Article
Licensed
Unlicensed Requires Authentication

Urinary 8-hydroxy-2′-deoxyguanosine level and plasma paraoxonase 1 activity with Alzheimer’s disease

  • , , , , and EMAIL logo
Published/Copyright: November 18, 2011
Clinical Chemistry and Laboratory Medicine
From the journal Clinical Chemistry and Laboratory Medicine Volume 50 Issue 3

Abstract

Background: Alzheimer’s disease (AD) is the most frequent cause of dementia and age is the most important risk factor for AD. Aging is associated with increased free radical production and oxidative stress plays an important role in the pathogenesis of AD. 8-Hydroxy-2′-deoxyguanosine (8-OHdG) is a biomarker indicating oxidative DNA damage. Paraoxonase 1 (PON1) is a high-density lipoprotein (HDL)-associated antioxidant enzyme and prevents especially oxidation of low-density lipoproteins. The aim of this study is to measure urinary 8-OHdG levels and serum PON1 activity in patients with AD.

Methods: A total of 21 elderly patients diagnosed with moderate AD (10 men and 11 women, aged 76±7.8 years) were included in the study. A total of 20 healthy elderly volunteers (11 men and nine women, aged 81±7.2 years) were enrolled as a control group. Levels of urinary 8-OHdG, serum PON1 activity and lipid profile were determined in patients and controls.

Results: Urinary 8-OHdG levels were significantly increased, but serum PON1 activity was significantly decreased in patients compared to controls. Lipid profile did not show a difference between the groups. There was a negative correlation between 8-OHdG levels and PON1 activity only in the patient group (r=–0.536). Analytical performance characteristics of the methods used were satisfactory.

Conclusions: In this study, evidence of increased oxidative DNA damage was determined in AD patients as well as decreased serum PON1 activity. Oxidant stress and oxidative DNA damage are important pathological processes in AD. The biomarkers, urinary 8-OHdG level and serum PON1 activity can be used to determine and monitor the status of patients with AD.

Keywords: Alzheimer’s disease; DNA damage; 8-hydroxy-2′-deoxyguanosine (8-OhdG); oxidative stress; paraoxonase
Corresponding author: Dogan Yucel, Department of Medical Biochemistry, Ankara Training and Research Hospital, Ministry of Health Ulucanlar Caddesi, Cebeci, Ankara 06340, Turkey Phone: +90-533 354 2006, Fax: +90-312 595 3212
Received: 2011-8-17
Accepted: 2011-10-31
Published Online: 2011-11-18
Published in Print: 2012-03-01

©2012 by Walter de Gruyter Berlin Boston

Articles in the same Issue

  1. Editorials
  2. An appeal to medical journal editors: the need for a full description of laboratory methods and specimen handling in clinical study reports
  3. Cancer diagnosis: from dogs to DNA or from DNA to dogs?
  4. The never-ending search of an acceptable compromise for pancreatic lipase standardisation
  5. Review
  6. Hereditary breast cancer: beyond BRCA genetic analysis; PALB2 emerges
  7. Mini Review
  8. Canine olfactory detection of cancer versus laboratory testing: myth or opportunity?
  9. Genetics and Molecular Diagnostics
  10. SLCO1B1 gene variability influences lipid-lowering efficacy on simvastatin therapy in Southern Brazilians
  11. Elevated plasma matrix metalloproteinase-9 protein and its gene polymorphism in patients with community-acquired pneumonia
  12. General Clinical Chemistry and Laboratory Medicine
  13. Minimum analytical quality specifications of inter-laboratory comparisons: agreement among Spanish EQAP organizers
  14. Monitoring quality indicators in laboratory medicine does not automatically result in quality improvement
  15. Speed of sample transportation by a pneumatic tube system can influence the degree of hemolysis
  16. 1,2-Dioleoylglycerol method for pancreatic lipase catalytic activity in serum
  17. Serum lactate dehydrogenase levels significantly correlate with radiological extent of disease and spirometric values in patients with silicosis due to denim sandblasting
  18. Clinical comparison of new monoclonal antibody-based nephelometric assays for free light chain kappa and lambda to polyclonal antibody-based assays and immunofixation electrophoresis
  19. The relationship between antinuclear antibody data and antibodies against extractable nuclear antigens in a large laboratory cohort
  20. Anti-neutrophil cytoplasmic antibodies (ANCA) specific for one or several antigens: useful markers for subtypes of ulcerative colitis and associated primary sclerosing cholangitis
  21. Trueness in the measurement of haemoglobin: consensus or reference method?
  22. Soluble transcobalamin receptor, sCD320, is present in human serum and relates to serum cobalamin – establishment and validation of an ELISA
  23. An on-line solid phase extraction procedure for the routine quantification of caspofungin by liquid chromatography-tandem mass spectrometry
  24. Effects of subcutaneous low molecular weight heparin and intravenous unfractionated heparin on serum S100 concentrations in patients with cerebrovascular diseases
  25. Urinary 8-hydroxy-2′-deoxyguanosine level and plasma paraoxonase 1 activity with Alzheimer’s disease
  26. A HPLC-MS method to detect and quantify guanfacine in urine
  27. Collection and storage requirements for urinary kidney injury molecule-1 (KIM-1) measurements in humans
  28. Kidney stones in a Mediterranean population from the south of Spain
  29. Reference Values and Biological Variations
  30. First trimester biochemistry at different maternal ages
  31. Age- and gender-related alteration in plasma advanced oxidation protein products (AOPP) and glycosaminoglycan (GAG) concentrations in physiological ageing
  32. Cancer Diagnostics
  33. Direct sequencing in cytological specimens as a useful strategy for detecting EGFR mutations in non-small cell lung cancer patients
  34. Investigation of lung cancer biomarkers by hyphenated separation techniques and chemometrics
  35. Letters to the Editor
  36. Aberrant results observed with four immuno-assays for total and free prostate-specific antigen (PSA) determination: a case-report
  37. Assay-dependent abnormalities in measurements of prostate-specific antigen in serum: an occasional occurrence, but of clinical significance
  38. Clinical comparison of new monoclonal antibody-based nephelometric assays for free light chain κ and λ to polyclonal antibody-based assays and immunofixation electrophoresis
  39. Agarose-gel electrophoresis for the diagnosis of mucopolysaccharidoses
  40. Leptin levels in exhaled breath condensate from asthmatic children: a pilot study
  41. Masthead
  42. Masthead
https://doi.org/10.1515/cclm.2011.792
Keywords for this article
Alzheimer’s disease; DNA damage; 8-hydroxy-2′-deoxyguanosine (8-OhdG); oxidative stress; paraoxonase

Articles in the same Issue

  1. Editorials
  2. An appeal to medical journal editors: the need for a full description of laboratory methods and specimen handling in clinical study reports
  3. Cancer diagnosis: from dogs to DNA or from DNA to dogs?
  4. The never-ending search of an acceptable compromise for pancreatic lipase standardisation
  5. Review
  6. Hereditary breast cancer: beyond BRCA genetic analysis; PALB2 emerges
  7. Mini Review
  8. Canine olfactory detection of cancer versus laboratory testing: myth or opportunity?
  9. Genetics and Molecular Diagnostics
  10. SLCO1B1 gene variability influences lipid-lowering efficacy on simvastatin therapy in Southern Brazilians
  11. Elevated plasma matrix metalloproteinase-9 protein and its gene polymorphism in patients with community-acquired pneumonia
  12. General Clinical Chemistry and Laboratory Medicine
  13. Minimum analytical quality specifications of inter-laboratory comparisons: agreement among Spanish EQAP organizers
  14. Monitoring quality indicators in laboratory medicine does not automatically result in quality improvement
  15. Speed of sample transportation by a pneumatic tube system can influence the degree of hemolysis
  16. 1,2-Dioleoylglycerol method for pancreatic lipase catalytic activity in serum
  17. Serum lactate dehydrogenase levels significantly correlate with radiological extent of disease and spirometric values in patients with silicosis due to denim sandblasting
  18. Clinical comparison of new monoclonal antibody-based nephelometric assays for free light chain kappa and lambda to polyclonal antibody-based assays and immunofixation electrophoresis
  19. The relationship between antinuclear antibody data and antibodies against extractable nuclear antigens in a large laboratory cohort
  20. Anti-neutrophil cytoplasmic antibodies (ANCA) specific for one or several antigens: useful markers for subtypes of ulcerative colitis and associated primary sclerosing cholangitis
  21. Trueness in the measurement of haemoglobin: consensus or reference method?
  22. Soluble transcobalamin receptor, sCD320, is present in human serum and relates to serum cobalamin – establishment and validation of an ELISA
  23. An on-line solid phase extraction procedure for the routine quantification of caspofungin by liquid chromatography-tandem mass spectrometry
  24. Effects of subcutaneous low molecular weight heparin and intravenous unfractionated heparin on serum S100 concentrations in patients with cerebrovascular diseases
  25. Urinary 8-hydroxy-2′-deoxyguanosine level and plasma paraoxonase 1 activity with Alzheimer’s disease
  26. A HPLC-MS method to detect and quantify guanfacine in urine
  27. Collection and storage requirements for urinary kidney injury molecule-1 (KIM-1) measurements in humans
  28. Kidney stones in a Mediterranean population from the south of Spain
  29. Reference Values and Biological Variations
  30. First trimester biochemistry at different maternal ages
  31. Age- and gender-related alteration in plasma advanced oxidation protein products (AOPP) and glycosaminoglycan (GAG) concentrations in physiological ageing
  32. Cancer Diagnostics
  33. Direct sequencing in cytological specimens as a useful strategy for detecting EGFR mutations in non-small cell lung cancer patients
  34. Investigation of lung cancer biomarkers by hyphenated separation techniques and chemometrics
  35. Letters to the Editor
  36. Aberrant results observed with four immuno-assays for total and free prostate-specific antigen (PSA) determination: a case-report
  37. Assay-dependent abnormalities in measurements of prostate-specific antigen in serum: an occasional occurrence, but of clinical significance
  38. Clinical comparison of new monoclonal antibody-based nephelometric assays for free light chain κ and λ to polyclonal antibody-based assays and immunofixation electrophoresis
  39. Agarose-gel electrophoresis for the diagnosis of mucopolysaccharidoses
  40. Leptin levels in exhaled breath condensate from asthmatic children: a pilot study
  41. Masthead
  42. Masthead
Downloaded on 12.4.2026 from https://www.degruyterbrill.com/document/doi/10.1515/cclm.2011.792/html
Scroll to top button