Exposure to bleomycin nanoaerosol does not induce fibrosis in mice
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Elena A. Shlyapnikova
Elena A. Shlyapnikova obtained her PhD degree in Chemistry in 1978 at the Moscow State University. She has worked at the Institute of Theoretical and Experimental Biophysics of RAS since 1978. Her present research work focuses on interface chemistry and physics and on the development of microarray-based analytical systems.
Igor L. Kanev obtained his PhD degree in Physical Science in 2014 at the Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences. Since 2010, he has worked as a research fellow at the same Institute. His research work focuses on electrospray technology, generation and dosimetry of nanoaerosolized drugs.
Nadezhda N. Novikova obtained her PhD in Biology from the Institute of Cell Biophysics of RAS in 2000. She is an experienced pathologist in the area of pre-clinical toxicology. The pathological studies which she performs are conducted in accordance with the Good Laboratory Practices. In October 2006 she completed a course in the Good Laboratory Practices Boot Camp conducted at Eli Lilly and Company Lilly, Research Laboratories GLP Quality Assurance Department, Greenfield, IN, USA.
Elena G. Litvinova received her PhD degree in Radiobiology from the Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences in 1985. Her research area includes oxidative stress and antioxidant defense in living organisms as well as the effects of stress on the immune system.
Yuri M. Shlyapnikov obtained his PhD degree in Chemistry in 2010 at the Moscow State University, Russia. In 2006–2009 he worked as a PhD student in the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of RAS. He has worked at the Institute of Theoretical and Experimental Biophysics of RAS since 2010. His research work deals with the development of ultra-sensitive immunochemical and DNA heterophase assays.
Victor N. Morozov obtained PhD and DSc in Biophysics from the Moscow State University. He worked as a visiting scientist at New York University in 1992–1998, in 2002–2004 at the Beckman Research Institute at Temecula, CA, and in 2004–2009 at the National Center for Biodefense and Infectious Diseases of the George Mason University, VA. His studies are focused on the effects of nanoaersolized substances and on development of biomedical nanotechnologies: nanoaerosol generators, methods of collection and detection of airborne biomarkers in exhaled air and in environment.
Abstract
Bleomycin (BLM), a cytostatic drug widely used in the treatment of cancer, often induces lung fibrosis as a side effect. This phenomenon has been exploited in biomedical studies to create a fibrosis model. Inhalation of BLM nanoaerosol particles (NAPs) was expected to induce severe fibrosis in mice. Instead, inhalation of BLM NAPs for 13 days with a total inhaled dose of ~1 mg/kg did not cause pneumofibrosis in CD-1 mice; only marked pneumonitis with infiltration of macrophages in all parts of the lungs was observed in all eight mice exposed. These changes were notably reversible within 2 weeks of cessation of exposure. Despite the limited number of animals it was confidently concluded that slow administration of BLM in the form of NAPs did not cause fibrosis as the bolus instillation of similar doses does.
About the authors
Elena A. Shlyapnikova obtained her PhD degree in Chemistry in 1978 at the Moscow State University. She has worked at the Institute of Theoretical and Experimental Biophysics of RAS since 1978. Her present research work focuses on interface chemistry and physics and on the development of microarray-based analytical systems.
Igor L. Kanev obtained his PhD degree in Physical Science in 2014 at the Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences. Since 2010, he has worked as a research fellow at the same Institute. His research work focuses on electrospray technology, generation and dosimetry of nanoaerosolized drugs.
Nadezhda N. Novikova obtained her PhD in Biology from the Institute of Cell Biophysics of RAS in 2000. She is an experienced pathologist in the area of pre-clinical toxicology. The pathological studies which she performs are conducted in accordance with the Good Laboratory Practices. In October 2006 she completed a course in the Good Laboratory Practices Boot Camp conducted at Eli Lilly and Company Lilly, Research Laboratories GLP Quality Assurance Department, Greenfield, IN, USA.
Elena G. Litvinova received her PhD degree in Radiobiology from the Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences in 1985. Her research area includes oxidative stress and antioxidant defense in living organisms as well as the effects of stress on the immune system.
Yuri M. Shlyapnikov obtained his PhD degree in Chemistry in 2010 at the Moscow State University, Russia. In 2006–2009 he worked as a PhD student in the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of RAS. He has worked at the Institute of Theoretical and Experimental Biophysics of RAS since 2010. His research work deals with the development of ultra-sensitive immunochemical and DNA heterophase assays.
Victor N. Morozov obtained PhD and DSc in Biophysics from the Moscow State University. He worked as a visiting scientist at New York University in 1992–1998, in 2002–2004 at the Beckman Research Institute at Temecula, CA, and in 2004–2009 at the National Center for Biodefense and Infectious Diseases of the George Mason University, VA. His studies are focused on the effects of nanoaersolized substances and on development of biomedical nanotechnologies: nanoaerosol generators, methods of collection and detection of airborne biomarkers in exhaled air and in environment.
Acknowledgments
We acknowledge funding from the Russian Science Foundation (Grant No. 15-15-00086). The authors appreciate fruitful discussions with Andrey Mikheev and Tamara Morozova, members of the Laboratory of Nanostructures and Nanotechnology. The authors also acknowledge the substantial contribution of Dr. Tamara Morozova to the preparation of the manuscript and the assistance of Jennifer Guernsey in editing the manuscript.
Conflict of interest statement: Authors state no conflict of interest. All authors have read the journal’s Publication ethics and publication malpractice statement available at the journal’s website and hereby confirm that they comply with all parts applicable to the present scientific work.
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©2016 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- In this issue
- Guest Editorial
- Nanomaterials for medicine: benefits and risks
- Original Articles
- Synthesis and surface characterization of nanosized Y2O3:Eu and YAG:Eu luminescent phosphors which are useful in photodynamic therapy of cancer
- Benzyldimethyl[3-(miristoylamino)-propyl]ammonium chloride stabilized silver nanoparticles (Argumistin™) in medicine: results of clinical trials for treatment of infectious diseases of dogs and perspectives for humans
- Preparation of electroconductive aluminum-silicon oxide ceramic sensors modified with silver and bismuth nanoparticles
- Exposure of nano- and ultrafine Ni particles to synthetic biological solutions: predicting fate-related dissolution and accumulation
- Regular contributions
- Original Article
- Exposure to bleomycin nanoaerosol does not induce fibrosis in mice
- Short Communications
- The use of objective oriented project planning tools for nanosafety and health concerns: a case study in nanomedicine research project
- Crede’s method in eye water finds a nanomedicine base: a potential candidate to control ophthalmia neonatorum
Artikel in diesem Heft
- Frontmatter
- In this issue
- Guest Editorial
- Nanomaterials for medicine: benefits and risks
- Original Articles
- Synthesis and surface characterization of nanosized Y2O3:Eu and YAG:Eu luminescent phosphors which are useful in photodynamic therapy of cancer
- Benzyldimethyl[3-(miristoylamino)-propyl]ammonium chloride stabilized silver nanoparticles (Argumistin™) in medicine: results of clinical trials for treatment of infectious diseases of dogs and perspectives for humans
- Preparation of electroconductive aluminum-silicon oxide ceramic sensors modified with silver and bismuth nanoparticles
- Exposure of nano- and ultrafine Ni particles to synthetic biological solutions: predicting fate-related dissolution and accumulation
- Regular contributions
- Original Article
- Exposure to bleomycin nanoaerosol does not induce fibrosis in mice
- Short Communications
- The use of objective oriented project planning tools for nanosafety and health concerns: a case study in nanomedicine research project
- Crede’s method in eye water finds a nanomedicine base: a potential candidate to control ophthalmia neonatorum
