Binding of bromocresol green and bromocresol purple to albumin in hemodialysis patients
-
Sigurd Delanghe
und
Marijn M. Speeckaert
Abstract
Background:
Colorimetric albumin assays based on binding to bromocresol purple (BCP) and bromocresol green (BCG) yield different results in chronic kidney disease. Altered dye binding of carbamylated albumin has been suggested as a cause. In the present study, a detailed analysis was carried out in which uremic toxins, acute phase proteins and Kt/V, a parameter describing hemodialysis efficiency, were compared with colorimetrically assayed (BCP and BCG) serum albumin.
Methods:
Albumin was assayed using immunonephelometry on a BN II nephelometer and colorimetrically based on, respectively, BCP and BCG on a Modular P analyzer. Uremic toxins were assessed using high-performance liquid chromatography. Acute phase proteins (C-reactive protein and α1-acid glycoprotein) and plasma protein α2-macroglobulin were assayed nephelometrically. In parallel, Kt/V was calculated.
Results:
Sixty-two serum specimens originating from hemodialysis patients were analyzed. Among the uremic toxins investigated, total para-cresyl sulfate (PCS) showed a significant positive correlation with the BCP/BCG ratio. The serum α1-acid glycoprotein concentration correlated negatively with the BCP/BCG ratio. The BCP/BCG ratio showed also a negative correlation with Kt/V.
Conclusions:
In renal insufficiency, the BCP/BCG ratio of serum albumin is affected by multiple factors: next to carbamylation, uremic toxins (total PCS) and α1-acid glycoprotein also play a role.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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©2018 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Editorial
- Analytical quality: an unfinished journey
- Reviews
- Update in diagnosis and management of primary aldosteronism
- Diagnosis biomarkers in acute intestinal ischemic injury: so close, yet so far
- Opinion Papers
- Irregular analytical errors in diagnostic testing – a novel concept
- A Black Swan in clinical laboratory practice: the analytical error due to interferences in immunoassay methods
- General Clinical Chemistry and Laboratory Medicine
- Reaching consensus on communication of critical laboratory results using a collective intelligence method
- Stability of routine biochemical analytes in whole blood and plasma/serum: focus on potassium stability from lithium heparin
- GFR estimation based on standardized creatinine and cystatin C: a European multicenter analysis in older adults
- Binding of bromocresol green and bromocresol purple to albumin in hemodialysis patients
- Interlaboratory variability of urinary iodine measurements
- The venous thromboembolic risk and the clot wave analysis: a useful relationship?
- Hematology and Coagulation
- Autovalidation and automation of the postanalytical phase of routine hematology and coagulation analyses in a university hospital laboratory
- Reference Values and Biological Variations
- Indirect method for validating transference of reference intervals
- Differences in levels of albumin, ALT, AST, γ-GT and creatinine in frail, moderately healthy and healthy elderly individuals
- Cancer Diagnostics
- Serum exosomal hnRNPH1 mRNA as a novel marker for hepatocellular carcinoma
- Intragenic hypomethylation of DNMT3A in patients with myelodysplastic syndrome
- Cardiovascular Diseases
- Evaluation of analytical performance of a new high-sensitivity immunoassay for cardiac troponin I
- MEF2C loss-of-function mutation associated with familial dilated cardiomyopathy
- Letter to the Editor
- Hyperuricemia does not seem to be an independent risk factor for coronary heart disease
- Reply to: Hyperuricemia does not seem to be an independent risk factor for coronary heart disease
- Preanalytics of ammonia: stability, transport and temperature of centrifugation
- Influence of delayed separation of plasma from whole blood on Cu, I, Mn, Se, and Zn plasma concentrations
- Copeptin as a diagnostic and prognostic biomarker in patients admitted to Emergency Department with syncope, presyncope and vertiginous syndrome
- Development of an internally controlled quantitative PCR to measure total cell-associated HIV-1 DNA in blood
- Selective changes in cholesterol metabolite levels in plasma of breast cancer patients after tumor removal
- Athletes beware before throwing towels to audiences
Artikel in diesem Heft
- Frontmatter
- Editorial
- Analytical quality: an unfinished journey
- Reviews
- Update in diagnosis and management of primary aldosteronism
- Diagnosis biomarkers in acute intestinal ischemic injury: so close, yet so far
- Opinion Papers
- Irregular analytical errors in diagnostic testing – a novel concept
- A Black Swan in clinical laboratory practice: the analytical error due to interferences in immunoassay methods
- General Clinical Chemistry and Laboratory Medicine
- Reaching consensus on communication of critical laboratory results using a collective intelligence method
- Stability of routine biochemical analytes in whole blood and plasma/serum: focus on potassium stability from lithium heparin
- GFR estimation based on standardized creatinine and cystatin C: a European multicenter analysis in older adults
- Binding of bromocresol green and bromocresol purple to albumin in hemodialysis patients
- Interlaboratory variability of urinary iodine measurements
- The venous thromboembolic risk and the clot wave analysis: a useful relationship?
- Hematology and Coagulation
- Autovalidation and automation of the postanalytical phase of routine hematology and coagulation analyses in a university hospital laboratory
- Reference Values and Biological Variations
- Indirect method for validating transference of reference intervals
- Differences in levels of albumin, ALT, AST, γ-GT and creatinine in frail, moderately healthy and healthy elderly individuals
- Cancer Diagnostics
- Serum exosomal hnRNPH1 mRNA as a novel marker for hepatocellular carcinoma
- Intragenic hypomethylation of DNMT3A in patients with myelodysplastic syndrome
- Cardiovascular Diseases
- Evaluation of analytical performance of a new high-sensitivity immunoassay for cardiac troponin I
- MEF2C loss-of-function mutation associated with familial dilated cardiomyopathy
- Letter to the Editor
- Hyperuricemia does not seem to be an independent risk factor for coronary heart disease
- Reply to: Hyperuricemia does not seem to be an independent risk factor for coronary heart disease
- Preanalytics of ammonia: stability, transport and temperature of centrifugation
- Influence of delayed separation of plasma from whole blood on Cu, I, Mn, Se, and Zn plasma concentrations
- Copeptin as a diagnostic and prognostic biomarker in patients admitted to Emergency Department with syncope, presyncope and vertiginous syndrome
- Development of an internally controlled quantitative PCR to measure total cell-associated HIV-1 DNA in blood
- Selective changes in cholesterol metabolite levels in plasma of breast cancer patients after tumor removal
- Athletes beware before throwing towels to audiences
