Article
Licensed
Unlicensed
Requires Authentication
Trueness in the measurement of haemoglobin: consensus or reference method?
-
Kristel J.M. Boonen
,
Joyce Curvers
Published/Copyright:
November 10, 2011
Abstract
Background: For the measurement of haemoglobin a reference method exists: the haemiglobincyanide method. However, a Dutch external quality assessment organization does not use this method in the evaluation of trueness of results. The aim of this work was to assess whether trueness was compromised by the use of a consensus value.
Methods: Five Cell Dyn Sapphires (Abbott) in three independent locations were used to measure haemoglobin concentration. Results were compared to the reference method (haemiglobincyanide). Patient samples with a distribution over clinically relevant concentrations (Hb 2.5–10.2 mmol/L) were used next to samples from external quality assessment rounds. Passing and Bablok regression analysis and Bland-Altman plots were used to evaluate any systematic deviation.
Results: Results measured on the Cell Dyn Sapphires deviated significantly from the results obtained with the reference method. Remarkably, consensus results from external quality control samples also deviated significantly from the reference method.
Conclusions: A significant negative bias exists in the measurement of haemoglobin on Cell Dyn Sapphires. Additionally, the consensus value as reported in external quality control assessment also shows an even greater significant negative bias compared to the reference method. As a reference method is available, external quality assessment would benefit from using this method instead of a consensus value to evaluate trueness.
Received: 2011-10-7
Accepted: 2011-10-18
Published Online: 2011-11-10
Published in Print: 2012-03-01
©2012 by Walter de Gruyter Berlin Boston
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- Editorials
- An appeal to medical journal editors: the need for a full description of laboratory methods and specimen handling in clinical study reports
- Cancer diagnosis: from dogs to DNA or from DNA to dogs?
- The never-ending search of an acceptable compromise for pancreatic lipase standardisation
- Review
- Hereditary breast cancer: beyond BRCA genetic analysis; PALB2 emerges
- Mini Review
- Canine olfactory detection of cancer versus laboratory testing: myth or opportunity?
- Genetics and Molecular Diagnostics
- SLCO1B1 gene variability influences lipid-lowering efficacy on simvastatin therapy in Southern Brazilians
- Elevated plasma matrix metalloproteinase-9 protein and its gene polymorphism in patients with community-acquired pneumonia
- General Clinical Chemistry and Laboratory Medicine
- Minimum analytical quality specifications of inter-laboratory comparisons: agreement among Spanish EQAP organizers
- Monitoring quality indicators in laboratory medicine does not automatically result in quality improvement
- Speed of sample transportation by a pneumatic tube system can influence the degree of hemolysis
- 1,2-Dioleoylglycerol method for pancreatic lipase catalytic activity in serum
- Serum lactate dehydrogenase levels significantly correlate with radiological extent of disease and spirometric values in patients with silicosis due to denim sandblasting
- Clinical comparison of new monoclonal antibody-based nephelometric assays for free light chain kappa and lambda to polyclonal antibody-based assays and immunofixation electrophoresis
- The relationship between antinuclear antibody data and antibodies against extractable nuclear antigens in a large laboratory cohort
- Anti-neutrophil cytoplasmic antibodies (ANCA) specific for one or several antigens: useful markers for subtypes of ulcerative colitis and associated primary sclerosing cholangitis
- Trueness in the measurement of haemoglobin: consensus or reference method?
- Soluble transcobalamin receptor, sCD320, is present in human serum and relates to serum cobalamin – establishment and validation of an ELISA
- An on-line solid phase extraction procedure for the routine quantification of caspofungin by liquid chromatography-tandem mass spectrometry
- Effects of subcutaneous low molecular weight heparin and intravenous unfractionated heparin on serum S100 concentrations in patients with cerebrovascular diseases
- Urinary 8-hydroxy-2′-deoxyguanosine level and plasma paraoxonase 1 activity with Alzheimer’s disease
- A HPLC-MS method to detect and quantify guanfacine in urine
- Collection and storage requirements for urinary kidney injury molecule-1 (KIM-1) measurements in humans
- Kidney stones in a Mediterranean population from the south of Spain
- Reference Values and Biological Variations
- First trimester biochemistry at different maternal ages
- Age- and gender-related alteration in plasma advanced oxidation protein products (AOPP) and glycosaminoglycan (GAG) concentrations in physiological ageing
- Cancer Diagnostics
- Direct sequencing in cytological specimens as a useful strategy for detecting EGFR mutations in non-small cell lung cancer patients
- Investigation of lung cancer biomarkers by hyphenated separation techniques and chemometrics
- Letters to the Editor
- Aberrant results observed with four immuno-assays for total and free prostate-specific antigen (PSA) determination: a case-report
- Assay-dependent abnormalities in measurements of prostate-specific antigen in serum: an occasional occurrence, but of clinical significance
- Clinical comparison of new monoclonal antibody-based nephelometric assays for free light chain κ and λ to polyclonal antibody-based assays and immunofixation electrophoresis
- Agarose-gel electrophoresis for the diagnosis of mucopolysaccharidoses
- Leptin levels in exhaled breath condensate from asthmatic children: a pilot study
- Masthead
- Masthead
Keywords for this article
consensus;
external quality assessment;
haemiglobincyanide;
reference method
Articles in the same Issue
- Editorials
- An appeal to medical journal editors: the need for a full description of laboratory methods and specimen handling in clinical study reports
- Cancer diagnosis: from dogs to DNA or from DNA to dogs?
- The never-ending search of an acceptable compromise for pancreatic lipase standardisation
- Review
- Hereditary breast cancer: beyond BRCA genetic analysis; PALB2 emerges
- Mini Review
- Canine olfactory detection of cancer versus laboratory testing: myth or opportunity?
- Genetics and Molecular Diagnostics
- SLCO1B1 gene variability influences lipid-lowering efficacy on simvastatin therapy in Southern Brazilians
- Elevated plasma matrix metalloproteinase-9 protein and its gene polymorphism in patients with community-acquired pneumonia
- General Clinical Chemistry and Laboratory Medicine
- Minimum analytical quality specifications of inter-laboratory comparisons: agreement among Spanish EQAP organizers
- Monitoring quality indicators in laboratory medicine does not automatically result in quality improvement
- Speed of sample transportation by a pneumatic tube system can influence the degree of hemolysis
- 1,2-Dioleoylglycerol method for pancreatic lipase catalytic activity in serum
- Serum lactate dehydrogenase levels significantly correlate with radiological extent of disease and spirometric values in patients with silicosis due to denim sandblasting
- Clinical comparison of new monoclonal antibody-based nephelometric assays for free light chain kappa and lambda to polyclonal antibody-based assays and immunofixation electrophoresis
- The relationship between antinuclear antibody data and antibodies against extractable nuclear antigens in a large laboratory cohort
- Anti-neutrophil cytoplasmic antibodies (ANCA) specific for one or several antigens: useful markers for subtypes of ulcerative colitis and associated primary sclerosing cholangitis
- Trueness in the measurement of haemoglobin: consensus or reference method?
- Soluble transcobalamin receptor, sCD320, is present in human serum and relates to serum cobalamin – establishment and validation of an ELISA
- An on-line solid phase extraction procedure for the routine quantification of caspofungin by liquid chromatography-tandem mass spectrometry
- Effects of subcutaneous low molecular weight heparin and intravenous unfractionated heparin on serum S100 concentrations in patients with cerebrovascular diseases
- Urinary 8-hydroxy-2′-deoxyguanosine level and plasma paraoxonase 1 activity with Alzheimer’s disease
- A HPLC-MS method to detect and quantify guanfacine in urine
- Collection and storage requirements for urinary kidney injury molecule-1 (KIM-1) measurements in humans
- Kidney stones in a Mediterranean population from the south of Spain
- Reference Values and Biological Variations
- First trimester biochemistry at different maternal ages
- Age- and gender-related alteration in plasma advanced oxidation protein products (AOPP) and glycosaminoglycan (GAG) concentrations in physiological ageing
- Cancer Diagnostics
- Direct sequencing in cytological specimens as a useful strategy for detecting EGFR mutations in non-small cell lung cancer patients
- Investigation of lung cancer biomarkers by hyphenated separation techniques and chemometrics
- Letters to the Editor
- Aberrant results observed with four immuno-assays for total and free prostate-specific antigen (PSA) determination: a case-report
- Assay-dependent abnormalities in measurements of prostate-specific antigen in serum: an occasional occurrence, but of clinical significance
- Clinical comparison of new monoclonal antibody-based nephelometric assays for free light chain κ and λ to polyclonal antibody-based assays and immunofixation electrophoresis
- Agarose-gel electrophoresis for the diagnosis of mucopolysaccharidoses
- Leptin levels in exhaled breath condensate from asthmatic children: a pilot study
- Masthead
- Masthead
