the hydrolysates with the yeast Pichia stipitis 10th EWLP, Stockholm, Sweden, August 25–28, 2008. Holzforschung 63:737–743.10.1515/HF.2009.106Suche in Google Scholar
Muzamal, M., Jedvert, K., Theliander, H., Rasmuson, A. (2015) Structural changes in spruce wood during different steps of steam explosion pretreatment. Holzforschung 69:61–66.10.1515/hf-2013-0234Suche in Google Scholar
Nebreda, A.P., Grénman, H., Mäki-Arvela, P., Eränen, K., Hemming, J., Willför, S., Murzin, D.Y., Salmi, T. (2016) Acid hydrolysis of O-acetyl-galactoglucomannan in a continuous tube reactor: a new approach to sugar monomer production. Holzforschung 70:187–194.10.1515/hf-2014-0314Suche in Google Scholar
Palmqvist, E., Hahn-Hägerdal, B. (2000) Fermentation of lignocellulosic hydrolysates. II: inhibitors and mechanisms of inhibition. Bioresour. Technol. 74:25–33.10.1016/S0960-8524(99)00161-3Suche in Google Scholar
Park, S.J., Um, B.H. (2015) Optimization study on acid hydrolysis of hardwood-derived hemicellulosic extract for alcohol fermentation using response surface methodology. Holzforschung 69:135–141.10.1515/hf-2014-0064Suche in Google Scholar
Perlack, R.D., Wright, L.L., Turhollow, A.F., Graham, R.L., Stokes, B.J., Erbach, D.C. (2005) Biomass as feedstock for a bioenergy and bioproducts industry: the technical feasibility of a billion-ton annual supply. Oak Ridge National Lab, TN.10.2172/1216415Suche in Google Scholar
Pu, Y., Treasure, T., Gonzalez, R., Venditti, R., Jameel, H. (2011) Autohydrolysis pretreatment of mixed hardwoods to extract value prior to combustion. Bioresources 6:4856–4870.10.15376/biores.6.4.4856-4870Suche in Google Scholar
Saska, M., Ozer, E. (1995) Aqueous extraction of sugarcane bagasse hemicellulose and production of xylose syrup. Biotechnol. Bioeng. 45:517–523.10.1002/bit.260450609Suche in Google Scholar PubMed
Schütt, F., Puls, J., Saake, B. (2011) Optimization of steam pretreatment conditions for enzymatic hydrolysis of poplar wood. Holzforschung 65:453–459.10.1515/hf.2011.066Suche in Google Scholar
Sharples, A. (1957) The hydrolysis of cellulose and its relation to structure. Trans. Faraday Soc. 53:1003–1013.10.1039/tf9575301003Suche in Google Scholar
Shuai, L., Yang, Q., Zhu, J.Y., Lu, F.C., Weimer, P.J., Ralph, J., Pan, X.J. (2010) Comparative study of SPORL and dilute-acid pretreatments of spruce for cellulosic ethanol production. Bioresour. Technol. 101:3106–3114.10.1016/j.biortech.2009.12.044Suche in Google Scholar PubMed
Silva, D., A.S., Inoue, H., Endo, T., Yano, S., Bon, E.P.S. (2010) Milling pretreatment of sugarcane bagasse and straw for enzymatic hydrolysis and ethanol fermentation. Bioresour. Technol. 101:7402–7409.10.1016/j.biortech.2010.05.008Suche in Google Scholar PubMed
Sluiter, A., Hames, B., Ruiz, R., Scarlata, C., Sluiter, J., Templeton, D., Crocker, D. (2011) Determination of structural carbohydrates and lignin in biomass. Golden, Colorado: National Renewable Energy Laboratory; 2010 Jul. Report N. TP-510-42618, 17.Suche in Google Scholar
Testova, L., Chong, S.-L., Tenkanen, M., Sixta, H. (2011) Autohydrolysis of birch wood. Holzforschung 65:535–542.10.1515/hf.2011.073Suche in Google Scholar
Tunc, M.S., Chheda, J., van der Heide, E., Morris, J., van Heiningen, A. (2014) Pretreatment of hardwood chips via autohydrolysis supported by acetic and formic acid. Holzforschung 68:401–409.10.1515/hf-2013-0102Suche in Google Scholar
Vila, C., Francisco, J.L., Santos, V., Parajó, J.C. (2013) Effects of hydrothermal processing on the cellulosic fraction of Eucalyptus globulus wood. Holzforschung 67:33–40.10.1515/hf-2012-0046Suche in Google Scholar
Yu, Q., Zhuang, X., Yuan, Z., Wang, Q., Qi, W., Wang, W., Zhang, Y., Xu, J., Xu, H. (2010) Two-step liquid hot water pretreatment of Eucalyptus grandis to enhance sugar recovery and enzymatic digestibility of cellulose. Bioresour. Technol. 101:4895–4899.10.1016/j.biortech.2009.11.051Suche in Google Scholar PubMed
Zhang, Y.-H.P., Lynd, L.R. (2004) Toward an aggregated understanding of enzymatic hydrolysis of cellulose: noncomplexed cellulase systems. Biotechnol. Bioeng. 88:797–824.10.1002/bit.20282Suche in Google Scholar PubMed
Zhang, R., Lu, X., Sun, Y., Wang, X., Zhang, S. (2011) Modeling and optimization of dilute nitric acid hydrolysis on corn stover. J. Chem. Technol. Biotechnol. 86:306–314.10.1002/jctb.2529Suche in Google Scholar
Zhou, H., Zhu, J.Y., Gleisner, R., Qiu, X., Horn, E., Negrón, J. (2016) Pilot-scale demonstration of SPORL for bioconversion of lodgepole pine to bioethanol and lignosulfonate. Holzforschung 70:21–30.10.1515/hf-2014-0332Suche in Google Scholar
Zhu, J.Y., Pan, X.J., Wang, G.S., Gleisner, R. (2009) Sulfite pretreatment (SPORL) for robust enzymatic saccharification of spruce and red pine. Bioresour. Technol. 100:2411–2418.10.1016/j.biortech.2008.10.057Suche in Google Scholar PubMed
Zhu, J.Y., Zhu, W., OBryan, P., Dien, B.S., Tian, S., Gleisner, R., Pan, X.J. (2010) Ethanol production from SPORL-pretreated lodgepole pine: preliminary evaluation of mass balance and process energy efficiency. Appl. Microbiol. Biotechnol. 86:1355–1365.10.1007/s00253-009-2408-7Suche in Google Scholar PubMed
Zhu, J.Y., Zhang, X., Pan, X.J. (2011) Sustainable production of fuels, chemicals, and fibers from forest biomass. ACS Symposium Series (Chapter 9), American Chemical Society, Washington, DC.10.1021/bk-2011-1067Suche in Google Scholar
Zhu, W., Houtman, C.J., Zhu, J.Y., Gleisner, R., Chen, K.F. (2012) Quantitative predictions of bioconversion of aspen by dilute acid and SPORL pretreatments using a unified combined hydrolysis factor (CHF). Process Biochem. 47:785–791.10.1016/j.procbio.2012.02.012Suche in Google Scholar
Zhu, J.Y., Chandra, M.S., Gu, F., Gleisner, R., Reiner, R., Sessions, J., Marrs, G., Gao, J., Anderson, D. (2015) Using sulfite chemistry for robust bioconversion of Douglas-fir forest residue to bioethanol at high titer and lignosulfonate: a pilot-scale evaluation. Bioresour. Technol. 179:390–397.10.1016/j.biortech.2014.12.052Suche in Google Scholar PubMed
©2017 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Original Articles
- Effects of hot water extraction (HWE) of Douglas fir as a pre-process for the sulfite pretreatment to overcome recalcitrance of lignocellulose (SPORL)
- Structural characteristics of milled wood lignin (MWL) isolated from green liquor (GL) pretreated poplar (Populus deltoides)
- TEMPO-mediated electro-oxidation reactions of non-phenolic β-O-4-type lignin model compounds
- Microstructure of chemically modified wood using X-ray computed tomography in relation to wetting properties
- The effects of thermal treatment on the nanomechanical behavior of bamboo (Phyllostachys pubescens Mazel ex H. de Lehaie) cell walls observed by nanoindentation, XRD, and wet chemistry
- Assessing specific gravity of young Eucalyptus plantation trees using a resistance drilling technique
- A novel device to measure gaseous permeability over a wide range of pressures: characterisation of slip flow for Norway spruce, European beech, and wood-based materials
- Stress relaxation of composites made of polypropylene and organo-montmorillonite modified wood flour during water immersion
- Sorption/desorption hysteresis revisited. Sorption properties of Pinus pinea L. analysed by the parallel exponential kinetics and Kelvin-Voigt models
- Effect of exogenous IAA on tension wood formation by facilitating polar auxin transport and cellulose biosynthesis in hybrid poplar (Populus deltoids × Populus nigra) wood
Artikel in diesem Heft
- Frontmatter
- Original Articles
- Effects of hot water extraction (HWE) of Douglas fir as a pre-process for the sulfite pretreatment to overcome recalcitrance of lignocellulose (SPORL)
- Structural characteristics of milled wood lignin (MWL) isolated from green liquor (GL) pretreated poplar (Populus deltoides)
- TEMPO-mediated electro-oxidation reactions of non-phenolic β-O-4-type lignin model compounds
- Microstructure of chemically modified wood using X-ray computed tomography in relation to wetting properties
- The effects of thermal treatment on the nanomechanical behavior of bamboo (Phyllostachys pubescens Mazel ex H. de Lehaie) cell walls observed by nanoindentation, XRD, and wet chemistry
- Assessing specific gravity of young Eucalyptus plantation trees using a resistance drilling technique
- A novel device to measure gaseous permeability over a wide range of pressures: characterisation of slip flow for Norway spruce, European beech, and wood-based materials
- Stress relaxation of composites made of polypropylene and organo-montmorillonite modified wood flour during water immersion
- Sorption/desorption hysteresis revisited. Sorption properties of Pinus pinea L. analysed by the parallel exponential kinetics and Kelvin-Voigt models
- Effect of exogenous IAA on tension wood formation by facilitating polar auxin transport and cellulose biosynthesis in hybrid poplar (Populus deltoids × Populus nigra) wood