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Towards nano-diagnostics for rapid diagnosis of infectious diseases – current technological state

  • Georgette B. Salieb-Beugelaar

    Georgette B. Salieb-Beugelaar began her professional life in the fields of clinical genetics studying DNA research and diagnostics at the Academic Medical Centre in Amsterdam (The Netherlands) in 1996. She studied in parallel Chemistry at the University of Utrecht (The Netherlands) between 2000 and 2003. In 2005, Georgette changed her professional field into the microfluidic and nanofluidic world at the University of Twente in Enschede (The Netherlands) and investigated single DNA molecules in nanoconfined environments on chips. For this she received my PhD in 2009, and continued the next 2 years at the Korean Institute of Science and Technology (Saarbrücken, Germany) with nanodropled pseudocrystals in microfluidic chips. Meanwhile, she was also working for the Mesa Institute for Nanotechnology (Twente University), which was then setting up their BioNano Laboratory. Since November 2012, Georgette has been a member of the multidisciplinary NanoMedicine Group of Prof. Patrick Hunziker, and works for the DiscoGnosis project (www.discognosis.eu). She is involved in this journal as an assistant communicating and managing editor.

     EMAIL logo     and Patrick R. Hunziker

    Patrick Hunziker studied Medicine at the University of Zurich, Switzerland. He received a doctoral degree based on thesis work in Experimental Immunology from the University of Zurich and did further research in Experimental Hematology at University Hospital in Zurich, Switzerland. He earned specialist degrees in Internal Medicine, Cardiology and Intensive Care Medicine. As a fellow of the Massachusetts General Hospital, Harvard Medical School, he worked on cardiac imaging in a joint project with the Massachusetts Institute of Technology, Cambridge. His professional activities in Europe, the USA, Africa and China gave him a broad insight into the needs for the medicine of the future in a variety of settings. Patrick Hunziker became involved in medical applications of nanoscience in the late 1990s and has been the pioneer physician in nanomedicine in Switzerland since then. With improved prevention, diagnosis and cure of cardiovascular disease as his main research topic, he worked in the nanoscience fields of atomic force microscopy, nano-optics, micro/nanofluidics, nanomechanical sensors and polymer nanocarriers for targeting. He is the founding president of the European Society of Nanomedicine, cofounder of the European Foundation for Clinical Nanomedicine and coinitiator of the European Conference for Clinical Nanomedicine and is clinically active as deputy head of the Clinic for Intensive Care Medicine at the University Hospital Basel, Switzerland. In November 2008 he became Professor for Cardiology and Intensive Care Medicine at the University of Basel.
Published/Copyright: March 6, 2014
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European Journal of Nanomedicine
From the journal European Journal of Nanomedicine Volume 6 Issue 1

Abstract

Rapid, specific and sensitive diagnosis of infectious diseases is important for the rational, effective and economic treatment of the infection. Focused treatment of the patient with an accurate diagnosis may also contribute to the minimization of drug resistance development. Rapidly evolving micro and nanotechnologies contribute to the development of new diagnostic modalities. This critical review provides an overview of current developments in diagnostics for infectious disease enabled by nanotechnologies and includes a range of representative examples.

Keywords: lateral flow devices; microfluidics; nano-diagnostics; nanotechnology; nanoparticles; point-of-care
Corresponding author: Georgette B. Salieb-Beugelaar, Medizinische Intensiv-Pflegestation (MIPS), Research Group Nanomedicine, Universitätsspital Basel, Petersgraben 4, CH-4031 Basel, Switzerland; and The European Foundation for Clinical Nanomedicine (CLINAM), Alemannengasse 12, CH-4016 Basel, Switzerland, E-mail:

About the authors

Georgette B. Salieb-Beugelaar

Georgette B. Salieb-Beugelaar began her professional life in the fields of clinical genetics studying DNA research and diagnostics at the Academic Medical Centre in Amsterdam (The Netherlands) in 1996. She studied in parallel Chemistry at the University of Utrecht (The Netherlands) between 2000 and 2003. In 2005, Georgette changed her professional field into the microfluidic and nanofluidic world at the University of Twente in Enschede (The Netherlands) and investigated single DNA molecules in nanoconfined environments on chips. For this she received my PhD in 2009, and continued the next 2 years at the Korean Institute of Science and Technology (Saarbrücken, Germany) with nanodropled pseudocrystals in microfluidic chips. Meanwhile, she was also working for the Mesa Institute for Nanotechnology (Twente University), which was then setting up their BioNano Laboratory. Since November 2012, Georgette has been a member of the multidisciplinary NanoMedicine Group of Prof. Patrick Hunziker, and works for the DiscoGnosis project (www.discognosis.eu). She is involved in this journal as an assistant communicating and managing editor.

Patrick R. Hunziker

Patrick Hunziker studied Medicine at the University of Zurich, Switzerland. He received a doctoral degree based on thesis work in Experimental Immunology from the University of Zurich and did further research in Experimental Hematology at University Hospital in Zurich, Switzerland. He earned specialist degrees in Internal Medicine, Cardiology and Intensive Care Medicine. As a fellow of the Massachusetts General Hospital, Harvard Medical School, he worked on cardiac imaging in a joint project with the Massachusetts Institute of Technology, Cambridge. His professional activities in Europe, the USA, Africa and China gave him a broad insight into the needs for the medicine of the future in a variety of settings. Patrick Hunziker became involved in medical applications of nanoscience in the late 1990s and has been the pioneer physician in nanomedicine in Switzerland since then. With improved prevention, diagnosis and cure of cardiovascular disease as his main research topic, he worked in the nanoscience fields of atomic force microscopy, nano-optics, micro/nanofluidics, nanomechanical sensors and polymer nanocarriers for targeting. He is the founding president of the European Society of Nanomedicine, cofounder of the European Foundation for Clinical Nanomedicine and coinitiator of the European Conference for Clinical Nanomedicine and is clinically active as deputy head of the Clinic for Intensive Care Medicine at the University Hospital Basel, Switzerland. In November 2008 he became Professor for Cardiology and Intensive Care Medicine at the University of Basel.

Acknowledgments

This review has been written to contribute to the DiscoGnosis project that has the core objective to develop a platform that would allow the detection of malaria and similar pathogenic diseases in a rapid, multiplexed and non-invasive way (www.discognosis.eu). This project is supported by the European Commission through the 7th Framework Programme on Research and Technological Development within the Objective FP7 ICT-2011.3.2 and under Grant Agreement No. 318408.

References

1. Neumann G, Noda T, Kawaoka Y. Emergence and pandemic potential of swine-origin H1N1 influenza virus. Nature 2009;459:931–9.10.1038/nature08157Search in Google Scholar PubMed PubMed Central

2. Desai D, Wu G, Zaman MH. Tackling HIV through robust diagnostics in the developing world: current status and future opportunities. Lab Chip 2011;11:194–211.10.1039/C0LC00340ASearch in Google Scholar PubMed PubMed Central

3. Hauck TS, Giri S, Gao Y, Chan WC. Nanotechnology diagnostics for infectious diseases prevalent in developing countries. Adv Drug Deliver Rev 2010;62:438–48.10.1016/j.addr.2009.11.015Search in Google Scholar PubMed

4. Diamandis PE, Christopoulos TK. Immunoassay. Academic Press. ISBN-10: 0122147308, ISBN-13: 978-0122147302.Search in Google Scholar

5. Wong R, Tse H. Lateral Flow Immunoassay. USA, NY: Humana Press, ISBN: 978-1-58829-908-6.Search in Google Scholar

6. Mak WC, Sin KK, Chan CP, Wong LW, Renneberg R. Biofunctionalized indigo-nanoparticles as biolabels for the generation of precipitated visible signal in immunodipsticks. Biosensors Bioelectronics 2011;26:3148–53.10.1016/j.bios.2010.12.016Search in Google Scholar PubMed

7. Mashayekhi F, Le AM, Nafisi PM, Wu BM, Kamei DT. Enhancing the lateral-flow immunoassay for detection of proteins using an aqueous two-phase micellar system. Anal Bioanal Chem 2012;404:2057–66.10.1007/s00216-012-6278-ySearch in Google Scholar PubMed

8. Puertas S, Moros M, Fernández-Pacheco R, Ibarra MR, Grazú V, de la Fuente JM. Designing novel nano-immunoassays: antibody orientation versus sensitivity. J Phys D: Appl Phys 2010;43:474012.10.1088/0022-3727/43/47/474012Search in Google Scholar

9. Salieb-Beugelaar GB, Simone G, Arora A, Philippi A, Manz A. Latest developments in microfluidic cell biology and analysis systems. Anal Chem 2010;82:4848–64.10.1021/ac1009707Search in Google Scholar PubMed

10. Arora A, Simone G, Salieb-Beugelaar GB, Kim JT, Manz A. Latest developments in micro total analysis systems. Anal Chem 2010;82:4830–47.10.1021/ac100969kSearch in Google Scholar PubMed

11. Lim YC, Kouzani AZ, Duan W. Lab-on-a-chip: a component view. Microsyst Technol 2010;16:1995–2015.10.1007/s00542-010-1141-6Search in Google Scholar

12. Nge PN, Rogers CI, Woolley AT. Advances in microfluidic materials, functions, integration, and applications. Chem Rev 2013;113:2550–83.10.1021/cr300337xSearch in Google Scholar PubMed PubMed Central

13. Salieb-Beugelaar GB, Teapal J, van Nieuwkasteele J, Wijnperlé D, Tegenfeldt JO, Lisdat F, et al. Field-dependent DNA mobility in 20 nm High nanoslits. Nano Lett 2008;8:1785–90.10.1021/nl080300vSearch in Google Scholar PubMed

14. Xu ZR, Zhong CH, Guan YX, Chen XW, Wang JH, Fang ZL. A microfluidic flow injection system for DNA assay with fluids driven by an on-chip integrated pump based on capillary and evaporation effects. Lab Chip 2008;8:1658–63.10.1039/b805774eSearch in Google Scholar PubMed

15. Zimmermann M, Schmid H, Hunziker P, Delamarche E. Capillary pumps for autonomous capillary systems. Lab Chip 2007;7: 119–25.10.1039/B609813DSearch in Google Scholar

16. Sato T, Kawai K, Kanai M, Shoji S. Development of an all-fluoroplastic microfluidic device applied as a nanoliter sample injector Jpn. J Appl Phys 2009;48:06FJ03.10.1143/JJAP.48.06FJ03Search in Google Scholar

17. Scott DE, Grigsby RJ, Lunt SM. Microdialysis sampling coupled to microchip electrophoresis with integrated amperometric detection on an all-glass substrate. ChemPhysChem 2013;14:2288–94.10.1002/cphc.201300449Search in Google Scholar PubMed PubMed Central

18. Lee J, Lee SH. Lab on a chip for in situ diagnosis: from blood to point of care. Biomed Eng Lett 2013;3:59–66.10.1007/s13534-013-0094-ySearch in Google Scholar PubMed PubMed Central

19. Cui J, Pan T. A vacuum-driven peristaltic micropump with valved actuation chambers. J Micromech Microeng 2011;21:065034.10.1088/0960-1317/21/6/065034Search in Google Scholar

20. Yang YN, Hsiun SK, Lee GB. A pneumatic micropump incorporated with a normally closed valve capable of generating a high pumping rate and a high back pressure. Microfluid Nanofluid 2009;6:823–33.10.1007/s10404-008-0356-7Search in Google Scholar

21. Zimmermann M, Hunziker P, Delamarche E. Valves for autonomous capillary systems. Biomed Microdevices 2009;11:1–8.10.1007/s10544-008-9187-2Search in Google Scholar PubMed

22. Godino N, Comaskey E, Gorkin III R, Ducré J. Centrifugally enhanced paper microfluidics. 978-1-4673-0325-5/12, MEMS 2012, Paris, FRANCE, 29 January–2 February 2012.Search in Google Scholar

23. Hunag CC, Bazant MZ, Thorsen T. Ultrafast high-pressure AC electro-osmotic pumps for portable biomedical microfluidics. Lab Chip 2010;10:80–5.10.1039/B915979GSearch in Google Scholar PubMed

24. Lim CY, Lam YC, Yang C. Mixing enhancement in microfluidic channel with a constriction under periodic electro-osmotic flow. Biomicrofluidics 2010;4:14101.10.1063/1.3279790Search in Google Scholar PubMed PubMed Central

25. Liu RH, Lenigk R, Druyor-Sanchez RL, Yang J, Grodzinski P. Hybridization enhancement using cavitation microstreaming. Anal Chem 2003;75:1911–7.10.1021/ac026267tSearch in Google Scholar PubMed

26. El Moctar AO, Aubry N, Batton J. Electro-hydrodynamic micro-fluidic mixer. Lab Chip 2003;3:273–80.10.1039/b306868bSearch in Google Scholar PubMed

27. Lee CY, Chang CL, Wang YN, Fu LM. Microfluidic mixing: a review. Int J Mol Sci 2011;12:3263–87.10.3390/ijms12053263Search in Google Scholar PubMed PubMed Central

28. Grünberger A, Paczia N, Probst C, Schendzielorz G, Eggeling L, Noack S, et al. A disposable picolitre bioreactor for cultivation and investigation of industrially relevant bacteria on the single cell level. Lab Chip 2012;12:2060–8.10.1039/c2lc40156hSearch in Google Scholar PubMed

29. Freifeld AG, Simonsen KA, Booth CS, Zhao X, Whitneu SE, Karre T, et al. A new rapid method for clostridium difficile DNA extraction and detection in stool. Toward point-of-care diagnostic testing. Journal of Molecular Diagnostics 2012;14:274–9.10.1016/j.jmoldx.2012.01.003Search in Google Scholar PubMed

30. Asanomi Y, Yamaguchi H, Miyazaki M, Maeda H. Enzyme-immobilized microfluidic process reactors. Molecules 2011;16:6041–59.10.3390/molecules16076041Search in Google Scholar PubMed PubMed Central

31. Wolf M, Juncker D, Michel B, Hunziker P, Delamarche E. Simultaneous detection of C-reactive protein and other cardiac markers in human plasma using micromosaic immunoassays and self-regulating microfluidic networks. Biosens Bioelectron 2004;19:1193–202.10.1016/j.bios.2003.11.003Search in Google Scholar PubMed

32. Henley SJ, Beliatis MJ, Stolojan V, Ravi S, Silva P. Laser implantation of plasmonic nanostructures into glass. Nanoscale 2013;5:1054–9.10.1039/C2NR33629DSearch in Google Scholar PubMed

33. Helmerhorst E, Chandler DJ, Nussio M, Mamotte CD. Real-time and label-free bio-sensing of molecular interactions by surface plasmon resonance: a laboratory medicine perspective. Clin Biochem Rev 2012;33:161–73.Search in Google Scholar

34. de Souza FR, Alves GL, Coltro WK. Capillary-driven toner-based microfluidic devices for clinical diagnostics with colorimetric detection. Anal Chem 2012;84:9002–7.10.1021/ac302506kSearch in Google Scholar PubMed

35. Zhou G, Mao X, Juncker D. Immunochromatographic assay on thread. Anal Chem 2012;84:7736–43.10.1021/ac301082dSearch in Google Scholar PubMed

36. Zimmermann M, Delamarche E, Wolf M, Hunziker P. Modeling and optimization of high-sensitivity, low-volume microfluidic-based surface immunoassays. Biomed Microdevices 2005;7:99–110.10.1007/s10544-005-1587-ySearch in Google Scholar PubMed

37. Sabotin I, Tristo G, Junkar M, Valentinčič J. Two-step design protocol for patterned groove micromixers. Chem Eng Res Design 2013;91:778–88.10.1016/j.cherd.2012.09.013Search in Google Scholar

38. Bai B, Luo Z, Lu T, Xu F. Numerical simulation of cell adhesion and detachment in microfluidics. J Mech Med Biol 2013;13:1350002.10.1142/S0219519413500024Search in Google Scholar

39. Yuan Q, Yang K, Wu J. Optimization of planar interdigitated microelectrode array for biofluid transport by AC electrothermal effect. Microfluid Nanofluid 2014;16:167–78.10.1007/s10404-013-1231-8Search in Google Scholar

40. Yang Z, Zong C, Ju H, Yan F. Streptavidin-functionalized capillary immune microreactor for highly efficient chemiluminescent immunoassay. Anal Chim Acta 2011;706:143–8.10.1016/j.aca.2011.08.011Search in Google Scholar PubMed

41. White RJ, Kallewaard HM, Hsieh W, Patterson AS, Kasehagen JB, Cash KJ, et al. A Wash-free, electrochemical platform for the quantitative, multiplexed detection of specific antibodies. Anal Chem 2012; 84:1098–103.10.1021/ac202757cSearch in Google Scholar PubMed PubMed Central

42. Focke M, Kosse D, Müller C, Reinecke H, Zengerle R, von Stetten F. Lab-on-a-Foil: microfluidics on thin and flexible films. Lab Chip 2010;10:1365–86.10.1039/c001195aSearch in Google Scholar PubMed

43. Sharma H, Nguyen D, Chen A, Lew V, Khine M. Unconventional low-cost fabrication and patterning techniques for point of care diagnostics. Ann Biomed Eng 2011;39:1313–27.10.1007/s10439-010-0213-1Search in Google Scholar PubMed PubMed Central

44. Lafleur L, Stevens D, McKenzie K, Ramachandran S, Spicar-Mihalic P, Singhal M, et al. Progress toward multiplexed sample-to-result detection in low resource settings using microfluidic immunoassay cards. Lab Chip 2012;12:1119–27.10.1039/c2lc20751fSearch in Google Scholar PubMed

45. Yu X, Xia HS, Sun ZD, Lin Y, Wang K, Yu J, et al. On-chip dual detection of cancer biomarkers directly in serum based on self-assembled magnetic bead patterns and quantum dots. Biosens Bioelectron 2013;41:129–36.10.1016/j.bios.2012.08.007Search in Google Scholar PubMed

46. Zheng C, Wang X, Lu Y, Liu Y. Rapid detection of fish major allergen parvalbumin using superparamagnetic nanoparticle-based lateral flow immunoassay. Food Control 2012;26:446–52.10.1016/j.foodcont.2012.01.040Search in Google Scholar

47. Wang B, Weldon AL, Kumnorkaew P, Xu B, Gilchrist JF, Cheng X. Effect of surface nanotopography on immunoaffinity cell capture in microfluidic devices. Langmuir 2011;27:11229–37.10.1021/la2015868Search in Google Scholar PubMed

48. Serrate D, De Teresa JM, Marquina C, Marzo J, Saurel D, Cardoso FA, et al. Quantitative biomolecular sensing station based on magnetoresistive patterned arrays. Biosens Bioelectron 2012;35:206–12.10.1016/j.bios.2012.02.048Search in Google Scholar PubMed

49. Marquina C, de Teresa JM, Serrate D, Marzo J, Cardoso FA, Saurel D, et al. GMR sensors and magnetic nanoparticles for immuno-chromatographic assays. Biosens Bioelectron. J Mag and Mag Mat 2012;324:3495–8.10.1016/j.jmmm.2012.02.074Search in Google Scholar

50. Cardoso FA, Germano J, Ferreira R, Cardoso S, Martins VC, Freitas PP, et al. Detection of 130nm magnetic particles by a portable electronic platform using spin valve and magnetic tunnel junction sensors. J App Phys 2008;103:07A310.10.1063/1.2836713Search in Google Scholar

51. Zhou F, Yuan L, Wang H, Li D, Chen H. Gold nanoparticle layer: a promising platform for ultra-sensitive cancer detection. Langmuir 2011;27:2155–8.10.1021/la1049937Search in Google Scholar PubMed

52. Zhou F, Wang M, Yuan L, Cheng Z, Wu Z, Chen H. Sensitive sandwich ELISA based on a gold nanoparticle layer for cancer detection. Analyst 2012;137:1779–84.10.1039/c2an16257aSearch in Google Scholar PubMed

53. Juntunen E, Myyryläinen T, Salminen T, Soukka T, Pettersson K. Performance of fluorescent europium(III) nanoparticles and colloidal gold reporters in lateral flow bioaffinity assay. Anal Biochem 2012;428:31–8.10.1016/j.ab.2012.06.005Search in Google Scholar PubMed

54. Zong S, Wang Z, Zhang R, Wang C, Xu S, Cui Y. A multiplex and straightforward aqueous phase immunoassay protocol through the combination of SERS-fluorescence dual mode nanoprobes and magnetic nanobeads. Biosens Bioelectron 2013;41:745–51.10.1016/j.bios.2012.09.057Search in Google Scholar PubMed

55. Noguera P, Posthuma-Trumpie GA, van Tuil M, van der Wal FJ, de Boer A, Moers AP, et al. Carbon nanoparticles in lateral flow methods to detect genes encoding virulence factors of Shiga toxin-producing Escherichia coli. Anal Bioanal Chem 2011;399:831–8.10.1007/s00216-010-4334-zSearch in Google Scholar PubMed PubMed Central

56. Posthuma-Trumpie GA, Wichers JH, Koets M, Berendsen LB, van Amerongen A. Amorphous carbon nanoparticles: a versatile label for rapid diagnostic (immuno)assays. Anal Bioanal Chem 2012;402:593–600.10.1007/s00216-011-5340-5Search in Google Scholar PubMed PubMed Central

57. Goryacheva IY, Lenain P, de Saeger S. Nanosized labels for rapid immunotests. Trends Anal Chem 2013;46:30–43.10.1016/j.trac.2013.01.013Search in Google Scholar

58. Chai C, Takhistov P. Label-freetoxin detection by meansof time-resolved electrochemical impedance spectroscopy. Sensors (Basel) 2010;10:655–69.10.3390/s100100655Search in Google Scholar PubMed PubMed Central

59. Prasad S, Selvam AP, Reddy RK, Love A. Silicon nanosensor for diagnosis of cardiovascular proteomic markers. J Lab Autom 2013;18:143–51.10.1177/2211068212460038Search in Google Scholar PubMed

60. Cheng MS, Ho JS, Tan CH, Wong JP, Ng LC, Toh CS. Development of an electrochemical membrane-based nanobiosensor for ultrasensitive detection of dengue virus. Anal Chim Acta 2012;725:74–80.10.1016/j.aca.2012.03.017Search in Google Scholar PubMed

61. Nguyen BT, Peh AE, Chee CY, Fink K, Chow VT, Ng MM, et al. Electrochemical impedance spectroscopy characterization of nanoporous alumina dengue virus biosensor. ioelectrochemistry 2012;88:15–21.10.1016/j.bioelechem.2012.04.006Search in Google Scholar PubMed

62. Peh AE, Li SF. Dengue virus detection using impedance measured across nanoporous alumina membrane. Biosens Bioelectron 2013;42:391–6.10.1016/j.bios.2012.10.054Search in Google Scholar PubMed

63. Wang YC, Stevens AL, Han J. Million-fold preconcentration of proteins and peptides by nanofluidic filter. Anal Chem 2005;77:4293–9.10.1021/ac050321zSearch in Google Scholar PubMed

64. Wu D, Steckl A. High speed nanofluidic protein accumulator. Lab Chip 2009;9:1890–6.10.1039/b823409dSearch in Google Scholar PubMed

65. Cui Y, Wei Q, Park H, Lieber CM. Nanowire nanosensors for highly sensitive and selective detection of biological and chemical species. Science 2001;293:1289–92.10.1126/science.1062711Search in Google Scholar PubMed

66. Patolsky F, Zheng G, Hayden O, Lakadamyali M, Zhuang X, Lieber CM. Electrical detection of single viruses. Proc Natl Acad Sci USA 2004;101:14017–22.10.1073/pnas.0406159101Search in Google Scholar PubMed PubMed Central

67. Tarasov A, Wipf M, Bedner K, Kurz J, Fu W, Guzenko VA, et al. True reference nanosensor realized with silicon nanowires. Langmuir 2012;28:9899–905.10.1021/la301555rSearch in Google Scholar PubMed

68. Gong JR. Label-free attomolar detection of proteins using integrated nanoelectronic and electrokinetic devices. Small 2010;6:967–73.10.1002/smll.200902132Search in Google Scholar PubMed

69. Abonnenc M, Manaresi N, Borgatti M, Medoro G, Fabbri E, Romani A, et al. Programmable interactions of functionalized single bioparticles in a dielectrophoresis-based microarray chip. Anal Chem 2013;85:8219–24.10.1021/ac401296mSearch in Google Scholar PubMed

70. Hansen KM, Ji HF, Wu G, Datar R, Cote R, Majumdar A, et al. Cantilever-based optical deflection assay for discrimination of dna single-nucleotide mismatches. Anal Chem 2001;73: 1567–71.10.1021/ac0012748Search in Google Scholar PubMed

71. Wu G, Datar RH, Hansen KM, Thundat T, Cote RJ, Majumdar A. Bioassay of prostate-specific antigen (PSA) using microcantilevers. Nat Biotechnol 2001;19:856–60.10.1038/nbt0901-856Search in Google Scholar PubMed

72. Arntz Y, Seelig JD, Lang HP, Zhang J, Hunziker P, Ramseuer JP, et al. Label-free protein assay based on a nanomechanical cantilever array. Nanotechnology 2003;14:86–90.10.1088/0957-4484/14/1/319Search in Google Scholar

73. Roy D, Kwon SH, Kwak JW, Park JW. “Seeing and Counting” individual antigens captured on a microarrayed spot with force-based atomic force microscopy. Anal Chem 2010;82: 5189–94.10.1021/ac100476bSearch in Google Scholar PubMed

74. Gfeller KY, Nugaeva N, Hegner M. Rapid biosensor for detection of antibiotic-selective growth of Escherichia coli. Appl Environ Microbiol 2005;71:2626–31.10.1128/AEM.71.5.2626-2631.2005Search in Google Scholar PubMed PubMed Central

75. Park K, Jang J, Irimia D, Sturgis J, Lee J, Robinson JP, et al. ‘Living cantilever arrays’ for characterization of mass of single live cells in fluids. Lab Chip 2008;8:1034–41.10.1039/b803601bSearch in Google Scholar PubMed PubMed Central

76. Burg TP, Godin M, Knudsen SM, Shen W, Carlson G, Foster JS, et al. Weighing of biomolecules, single cells and single nanoparticles in fluid. Nature 2007;446:1066–9.10.1038/nature05741Search in Google Scholar PubMed

77. Schmid D, Lang H, Marsch S, Gerber C, Hunziker P. Clinical studies: diagnosing disease by nanomechanical olfactory sensors–system design and clinical validation. Eur J Nanomed 2008;1:44–7.10.1515/EJNM.2008.1.1.44Search in Google Scholar

78. Frits J. Cantilever biosensors. Analyst 2008;133:855–63.10.1039/b718174dSearch in Google Scholar PubMed

79. Johnson BN, Mutharasan R. Biosensing using dynamic-mode cantilever sensors: a review. Biosens Bioelectron 2012;32:1–18.10.1016/j.bios.2011.10.054Search in Google Scholar PubMed

80. Sukhanova A, Even-Desrumeaux K, Kisserli A, Tabary T, Reveil B, Millot JM, et al. Oriented conjugates of single-domain antibodies and quantum dots: toward a new generation of ultrasmall diagnostic nanoprobes. Nanomedicine 2012;8: 516–25.10.1016/j.nano.2011.07.007Search in Google Scholar PubMed

81. Wesolowski J, Alzogaray V, Reyelt J, Unger M, Juarez K, Urrutia M, et al. Single domain antibodies: promising experimental and therapeutic tools in infection and immunity. Med Microbiol Immunol 2009;198:157–74.10.1007/s00430-009-0116-7Search in Google Scholar PubMed PubMed Central

82. Kim HJ, McCoy MR, Majkova Z, Dechant JE, Gee SJ, Tabares-da Rosa S, et al. Isolation of alpaca anti-hapten heavy chain single domain antibodies for development of sensitive immunoassay. Anal Chem 2012;84:1165–71.10.1021/ac2030255Search in Google Scholar PubMed PubMed Central

83. Han JH, Kim HJ, Sudheendra L, Gee SJ, Hammock BD, Kennedy IM. Photonic crystal lab-on-a chip for detecting staphylococcal enterotoxin b at low attomolar concentration. Anal Chem 2013;85:3104–9.10.1021/ac303016hSearch in Google Scholar PubMed PubMed Central

84. Maraldo D, Mutharasan R. Detection and confirmation of staphylococcal enterotoxin b in apple juice and milk using piezoelectric-excited millimeter-sized cantilever sensors at 2.5 fg/mL. Anal Chem 2007; 79:7636–43.10.1021/ac070589lSearch in Google Scholar PubMed

85. Lee JH, Seo HS, Song JA, Kwon KC, Lee EJ, Kim HJ, et al. Proteinticle engineering for accurate 3D diagnosis. ACS Nano 2013;7:10879–86.10.1021/nn404325tSearch in Google Scholar PubMed

Received: 2014-2-9
Accepted: 2014-2-14
Published Online: 2014-03-06
Published in Print: 2014-03-01

©2014 by Walter de Gruyter Berlin/Boston

Articles in the same Issue

  1. Cover and Frontmatter
  2. Notice from the Editorial Office
  3. News from the European Foundation for Nanomedicine (CLINAM)
  4. What’s up in nanomedicine?
  5. Introducing our Cover Contest Winner
  6. Living immune cells as drug carriers in cancer therapy
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https://doi.org/10.1515/ejnm-2014-0004
Keywords for this article
lateral flow devices; microfluidics; nano-diagnostics; nanotechnology; nanoparticles; point-of-care

Articles in the same Issue

  1. Cover and Frontmatter
  2. Notice from the Editorial Office
  3. News from the European Foundation for Nanomedicine (CLINAM)
  4. What’s up in nanomedicine?
  5. Introducing our Cover Contest Winner
  6. Living immune cells as drug carriers in cancer therapy
  7. Critical Review
  8. Towards nano-diagnostics for rapid diagnosis of infectious diseases – current technological state
  9. Original Articles
  10. Measurement of the size and charge distribution of sodium chloride particles generated by an Aeroneb Pro® pharmaceutical nebulizer
  11. Tailoring surface nanostructures on polyaryletherketones for load-bearing implants
  12. Opinion paper: Meeting report
  13. Nanomedicine: visions, risk, potential
  14. Corrigendum
  15. Nanoemulsions: increasing possibilities in drug delivery
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