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1,2-Dioleoylglycerol method for pancreatic lipase catalytic activity in serum
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Yoshiaki Iizuka
Published/Copyright:
November 30, 2011
Abstract
Background: There is a need for a pancreatic lipase (LIP) reference assay to provide an accurate base to which routine methods can be traceable.
Methods: This study developed a novel LIP assay method in which 1,2-dioleoylglycerol (DODG) is the substrate and LIP activity is measured in a coupled enzymatic reaction from the increase in absorbance at 340 nm with production of NADPH.
Results: With this method, LIP activity was linear up to 440 U/L (8-times expected upper limit of physiological concentration). When assayed manually, the between-laboratory variation for six samples surveyed at five laboratories was 3.80–26.4% (CV) for samples containing about 20–290 U/L LIP activity; when assayed using an automated analyzer, the range was 1.86–4.86% (four laboratories). Interference by >5 mmol/L glycerol and low specificity with post-heparin samples were noted, but in practice these are avoidable. Precision analyzed by automated assay of 49 samples twice in random order produced a covariance of 2.27 U/L, which is comparable to routine methods, and good correlations were obtained with five routine methods.
Conclusions: Although further studies are required, the DODG method may be likely applicable as one candidate reference method.
Received: 2011-8-11
Accepted: 2011-11-11
Published Online: 2011-11-30
Published in Print: 2012-03-01
©2012 by Walter de Gruyter Berlin Boston
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Keywords for this article
1,2-dioleoylglycerol;
lipase;
pancreas;
pancreatic lipase;
reference method
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