Long-term biological variability of galectin-3 after heart transplantation
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Janka Franeková
Abstract
Background: Galectin-3 is an emerging biomarker of heart failure and of myocardial fibrosis risk. Monitoring of galectin-3 is essential during treatment with galectin-3 inhibitors. The aim of our study was to assess long-term biological variability in a specific group of unhealthy subjects.
Methods: The biological variability of galectin-3 was measured in a group of 44 patients after heart transplantation (HTx). Six samples were taken from each patient during a 12-month period. Galectin-3 was measured with an Abbott Architect automated immunoassay.
Results: Intraindividual (CVi) and interindividual (CVg) variabilities were calculated together with the reference change value (RCV), the log-normal RCV for increase (RCV+), and the log-normal RCV for decrease (RCV–). The CVi, CVg, RCV, RCV+, and RCV– were 28.2%, 35.6%, 78.6%, 116%, and –53.7%, respectively. The index of individuality was 0.79.
Conclusions: The concentrations of galectin-3 in patients followed 12 months after HTx fluctuated around the homeostatic point, with CVi of approximately 28%. RCVs of +116% (log-normal increase) and –54% (log-normal decrease) mean that the concentration of galectin-3 would need to approximately double or decrease by half to indicate a new process.
Acknowledgments
Support for this study was provided by Abbott Laboratories, which provided reagents.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Financial support: None declared.
Employment or leadership: None declared.
Honorarium: Antonín Jabor accepted speaker’s honoraria from Abbott Laboratories and Roche Diagnostics. For the other authors, no conflicts of interest are declared.
Competing interests: The funding organisation(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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©2015 by De Gruyter
Artikel in diesem Heft
- Frontmatter
- Editorial
- Harmonisation of specialist training and continuing professional development in laboratory medicine: a long but necessary journey
- Reviews
- Laboratory medicine in the European Union
- Role of leptin in female reproduction
- Mini Review
- Interrelatedness between C-reactive protein and oxidized low-density lipoprotein
- General Clinical Chemistry and Laboratory Medicine
- Comparison of approaches and measurement of continuing professional development for specialists in laboratory medicine within four European countries
- Point-of-care urine albumin in general practice offices: effect of participation in an external quality assurance scheme
- Triple positive antiphospholipid antibody profile in outpatients with tests for lupus anticoagulants
- Effects of cigarette smoking on circulating leukocytes and plasma cytokines in monozygotic twins
- Proteolysis is a confounding factor in the interpretation of faecal calprotectin
- Urinary neutrophil gelatinase-associated lipocalin and clinical outcomes in chronic kidney disease patients
- Cancer Diagnostics
- Quantification of adult T-cell leukemia/lymphoma cells using simple four-color flow cytometry
- Serum fucosylated haptoglobin in chronic liver diseases as a potential biomarker of hepatocellular carcinoma development
- Cardiovascular Diseases
- Increased serum homocitrulline concentrations are associated with the severity of coronary artery disease
- S100B concentrations increase perioperatively in jugular vein blood despite limited metabolic and inflammatory response to clinically uneventful carotid endarterectomy
- Long-term biological variability of galectin-3 after heart transplantation
- Diabetes
- Evaluation of a next generation direct whole blood enzymatic assay for hemoglobin A1c on the ARCHITECT c8000 chemistry system
- The 13C-glucose breath test is a valid non-invasive screening tool to identify metabolic syndrome in adolescents
- Comparison of a direct enzymatic assay and polyacrylamide tube gel electrophoresis for measurement of small, dense low-density lipoprotein cholesterol
- Letters to the Editor
- Comparison of Abbott Architect high-sensitivity troponin I in Rapid Serum Tubes and plasma
- Can EDTA, EDTA-fluoride, and buffered citrate tubes be used for measurement of HbA1c on the Bio-Rad D10?
- How to reduce EDTA contamination in laboratory specimens: a Tunisian experience
- Does mean platelet volume share any relationship with biochemical and hematological parameters?
- Association of retrospective markers of glycemia and the use of continuous glucose monitoring in white adults with type 2 diabetes mellitus – a preliminary report
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Artikel in diesem Heft
- Frontmatter
- Editorial
- Harmonisation of specialist training and continuing professional development in laboratory medicine: a long but necessary journey
- Reviews
- Laboratory medicine in the European Union
- Role of leptin in female reproduction
- Mini Review
- Interrelatedness between C-reactive protein and oxidized low-density lipoprotein
- General Clinical Chemistry and Laboratory Medicine
- Comparison of approaches and measurement of continuing professional development for specialists in laboratory medicine within four European countries
- Point-of-care urine albumin in general practice offices: effect of participation in an external quality assurance scheme
- Triple positive antiphospholipid antibody profile in outpatients with tests for lupus anticoagulants
- Effects of cigarette smoking on circulating leukocytes and plasma cytokines in monozygotic twins
- Proteolysis is a confounding factor in the interpretation of faecal calprotectin
- Urinary neutrophil gelatinase-associated lipocalin and clinical outcomes in chronic kidney disease patients
- Cancer Diagnostics
- Quantification of adult T-cell leukemia/lymphoma cells using simple four-color flow cytometry
- Serum fucosylated haptoglobin in chronic liver diseases as a potential biomarker of hepatocellular carcinoma development
- Cardiovascular Diseases
- Increased serum homocitrulline concentrations are associated with the severity of coronary artery disease
- S100B concentrations increase perioperatively in jugular vein blood despite limited metabolic and inflammatory response to clinically uneventful carotid endarterectomy
- Long-term biological variability of galectin-3 after heart transplantation
- Diabetes
- Evaluation of a next generation direct whole blood enzymatic assay for hemoglobin A1c on the ARCHITECT c8000 chemistry system
- The 13C-glucose breath test is a valid non-invasive screening tool to identify metabolic syndrome in adolescents
- Comparison of a direct enzymatic assay and polyacrylamide tube gel electrophoresis for measurement of small, dense low-density lipoprotein cholesterol
- Letters to the Editor
- Comparison of Abbott Architect high-sensitivity troponin I in Rapid Serum Tubes and plasma
- Can EDTA, EDTA-fluoride, and buffered citrate tubes be used for measurement of HbA1c on the Bio-Rad D10?
- How to reduce EDTA contamination in laboratory specimens: a Tunisian experience
- Does mean platelet volume share any relationship with biochemical and hematological parameters?
- Association of retrospective markers of glycemia and the use of continuous glucose monitoring in white adults with type 2 diabetes mellitus – a preliminary report
- Operative performances and efficiency for infectious disease testing of two immunochemistry analyzers – Abbott ARCHITECT i2000SR and DiaSorin Liaison XL
