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Identification of molecular species of oxidized triglyceride in plasma and its distribution in lipoproteins

  • Rojeet Shrestha , Shu-Ping Hui EMAIL logo , Yusuke Miura , Akiko Yagi , Yuji Takahashi , Seiji Takeda , Hirotoshi Fuda and Hitoshi Chiba
Published/Copyright: March 14, 2015
Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 53 Issue 11

Abstract

Background: The role of triglycerides carried in the triglyceride-rich lipoproteins (TRL) in the progression of atherosclerosis is uncertain. Identification of oxidized triglycerides and its possible association with atherosclerosis were largely ignored. Here we applied mass spectrometric approach to detect and identify triglyceride hydroperoxides (TGOOH) in human plasma and lipoproteins.

Methods: EDTA plasma was collected from healthy human volunteers (n=9) after 14–16 h of fasting. Very low-density lipoprotein (VLDL) (d<1.006) and intermediate-density lipoprotein (IDL) (d=1.006–1.019) were isolated from the plasma (n=6) by sequential ultracentrifugation in KBr, followed by the isolation of LDL and high-density lipoprotein (HDL) using size-exclusion high-performance liquid chromatography (HPLC). Total lipids from the plasma and isolated lipoproteins were extracted, and analyzed for the detection and identification of TGOOH using liquid chromatography/LTQ ion trap Orbitrap mass spectrometry. All the processes, from specimen collection to the mass spectrometric analysis, were carried out at 4 °C in the presence of antioxidant to prevent oxidation of lipoproteins.

Results: We identified 11 molecular species of TGOOH in either plasma or VLDL and IDL, of which TGOOH-18:1/18:2/16:0, TGOOH-18:1/18:1/16:0, TGOOH-16:0/18:2/16:0, TGOOH-18:1/18:1/18:1, and TGOOH-16:0/20:4/16:0 were most dominant. These TGOOH molecules are carried by TRL but not by LDL and HDL. Mean concentration of TGOOH in plasma, VLDL and IDL were, respectively, 56.1±25.6, 349.8±253.6 and 512.5±173.2 μmol/mol of triglycerides.

Conclusions: This is the first report to identify several molecular species of oxidized triglycerides in TRL. Presence of oxidized triglyceride may contribute to the atherogenicity of TRL. Further work is needed to elucidate the association of the oxidized triglyceride in atherosclerosis.

Keywords: intermediate-density lipoprotein; liquid chromatography/mass spectrometry; oxidized triglycerides; triglyceride-rich lipoproteins; very low-density lipoprotein
Corresponding author: Shu-Ping Hui, MD, PhD, Faculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Sapporo 060-0812, Japan, Phone/Fax: +81 11 7063693, E-mail:

Acknowledgments

This work was supported by the Regional Innovation Strategy Support Program, Sapporo Health Innovation “Smart-H”, of The Ministry of Education, Culture, Sports, Sciences and Technology, Japan and partly by a Grant-in-Aid for scientific Research from the Japan Society for promotion of Sciences, Japan. A part of this study was presented during annual meeting of American Association of Clinical Chemistry (AACC) and has been recognized as “National Academy of Clinical Biochemistry (NACB) distinguished Abstract Award 2013”.

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Financial support: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Supplemental Material

The online version of this article (DOI: 10.1515/cclm-2014-1088) offers supplementary material, available to authorized users.

Received: 2014-11-6
Accepted: 2015-2-11
Published Online: 2015-3-14
Published in Print: 2015-10-1

©2015 by De Gruyter

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https://doi.org/10.1515/cclm-2014-1088
Keywords for this article
intermediate-density lipoprotein; liquid chromatography/mass spectrometry; oxidized triglycerides; triglyceride-rich lipoproteins; very low-density lipoprotein

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  1. Frontmatter
  2. Editorials
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  4. The standardization of the urine albumin assays: no longer deferrable
  5. Reviews
  6. The role of telomeres and vitamin D in cellular aging and age-related diseases
  7. Atypical hemolytic uremic syndrome: from diagnosis to treatment
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  11. Genetics and Molecular Diagnostics
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  14. Uncertainty in measurement for 43 biochemistry, immunoassay, and hemostasis routine analytes evaluated by a method using only external quality assessment data
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  22. Whole blood thromboelastometry profiles in women with preeclampsia
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