Impact of blood cell counts and volumes on glucose concentration in uncentrifuged serum and lithium-heparin blood tubes
-
Giuseppe Lippi
,
Gian Luca Salvagno
Abstract
Background:
Although it is known that glucose concentration exhibits a time-dependent decay in uncentrifuged serum and lithium-heparin blood tubes, no evidence exists on how this variation may depend on blood cell counts (CBC) and volumes.
Methods:
Venous blood was drawn from 30 non fasting healthy volunteers into three serum and three lithium-heparin tubes. One serum and lithium-heparin tubes were centrifuged within 15 min after collection and glucose was measured with a hexokinase assay. The second and third serum and lithium-heparin tubes were maintained at room temperature for 1 and 2 h after the first tubes were centrifuged. These other tubes were then centrifuged and glucose was measured. CBC was performed in the first lithium-heparin tube, before centrifugation.
Results:
The mean decrease of glucose was higher in lithium-heparin plasma than in serum (0.33 vs. 0.24 mmol/L/h; p<0.001). Glucose concentration decreased by 7% and 5% per hour in lithium-heparin plasma and serum, respectively. In univariate analysis, the absolute decrease of glucose concentration was associated with sex (higher in men than in women), red blood cell (RBC) count, hematocrit, white blood cell (WBC) count, neutrophils and monocytes in both lithium-heparin plasma and serum. In multivariate analysis, the decrease of glucose concentration remained independently associated with RBC, WBC, neutrophils and monocytes in both sample matrices. No significant association was found with platelet number and erythrocyte or platelet volume.
Conclusions:
Glucose concentration decrease in uncentrifuged lithium-heparin and serum tubes depends on the baseline number of RBC, WBC, neutrophils and monocytes within the tubes.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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©2018 Walter de Gruyter GmbH, Berlin/Boston
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Articles in the same Issue
- Frontmatter
- Editorial
- Observing an analyzer’s operational life cycle: a useful management tool for clinical laboratories
- Reviews
- Personalized laboratory medicine: a patient-centered future approach
- Circular RNAs: a new class of biomarkers as a rising interest in laboratory medicine
- Mini Review
- Impact of interactions between drugs and laboratory test results on diagnostic test interpretation – a systematic review
- Opinion Paper
- Uncertainty in measurement and total error: different roads to the same quality destination?
- Guidelines and Recommendations
- Joint EFLM-COLABIOCLI Recommendation for venous blood sampling
- General Clinical Chemistry and Laboratory Medicine
- Evidence for the positive impact of ISO 9001 and ISO 15189 quality systems on laboratory performance – evaluation of immunohaematology external quality assessment results during 19 years in Austria
- Effects of high-dose, intravenous lipid emulsion on laboratory tests in humans: a randomized, placebo-controlled, double-blind, clinical crossover trial
- Commutability of the certified reference materials for the standardization of β-amyloid 1-42 assay in human cerebrospinal fluid: lessons for tau and β-amyloid 1-40 measurements
- Failure rate prediction of equipment: can Weibull distribution be applied to automated hematology analyzers?
- Evaluation of serum alkaline phosphatase measurement through the 4-year trueness verification program in China
- Increased serum concentrations of soluble ST2 predict mortality after burn injury
- The clinical significance of borderline results of the Elia CTD Screen assay
- Reference Values and Biological Variations
- Reference intervals for 33 biochemical analytes in healthy Indian population: C-RIDL IFCC initiative
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- Cardiovascular Diseases
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- Diabetes
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- Detection of Plasmodium falciparum using automated digital cell morphology analyzer Sysmex DI-60
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- Wet absorptive microsampling at home for HbA1c monitoring in diabetic children
- Serum endocan levels in patients with chronic obstructive pulmonary disease: a potential role in the evaluation of susceptibility to exacerbation
- Analytical and clinical validation of the new Roche Elecsys Vitamin D Total II assay
- Analytical validation of two second generation thyroglobulin immunoassays (Roche and Thermo Fisher)
- Omission of preservatives during 24-h of urine collection for the analysis of fractionated metanephrines enhance patient convenience
- Transient monoclonal gammopathy in a 2-year-old child with combined viral and bacterial infection
- Nephelometric assay of urine free light chains: an alternative and early clinical test for Bence-Jones protein quantification
- Congress Abstracts
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- 50th National Congress of the Italian Society of Clinical Biochemistry and Clinical Molecular Biology (SIBioC – Laboratory Medicine)
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