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Ultra-trace arsenic and mercury speciation and determination in blood samples by ionic liquid-based dispersive liquid–liquid microextraction combined with flow injection-hydride generation/cold vapor atomic absorption spectroscopy

  • Hamid Shirkhanloo , Aisan Khaligh , Hassan Zavvar Mousavi EMAIL logo , Mohammad Mehdi Eskandari and Ali Akbar Miran-Beigi
Published/Copyright: March 19, 2015
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Chemical Papers
From the journal Chemical Papers Volume 69 Issue 6

Abstract

A simple, fast, and sensitive method for speciation and determination of As (III, V) and Hg (II, R) in human blood samples based on ionic liquid-dispersive liquid-liquid microextraction (IL-DLLME) and flow injection hydride generation/cold vapor atomic absorption spectrometry (FI-HG/CV-AAS) has been developed. Tetraethylthiuram disulfide, mixed ionic liquids (hydrophobic and hydrophilic ILs) and acetone were used in the DLLME step as the chelating agent, extraction and dispersive solvents, respectively. Using a microwave assisted-UV system, organic mercury (R-Hg) was converted to Hg(II) and total mercury amount was measured in blood samples by the presented method. Total arsenic content was determined by reducing As(V) to As(III) with potassium iodide and ascorbic acid in a hydrochloric acid solution. Finally, As(V) and R-Hg were determined by mathematically subtracting the As(III) and Hg(II) content from the total arsenic and mercury, respectively. Under optimum conditions, linear range and detection limit (3σ) of 0.1-5.0 μg L−1 and 0.02 μg L−1 for As(III) and 0.15-8.50 μg L−1 and 0.03 μg L−1 for Hg(II) were achieved, respectively, at low RSD values of < 4 % (N = 10). The developed method was successfully applied to determine the ultratrace amounts of arsenic and mercury species in blood samples; the validation of the method was performed using standard reference materials.

Keywords: arsenic; mercury; microwave assisted-UV system; ionic liquid-based dispersive liquid- liquid microextraction; hydride generation/cold vapor atomic absorption spectrometry

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Received: 2014-8-23
Revised: 2014-11-1
Accepted: 2014-11-6
Published Online: 2015-3-19
Published in Print: 2015-6-1

© 2015 Institute of Chemistry, Slovak Academy of Sciences

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https://doi.org/10.1515/chempap-2015-0086
Keywords for this article
arsenic; mercury; microwave assisted-UV system; ionic liquid-based dispersive liquid- liquid microextraction; hydride generation/cold vapor atomic absorption spectrometry

Articles in the same Issue

  1. Laser microsampling and multivariate methods in provenance studies of obsidian artefacts
  2. Ultra-trace arsenic and mercury speciation and determination in blood samples by ionic liquid-based dispersive liquid–liquid microextraction combined with flow injection-hydride generation/cold vapor atomic absorption spectroscopy
  3. Determination of formaldehyde by flow injection analysis with spectrophotometric detection exploiting brilliant green–sulphite reaction
  4. Passive sampling application to control air quality in interior of new vehicles
  5. Low-temperature enzymatic hydrolysis resolution in mini-emulsion media
  6. Performance and characterisation of CeO2–TiO2–WO3 catalysts for selective catalytic reduction of NO with NH3
  7. Catalytic wet peroxide oxidation of m-cresol over Fe/γ-Al2O3 and Fe–Ce/γ-Al2O3
  8. Morphology, structure, and photoactivity of two types of graphene oxide–TiO2 composites
  9. A novel efficient magnetic core–zeolitic shell nanocatalyst system: preparation, characterization and activity
  10. Characterisation and coagulation performance of polysilicate–ferric–zinc
  11. Antioxidant activity of rosemary extracts in solution and embedded in polymeric systems
  12. Comparison of selected aroma compounds in cultivars of sea buckthorn (Hippophae rhamnoides L.)
  13. Thio-click approach to the synthesis of stable glycomimetics
  14. Synthesis of ether-linked [60]fullerene glycoconjugates by nucleophilic cyclopropanation
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