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Increased serum homocitrulline concentrations are associated with the severity of coronary artery disease

  • Stéphane Jaisson EMAIL logo , Mohsen Kerkeni , Izabella C.R. Santos-Weiss , Faouzi Addad , Mohammed Hammami and Philippe Gillery
Published/Copyright: August 7, 2014
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Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 53 Issue 1

Abstract

Background: Carbamylation is a non-enzymatic post-translational modification of proteins that has been recently identified as a non-traditional risk factor for atherosclerosis. The aim of this study was to determine whether serum homocitrulline (HCit), a characteristic carbamylation-derived product, was related to the presence and the severity of coronary artery disease (CAD).

Methods: Forty-five control subjects and 109 patients were included in this cross-sectional study. After coronary angiography, the patients were classified as non-CAD patients (patients with normal arteries, n=33) and CAD patients (n=76). The severity of CAD was then evaluated using the Gensini scoring system. Serum total HCit concentrations were determined by LC-MS/MS.

Results: Serum HCit concentrations were significantly (p<0.001) higher in CAD patients than in control or non-CAD subjects. The receiver operating characteristic curve analysis showed an area under the curve equal to 0.908 (95% confidence interval, 0.853–0.964, p<0.001) and a threshold HCit concentration of 0.16 mmol/mol Lys for predicting the presence of CAD (78.9% sensitivity and 78.8% specificity). HCit concentrations significantly (p<0.001) increased concomitantly with the severity of CAD and were positively correlated with Gensini scores (r=0.725, p<0.001) as well as with the number of stenotic coronary arteries (p<0.001). Furthermore, in a multiple stepwise regression analysis, HCit was significantly (p<0.001) and independently associated with the presence of CAD, the Gensini score, and the number of stenotic arteries (standardized β values of 0.525, 0.722, and 0.642, respectively).

Conclusions: Our results demonstrate that serum HCit concentrations are increased during CAD and are positively associated with the severity of the disease.

Keywords: atherosclerosis; carbamylation; coronary artery disease; Gensini score; homocitrulline
Corresponding author: Dr. Stéphane Jaisson, Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, UMR CNRS/URCA no7369, 51 Rue Cognacq-Jay, 51095 Reims, France, Phone: +33-3-26-91-83-00, Fax: +33-3-26-91-80-55, E-mail:
aStéphane Jaisson and Mohsen Kerkeni participated equally in this work.

Acknowledgments

The authors thank C. Desroches and N. Leroy for technical assistance.

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Financial support: This work was supported by funds from the University of Reims Champagne-Ardenne (URCA) (France), the Centre National de la Recherche Scientifique (CNRS, France) and the “Ministère Tunisien de l’Enseignement Supérieur et de la Recherche Scientifique” (LR12ES05, University of Monastir, Tunisia). I.C.R.S.W. was the recipient of a grant from the Brazilian government (CAPES).

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2014-6-19
Accepted: 2014-7-8
Published Online: 2014-8-7
Published in Print: 2015-1-1

©2015 by De Gruyter

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https://doi.org/10.1515/cclm-2014-0642
Keywords for this article
atherosclerosis; carbamylation; coronary artery disease; Gensini score; homocitrulline

Articles in the same Issue

  1. Frontmatter
  2. Editorial
  3. Harmonisation of specialist training and continuing professional development in laboratory medicine: a long but necessary journey
  4. Reviews
  5. Laboratory medicine in the European Union
  6. Role of leptin in female reproduction
  7. Mini Review
  8. Interrelatedness between C-reactive protein and oxidized low-density lipoprotein
  9. General Clinical Chemistry and Laboratory Medicine
  10. Comparison of approaches and measurement of continuing professional development for specialists in laboratory medicine within four European countries
  11. Point-of-care urine albumin in general practice offices: effect of participation in an external quality assurance scheme
  12. Triple positive antiphospholipid antibody profile in outpatients with tests for lupus anticoagulants
  13. Effects of cigarette smoking on circulating leukocytes and plasma cytokines in monozygotic twins
  14. Proteolysis is a confounding factor in the interpretation of faecal calprotectin
  15. Urinary neutrophil gelatinase-associated lipocalin and clinical outcomes in chronic kidney disease patients
  16. Cancer Diagnostics
  17. Quantification of adult T-cell leukemia/lymphoma cells using simple four-color flow cytometry
  18. Serum fucosylated haptoglobin in chronic liver diseases as a potential biomarker of hepatocellular carcinoma development
  19. Cardiovascular Diseases
  20. Increased serum homocitrulline concentrations are associated with the severity of coronary artery disease
  21. S100B concentrations increase perioperatively in jugular vein blood despite limited metabolic and inflammatory response to clinically uneventful carotid endarterectomy
  22. Long-term biological variability of galectin-3 after heart transplantation
  23. Diabetes
  24. Evaluation of a next generation direct whole blood enzymatic assay for hemoglobin A1c on the ARCHITECT c8000 chemistry system
  25. The 13C-glucose breath test is a valid non-invasive screening tool to identify metabolic syndrome in adolescents
  26. Comparison of a direct enzymatic assay and polyacrylamide tube gel electrophoresis for measurement of small, dense low-density lipoprotein cholesterol
  27. Letters to the Editor
  28. Comparison of Abbott Architect high-sensitivity troponin I in Rapid Serum Tubes and plasma
  29. Can EDTA, EDTA-fluoride, and buffered citrate tubes be used for measurement of HbA1c on the Bio-Rad D10?
  30. How to reduce EDTA contamination in laboratory specimens: a Tunisian experience
  31. Does mean platelet volume share any relationship with biochemical and hematological parameters?
  32. Association of retrospective markers of glycemia and the use of continuous glucose monitoring in white adults with type 2 diabetes mellitus – a preliminary report
  33. Operative performances and efficiency for infectious disease testing of two immunochemistry analyzers – Abbott ARCHITECT i2000SR and DiaSorin Liaison XL
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