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Evaluation of Mindray BC-6800 body fluid mode for automated cerebrospinal fluid cell counting

  • Sabrina Buoro EMAIL logo , Michela Seghezzi , Tommaso Mecca , Mauro Vavassori , Alberto Crippa and Antonio La Gioia
Published/Copyright: April 21, 2016
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Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 54 Issue 11

Abstract

Background:

Cellular analysis in cerebrospinal fluid (CSF) provides important diagnostic information in various medical conditions. The aim of this study was to evaluate the application of Mindray BC-6800 body fluid (BF) mode in cytometric analysis of CSF compared to light microscopy (LM).

Methods:

One hundred and twenty-nine consecutive CSF samples were analyzed by BC-6800-BF mode as well as by LM. The study also included limits of blank (LoB), limit of detection (LoD), limit of quantitation (LoQ), carryover and linearity.

Results

White blood cells LoQ was 4.0×106 cells/L. Linearity was good and carryover was negligible. As for the total and white blood cells, the BC-6800-BF parameters vs. LM showed both bias ranged from –10.28 to 0.06×106 cells/L. Polymorphonuclear and mononuclear cells ranged from 6.64 to 10.90%. For white blood cell the diagnostic agreement was 93% at the cut-off >5.0×106 cells/L, and for polymorphonuclear and mononuclear at the cut-off >50% was 91% and 92%, respectively.

Conclusions:

BC-6800-BF offers rapid and accurate counts in clinically relevant concentration ranges, replacing LM for most samples. However, in samples with abnormal cell counts or with abnormal white blood cell differential scattergrams the need to microscopic review for a correct clinical outcome remains.

Keywords: automated cell counting; cerebrospinal fluid; light microscopy; Mindray BC-6800

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2015-11-8
Accepted: 2016-3-4
Published Online: 2016-4-21
Published in Print: 2016-11-1

©2016 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/cclm-2015-1092
Keywords for this article
automated cell counting; cerebrospinal fluid; light microscopy; Mindray BC-6800

Articles in the same Issue

  1. Frontmatter
  2. Editorials
  3. CCLM Award for the Most Cited Paper Recently Published
  4. Laboratory economics. Risk or opportunity?
  5. Reviews
  6. Molecular diagnosis and precision medicine in allergy management
  7. The insulin autoimmune syndrome (IAS) as a cause of hypoglycaemia: an update on the pathophysiology, biochemical investigations and diagnosis
  8. Opinion Paper
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  10. Genetics and Molecular Diagnostics
  11. Time and tumor type (primary or metastatic) do not influence the detection of BRAF/NRAS mutations in formalin fixed paraffin embedded samples from melanomas
  12. False low holotranscobalamin levels in a patient with a novel TCN2 mutation
  13. General Clinical Chemistry and Laboratory Medicine
  14. Quality performance of laboratory testing in pharmacies: a collaborative evaluation
  15. Cost evaluation of clinical laboratory in Taiwan’s National Health System by using activity-based costing
  16. Impact of sample processing on the measurement of circulating microparticles: storage and centrifugation parameters
  17. HbA1c: EQA in Germany, Belgium and the Netherlands using fresh whole blood samples with target values assigned with the IFCC reference system
  18. Iohexol clearance in unstable critically ill patients: a tool to assess glomerular filtration rate
  19. Characteristics of Chinese patients with antiphospholipid syndrome and the ability of lupus anticoagulant assays to identify them
  20. Reference Values and Biological Variations
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  23. Evaluation of Mindray BC-6800 body fluid mode for automated cerebrospinal fluid cell counting
  24. Cancer Diagnostics
  25. Increased sialylation and reduced fucosylation of exfoliated cervical cells are potential markers of carcinogenesis in the cervix
  26. Cardiovascular Diseases
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  28. Infectious Diseases
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  32. Quality of reporting of diagnostic test accuracy studies in medical laboratory journals
  33. Impact of under-filled blood collection tubes containing K2EDTA and K3EDTA as anticoagulants on automated complete blood count (CBC) testing
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