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Mechanical strain affects some microRNA profiles in pre-oeteoblasts.

  • Yang Wang , Xianqiong Zou , Yong Guo EMAIL logo , Lu Wang , Yongming Liu , Qiangcheng Zeng and Xizheng Zhang
Published/Copyright: October 15, 2015
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Cellular and Molecular Biology Letters
From the journal Cellular and Molecular Biology Letters Volume 20 Issue 4

Abstract

MicroRNAs (miRNAs) are important regulators of cell proliferation, differentiation and function. Mechanical strain is an essential factor for osteoblast proliferation and differentiation. A previous study revealed that a physiological mechanical tensile strain of 2500 microstrain (με) at 0.5 Hz applied once a day for 1 h over 3 consecutive days promoted osteoblast differentiation. However, the mechanoresponsive miRNAs of these osteoblasts were not identified. In this study, we applied the same mechanical tensile strain to in vitro cultivated mouse MC3T3-E1 pre-osteoblasts and identified the mechanoresponsive miRNAs. Using miRNA microarray and qRT-PCR assays, the expression patterns of miRNAs were evaluated and 5 of them were found to be significantly different between the mechanical loading group and the control group: miR-3077-5p, 3090-5p and 3103-5p were significantly upregulated and miR-466i-3p and 466h-3p were downregulated. Bioinformatics analysis revealed possible target genes for these differentially expressed miRNAs. Some target genes correlated with osteoblast differentiation. These findings indicated that the mechanical strain changed the expression levels of these miRNAs. This might be a potential regulator of osteoblast differentiation and responses to mechanical strain.

Keywords: Mechanical strain; 2500 microstrain (με); Mouse MC3T3-E1 preosteoblasts; MicroRNA; Osteoblast differentiation; Microarray; qRT-PCR; Bioinformatics; Regulator; Mechanoresponsive

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Received: 2014-12-31
Accepted: 2015-6-28
Published Online: 2015-10-15
Published in Print: 2015-12-1

© 2015

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https://doi.org/10.1515/cmble-2015-0034
Keywords for this article
Mechanical strain; 2500 microstrain (με); Mouse MC3T3-E1 preosteoblasts; MicroRNA; Osteoblast differentiation; Microarray; qRT-PCR; Bioinformatics; Regulator; Mechanoresponsive

Articles in the same Issue

  1. Elevated pressure enhanced TRAIL-induced apoptosis in hepatocellular carcinoma cells via ERK1/2-inactivation
  2. HsOrc4-dependent DNA remodeling of the ori-β DHFR replicator
  3. Is Iron Chelation Important in Preventing Glycation of Bovine Serum Albumin in Vitro?
  4. The transition of the 37-kDa laminin receptor (RPSA) to higher molecular weight species: SUMOylation or artifact?
  5. Mechanical strain affects some microRNA profiles in pre-oeteoblasts.
  6. Sphingosine-1-phosphate induces the migration and angiogenesis of EPCs through the Akt signaling pathway via sphingosine-1-phosphate receptor 3/platelet-derived growth factor receptor-β
  7. Bioinformatics-based molecular classification of Arthrobacter plasmids
  8. Ras Transformation Overrides a Proliferation Defect Induced by Tpm3.1 Knockout
  9. The advanced lipoxidation end product precursor malondialdehyde induces IL-17E expression and skews lymphocytes to the Th17 subset
  10. On Application of Langevin Dynamics in Logarithmic Potential to Model Ion Channel Gate Activity
  11. Superoxide Dismutase 2 Polymorphisms and Osteoporosis in Asian Indians: A Genetic Association Analysis
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