Gene-gene interaction of μ-opioid receptor and GluR5 kainate receptor subunit is associated with smoking behavior in a Greek population: presence of a dose allele effect
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Georgios Misailidis
Abstract
Background: Components of nicotine reward system can potentially influence smoking behavior. The μ-opioid receptor (OPRM1) binds the endogenous opioid peptide β-endorphin and mediates the reinforcing effects of nicotine, while the GluR5 kainate receptor subunit (encoded by GRIK1 gene), a binding site for known mediators of glutamate neurotransmission, potentially affects the glutaminergic system that is also indirectly implicated in the reward system.
Methods: In the present study, OPRM1 A118G and GRIK1 rs2832407C>A polymorphisms and their interactions were analyzed in 132 smoking initiators (SI) and 144 non-initiators (NI) of Greek origin, using the PCR-RFLP method.
Results: No differences were found in the genotype or allele distribution of OPRM1 A118G and GRIK1 rs2832407C>A between SI and NI. However, we found a significant interaction of OPRM1 A118G and GRIK1 rs2832407C>A genotypes associated with smoking initiation in a model adjusted for age, sex, BMI and type 2 diabetes mellitus (odds ratio=1.341, 95% CI 1.024–1.755, p=0.033). A dose effect of OPRM1 and GRIK1 variant alleles was present. Increased number of variant alleles (from 0 to 4) was associated with smoking initiation in the same adjusted model (odds ratio=1.537, 95% CI 1.030–2.293, p=0.036).
Conclusions: Smoking phenotype is a complex interaction of genetic and environmental factors. In the present study, we have shown that gene-gene interaction of components of different systems associated with nicotine reinforcing effects, such as OPRM1 and GRIK1, rather than one gene polymorphism, is associated with smoking behavior.
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©2015 by De Gruyter
Artikel in diesem Heft
- Frontmatter
- Editorial
- Population pharmacogenetics and global health
- Review
- Clinical prospects of IGF-signaling system components study in ovarian cancer patients
- Reviews in Population Pharmacogenomics
- Pharmacogenetics of drug-metabolizing enzymes in US Hispanics
- Pharmacogenetics of drug-metabolizing enzymes in Italian populations
- Opinion Paper
- Is laboratory medicine ready for the era of personalized medicine? A survey addressed to laboratory directors of hospitals/academic schools of medicine in Europe
- Original Articles
- Gene-gene interaction of μ-opioid receptor and GluR5 kainate receptor subunit is associated with smoking behavior in a Greek population: presence of a dose allele effect
- In vitro assessment of CYP1A2 and 2C9 inhibition potential of Withania somnifera and Centella asiatica in human liver microsomes
Artikel in diesem Heft
- Frontmatter
- Editorial
- Population pharmacogenetics and global health
- Review
- Clinical prospects of IGF-signaling system components study in ovarian cancer patients
- Reviews in Population Pharmacogenomics
- Pharmacogenetics of drug-metabolizing enzymes in US Hispanics
- Pharmacogenetics of drug-metabolizing enzymes in Italian populations
- Opinion Paper
- Is laboratory medicine ready for the era of personalized medicine? A survey addressed to laboratory directors of hospitals/academic schools of medicine in Europe
- Original Articles
- Gene-gene interaction of μ-opioid receptor and GluR5 kainate receptor subunit is associated with smoking behavior in a Greek population: presence of a dose allele effect
- In vitro assessment of CYP1A2 and 2C9 inhibition potential of Withania somnifera and Centella asiatica in human liver microsomes