Startseite Molecular and morphological reappraisal of Spyridiocolax capixabus (Spyridiaceae, Rhodophyta), a rare endemic parasite from Brazil
Artikel
Lizenziert
Nicht lizenziert Erfordert eine Authentifizierung

Molecular and morphological reappraisal of Spyridiocolax capixabus (Spyridiaceae, Rhodophyta), a rare endemic parasite from Brazil

  • Daniella Harumi Chen

    Daniella Harumi Chen graduated in Biological Sciences at the Universidade Presbiteriana Mackenzie. She has worked as a technician at the Laboratory of Marine Phycology, Institute of Botany, São Paulo. She has experience in the area of Molecular Biology with emphasis on taxonomy and DNA barcoding of marine macroalgae. She received her Master’s degree in Plant Biodiversity and Environment with studies on the holotypes of Ceramiales (Rhodophyta) from Brazil housed at SP and SPF herbaria.

         , Luanda Pereira Soares

    Luanda Pereira Soares is a postdoctoral researcher at the Nucleus for Research in Phycology, Institute of Botany, São Paulo, Brazil. She received her PhD in Plant Biodiversity and Environment in 2015 at the same institution, and since then, she has been developing studies related to floristic surveys of Rhodophyta, using classical taxonomy and molecular tools. At present, her research includes taxonomy and molecular phylogeny of marine macroalgae, focusing on reassessment of type specimens, endemic and rare marine algae occurring along the Brazilian coast.

     ORCID logo     und Mutue Toyota Fujii

    Mutue Toyota Fujii is a Scientific Researcher at the Nucleus for Research in Phycology, Institute of Botany, São Paulo, Brazil. She received her Master and PhD degrees in Plant Biology from the São Paulo State University for her studies on morphological, chemical and cytogenetical approaches on Laurencia s.l. (Rhodophyta) from Brazil. Currently, she is a coordinator of the Postgraduate Program in Plant Biodiversity and Environment (PPG-IBt) and her area of activity is taxonomy, phylogeny, phylogeography and biogeography of marine macroalgae.

     ORCID logo EMAIL logo
Veröffentlicht/Copyright: 12. Juni 2019
Botanica Marina
Aus der Zeitschrift Botanica Marina Band 62 Heft 4

Abstract

The monotypic red algal parasite genus Spyridiocolax was proposed from material from the coast of Espírito Santo to accommodate Spyridiocolax capixabus, endemic from Brazil. The species is rare, and we performed a morphological reappraisal and the first molecular characterization of S. capixabus. Parasite specimens growing on Spyridia clavata were collected in the type locality, Itaoca Beach, Espírito Santo. Spyridiocolax capixabus forms colorless warts on the branches of S. clavata, and the secondary pit connections were described for the first time. Male, female and tetrasporophytic structures were observed growing in the same host plant. The plastid rbcL sequences of parasite and host were identical. A divergence of one nucleotide was found to the nuclear SSU rRNA gene, suggesting that the parasite retains the chloroplast of its host. Both plastidial and nuclear phylogenies supported the close relationship of S. capixabus and S. clavata. The data obtained corroborate other studies with rhodophycean parasites, which show morphological and molecular similarities between parasites and hosts. To maintain the monophyly of the host genus, the transfer of S. capixabus to Spyridia is proposed here on the basis of morphological and molecular evidence. Our study constitutes a starting point for reinvestigating the red algal parasites in Brazil.

Keywords: biodiversity; parasitic red algae; phylogeny; rbcL; SSU rRNA

About the authors

Daniella Harumi Chen

Daniella Harumi Chen graduated in Biological Sciences at the Universidade Presbiteriana Mackenzie. She has worked as a technician at the Laboratory of Marine Phycology, Institute of Botany, São Paulo. She has experience in the area of Molecular Biology with emphasis on taxonomy and DNA barcoding of marine macroalgae. She received her Master’s degree in Plant Biodiversity and Environment with studies on the holotypes of Ceramiales (Rhodophyta) from Brazil housed at SP and SPF herbaria.

Luanda Pereira Soares

Luanda Pereira Soares is a postdoctoral researcher at the Nucleus for Research in Phycology, Institute of Botany, São Paulo, Brazil. She received her PhD in Plant Biodiversity and Environment in 2015 at the same institution, and since then, she has been developing studies related to floristic surveys of Rhodophyta, using classical taxonomy and molecular tools. At present, her research includes taxonomy and molecular phylogeny of marine macroalgae, focusing on reassessment of type specimens, endemic and rare marine algae occurring along the Brazilian coast.

Mutue Toyota Fujii

Mutue Toyota Fujii is a Scientific Researcher at the Nucleus for Research in Phycology, Institute of Botany, São Paulo, Brazil. She received her Master and PhD degrees in Plant Biology from the São Paulo State University for her studies on morphological, chemical and cytogenetical approaches on Laurencia s.l. (Rhodophyta) from Brazil. Currently, she is a coordinator of the Postgraduate Program in Plant Biodiversity and Environment (PPG-IBt) and her area of activity is taxonomy, phylogeny, phylogeography and biogeography of marine macroalgae.

Acknowledgments

This work was partially supported by research grants from the Fundação de Amparo à Pesquisa do Estado de São Paulo – FAPESP (2016/50370-7, Funder Id: http://dx.doi.org/10.13039/501100001807) and from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq 440523/2015-0, Funder Id: http://dx.doi.org/10.13039/501100003593). DHC thanks the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil) for the MSc fellowship (CAPES/AUXPE-CIMAR 1991/2014). “This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Brasil (CAPES) Finance Code 001”. MTF thanks CNPq for the Productivity fellowship (304899/2017-8). The authors are grateful to the reviewers for their comments and suggestions.

References

Apt, K.E. and K.E. Schlech. 1998. Ululania stellata gen. et sp. nov. (Rhodomelaceae), a new genus and species of parasitic red algae from Hawaii. Phycologia 37: 157–161.10.2216/i0031-8884-37-3-157.1Suche in Google Scholar

Batters, E.A.L. 1892. Gonimophyllum buffhami: a new marine algae. J. Bot. 30: 65–67.Suche in Google Scholar

Benson, D.A., M. Cavanaugh, K. Clark, I. Karsch-Mizrachi, D.J. Lipman, J. Ostell and E.W. Sayers. 2013. GenBank. Nucleic Acids Res. 41: 36–42.10.1093/nar/gks1195Suche in Google Scholar

Blouin, N.A. and C.E. Lane. 2012. Red algal parasites: Models for a life history evolution that leaves photosynthesis behind again and again. Bioessays 34: 226–235.10.1002/bies.201100139Suche in Google Scholar

de Queiroz, K. 2012. Biological nomenclature from Linnaeus to the PhyloCode. Bibli. Herpetol. 9: 135–145.Suche in Google Scholar

de Queiroz, K. and J. Gauthier. 1992. Phylogenetic taxonomy. Annu. Rev. Ecol. Syst. 23: 449–480.10.1146/annurev.es.23.110192.002313Suche in Google Scholar

de Queiroz, K. and J. Gauthier. 1994. Toward a phylogenetics system of biological nomenclature. Trends Ecol. Evol. 9: 27–31.10.1016/0169-5347(94)90231-3Suche in Google Scholar

Feldmann, J. and G. Feldmann. 1958. Recherches sur quelques Floridées parasites. Rev. Gen. Bot. 65: 49–128.Suche in Google Scholar

Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791.10.1111/j.1558-5646.1985.tb00420.xSuche in Google Scholar PubMed

Freese, J.L. and C.E. Lane. 2017. Parasitism finds many solutions to the same problems in red algae (Florideophyceae, Rhodophyta). Mol. Biochem. Parasitol. 24: 105–111.10.1016/j.molbiopara.2017.04.006Suche in Google Scholar PubMed

Freshwater, D.W. and J. Rueness. 1994. Phylogenetic relationships of some European Gelidium (Gelidiales, Rhodophyta) species, based on rbcL nucleotide sequence analysis. Phycologia 33: 187–194.10.2216/i0031-8884-33-3-187.1Suche in Google Scholar

Fujii, M.T. and S.M.P.B. Guimarães. 1999. Morphological studies of the parasitic red alga Janczewskia moriformis (Rhodomelaceae, Ceramiales) from Brazil. Phycologia 38: 1–7.10.2216/i0031-8884-38-1-1.1Suche in Google Scholar

Goff, L.J. 1982. The biology of parasitic red algae. In: (F.E. Round and D.J. Chapman, eds) Progress in Phycological Research. Elsevier Biomedical Press, Amsterdam. pp. 289–369.Suche in Google Scholar

Goff, L.J. and A.W. Coleman. 1995. Fate of parasite and host organelle DNA during cellular-transformation of red algae by their parasites. Plant Cell 7: 1899–1911.10.2307/3870197Suche in Google Scholar

Goff, L.J., D.A. Moon, P. Nyvall, B. Stache, K. Mangin and G.C. Zuccarello. 1996. The evolution of parasitism in the red algae: molecular comparisons of aldephoparasites and their hosts. J. Phycol. 32: 297–312.10.1111/j.0022-3646.1996.00297.xSuche in Google Scholar

Goff, L.J., J. Ashen and D. Moon. 1997. The evolution of parasites from their hosts: a case study in the parasitic red algae. Evolution 51: 1068–1078.10.1111/j.1558-5646.1997.tb03954.xSuche in Google Scholar PubMed

Guimarães, S.M.P.B. 1993. Morphology and systematics of the red algal parasite Dawsoniocolax bostrychiae (Choreocolacaceae, Rhodophyta). Phycologia 32: 251–258.10.2216/i0031-8884-32-4-251.1Suche in Google Scholar

Guindon, S., J.-F. Dufayard, V. Lefort, M. Anisimova, W. Hordijk and O. Gascuel. 2010. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst. Biol. 59: 307–321.10.1093/sysbio/syq010Suche in Google Scholar PubMed

Guiry, M.D. and G.M. Guiry. 2019. AlgaeBase. World-wide electronic publication, National University of Ireland, Galway. http://www.algaebase.org.Suche in Google Scholar

Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41: 95–98.Suche in Google Scholar

Hörandl, E. 2006. Paraphyletic versus monophyletic taxa–evolutionary versus cladistic classifications. Taxon 55: 564–570.10.2307/25065631Suche in Google Scholar

Joly, A.B. 1966. Centrocerocolax, a new parasitic genus of Rhodophyta. Rickia 2: 73–77.Suche in Google Scholar

Joly, A.B. and E.C. Oliveira Filho. 1966. Spyridiocolax and Heterodasya two new genera of the Rhodophyceae. Sellowia 18: 115–125.Suche in Google Scholar

Joly, A.B. and N. Yamaguishi-Tomita. 1967. Dawsoniella bostrychiae: a new parasite of mangrove algae. Sellowia 19: 63–70.Suche in Google Scholar

Kim, M.-S. and G.-Y. Cho. 2010. A new red algal parasite, Symphyocolax koreana gen. et. sp. nov. (Rhodomelaceae, Ceramiales), from Korea. Algae 25: 105–113.10.4490/algae.2010.25.3.105Suche in Google Scholar

Kraft, G.T. and I.A. Abbott. 2002. The anatomy of Neotenophycus ichthyosteus gen. et sp. nov. (Rhodomelaceae, Ceramiales), a bizarre red algal parasite from the central Pacific. Eur. J. Phycol. 37: 269–278.10.1017/S0967026202003554Suche in Google Scholar

Kurihara, A., T. Abe, M. Tani and A.R. Sherwood. 2010. Molecular phylogeny and evolution of red algal parasites: a case study of Benzaitenia, Janczewskia, and Ululania (Ceramiales). J. Phycol. 46: 580–590.10.1111/j.1529-8817.2010.00834.xSuche in Google Scholar

Lin, S.M., S. Fredericq and M.H. Hommersand. 2001. Systematics of the Delesseriaceae (Ceramiales, Rhodophyta) based on large subunit rDNA and rbcL sequences, including the Phycodryoideae subfam. nov. J. Phycol. 37: 881–899.10.1046/j.1529-8817.2001.01012.xSuche in Google Scholar

Milne, I., D. Lindner, M. Bayer, D. Husmeier, G. McGuire, D.F. Marshall and F. Wright. 2009. TOPALi v2: a rich graphical interface for evolutionary analyses of multiple alignments on HPC clusters and multi-core desktops. Bioinformatics 25: 126–127.10.1093/bioinformatics/btn575Suche in Google Scholar PubMed PubMed Central

Ng, P.-K., P.-E. Lim and S.-M. Phang. 2014a. Radiation of the red algal parasite Congracilaria babae onto a secondary host species, Hydropuntia sp. (Gracilariaceae, Rhodophyta). PLoS One 9: e0097450.10.1371/journal.pone.0097450Suche in Google Scholar PubMed PubMed Central

Ng, P.-K., P.-E. Lim, A. Kato and S.-M. Phang. 2014b. Molecular evidence confirms the parasite Congracilaria babae (Gracilariaceae, Rhodophyta) from Malaysia. J. Appl. Phycol. 26: 1287–1300.10.1007/s10811-013-0166-5Suche in Google Scholar

Oliveira Filho, E.C. 1969. Algas marinhas do sul do estado do Espírito Santo (Brasil). I – Ceramiales. Bol. Fac. Filos. Cienc. Let. Univ. São Paulo (Botânica 26) 343: 1–277.10.11606/issn.2318-5988.v26i1p7-277Suche in Google Scholar

Oliveira Filho, E.C. and Y. Ugadim. 1973. Levringiella polysiphoniae a new species of parasitic red algae (Rhodophyta-Rhodomelaceae) from Brazil. Bol. Bot. Univ. São Paulo 1: 95–99.10.11606/issn.2316-9052.v1i0p95-99Suche in Google Scholar

Posada, D. and K.A. Crandall. 1998. MODELTEST: testing the model of DNA substitution. Bioinformatics 14: 817–818.10.1093/bioinformatics/14.9.817Suche in Google Scholar PubMed

Preuss, M. and G.C. Zuccarello. 2014. What’s in a name? Monophyly of genera in the red algae: Rhodophyllis parasitic sp. nov. (Gigartinales, Rhodophyta): a new red algal parasite from New Zealand. Algae 29: 279–288.10.4490/algae.2014.29.4.279Suche in Google Scholar

Preuss, M. and G.C. Zuccarello. 2018. Three new red algal parasites from New Zealand: Cladhymenia oblongifoliaphila sp. nov. (Rhodomelaceae), Phycodrys novae-zelandiaephila sp. nov. (Delesseriaceae) and Judithia parasitica sp. nov. (Kallymeniaceae). Phycologia 57: 9–19.10.2216/17-36.1Suche in Google Scholar

Preuss, M., W.A. Nelson and G.C. Zuccarello. 2017. Red algal parasites: a synopsis of described species, their hosts, distinguishing characters and areas for continued research. Bot. Mar. 60: 13–25.10.1515/bot-2016-0044Suche in Google Scholar

Reinsch, P.F. 1875. Contributiones ad algologiam et fungologiam. Vol. I. T.O. Welgel, Lipsiae. pp. 422.10.5962/bhl.title.49697Suche in Google Scholar

Ronquist, F., M. Teslenko, P. Van der Mark, D.L. Ayres, A. Darling, S. Höhna, B. Larget, L. Liu, M.A. Suchard and J.P. Huelsenbeck. 2012. MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Syst. Biol. 61: 539–542.10.1093/sysbio/sys029Suche in Google Scholar PubMed PubMed Central

Saunders, G.W. and G.T. Kraft. 1994. Small-subunit rRNA gene sequences from representatives of selected families of the Gigartinales and Rhodymeniales (Rhodophyta). 1. Evidence for the Plocamiales ord. nov. Can. J. Bot. 72: 1250–1263.10.1139/b94-153Suche in Google Scholar

Setchell, W.A. 1918. Parasitism among the red algae. Proc. Am. Philos. Soc. 57: 155–172.Suche in Google Scholar

Thiers, B. 2019. Index Herbariorum. A global directory of public herbaria and associated staff. New York Botanical Garden’s Virtual Herbarium. http://sweetgum.nybg.org/science/ih/.Suche in Google Scholar

Vergés, A., C. Izquierdo and M. Verlaque. 2005. Rhodymeniocolax mediterraneus sp. nov. (Rhodymeniales, Rhodophyta), parasitic on Rhodymenia ardissonei from the western Mediterranean Sea. Phycologia 44: 510–516.10.2216/0031-8884(2005)44[510:RMSNRR]2.0.CO;2Suche in Google Scholar

West, J.A. and H.P. Calumpong. 1988. Dawsoniocolax bostrychiae (Choreocolacaceae, Gigartinales), an alloparasitic red alga new to Australia. Phycologia 27: 463–468.10.2216/i0031-8884-27-4-463.1Suche in Google Scholar

Wynne, M.J. and F. Scott. 1989. Pitycolax, a new genus of adelphoparasitic red algae from lle Amsterdam, Southern Indian Ocean. Cryptogam. Algol. 10: 23–32.Suche in Google Scholar

Yoneshigue, Y. 1985. Taxonomie et ecologie des algues marines dans la region de Cabo Frio (Rio de Janeiro, Brasil). Unpublished doctoral thesis, L’Universite D’Aix-Marseille II, Merseille, France. pp. 466.Suche in Google Scholar

Yoneshigue, Y. and E.C. Oliveira Filho. 1984. Algae from Cabo Frio upwelling area. 2. Gelidiocolax pustulata (Gelidiaceae, Rhodophyta): an unusual new putative parasitic species. J. Phycol. 20: 440–443.10.1111/j.0022-3646.1984.00440.xSuche in Google Scholar

Zuccarello, G.C. and J.A. West. 1994. Host specificity on the red algal parasites Bostrychiocolax australis gen. et sp. nov. and Dawsoniocolax bostrychiae (Choreocolacaceae, Rhodophyta). J. Phycol. 30: 137–146.10.1111/j.0022-3646.1994.00137.xSuche in Google Scholar

Zuccarello, G.C., D. Moon and L.J. Goff. 2004. A phylogenetic study of parasitic genera placed in the family Choreocolacaceae (Rhodophyta). J. Phycol. 40: 937–945.10.1111/j.1529-8817.2004.04029.xSuche in Google Scholar

Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/bot-2018-0089).

Received: 2018-09-30
Accepted: 2019-04-30
Published Online: 2019-06-12
Published in Print: 2019-08-27

© 2019 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

  1. Frontmatter
  2. In this issue
  3. Physiology and ecology
  4. Application of deep learning techniques for determining the spatial extent and classification of seagrass beds, Trang, Thailand
  5. First spatial distribution of potentially toxic benthic dinoflagellates in the Lesser Antilles (Guadeloupe and Martinique), Caribbean Sea
  6. A comparative analysis of the organic and inorganic carbon content of Halimeda and Penicillus (Chlorophyta, Bryopsidales) in a coastal subtropical lagoon
  7. Taxonomy/phylogeny and biogeography
  8. Updates on Section Pseudo-opuntia of Halimeda: phylogenetic analyses of H. soniae sp. nov. (Bryopsidales, Chlorophyta) along the Brazilian coast
  9. Notes on the benthic marine algae of Puerto Rico. XII: additions to the flora
  10. Molecular and morphological reappraisal of Spyridiocolax capixabus (Spyridiaceae, Rhodophyta), a rare endemic parasite from Brazil
  11. Fourth addendum to the synoptic review of red algal genera
  12. Genomics
  13. Comparison of proteomic profiles of the phaeophyte Saccharina japonica thalli proximal to and beneath the front of epiphytic hydrozoan colonies against healthy tissue
  14. Chemistry and applications
  15. First insight into the phenolic content of Spartina maritima: isolation, characterization and quantification of four C-glycosidic flavonoids
https://doi.org/10.1515/bot-2018-0089
Schlagwörter für diesen Artikel
biodiversity; parasitic red algae; phylogeny; rbcL; SSU rRNA

Artikel in diesem Heft

  1. Frontmatter
  2. In this issue
  3. Physiology and ecology
  4. Application of deep learning techniques for determining the spatial extent and classification of seagrass beds, Trang, Thailand
  5. First spatial distribution of potentially toxic benthic dinoflagellates in the Lesser Antilles (Guadeloupe and Martinique), Caribbean Sea
  6. A comparative analysis of the organic and inorganic carbon content of Halimeda and Penicillus (Chlorophyta, Bryopsidales) in a coastal subtropical lagoon
  7. Taxonomy/phylogeny and biogeography
  8. Updates on Section Pseudo-opuntia of Halimeda: phylogenetic analyses of H. soniae sp. nov. (Bryopsidales, Chlorophyta) along the Brazilian coast
  9. Notes on the benthic marine algae of Puerto Rico. XII: additions to the flora
  10. Molecular and morphological reappraisal of Spyridiocolax capixabus (Spyridiaceae, Rhodophyta), a rare endemic parasite from Brazil
  11. Fourth addendum to the synoptic review of red algal genera
  12. Genomics
  13. Comparison of proteomic profiles of the phaeophyte Saccharina japonica thalli proximal to and beneath the front of epiphytic hydrozoan colonies against healthy tissue
  14. Chemistry and applications
  15. First insight into the phenolic content of Spartina maritima: isolation, characterization and quantification of four C-glycosidic flavonoids
Jetzt anmelden und 20% sparen
Institutioneller Zugang
Wie funktioniert Authentifizierung?
Haben Sie eine Idee, wie wir unsere Website verbessern können?
Bitte schreiben Sie uns.
© 2025 De Gruyter Brill
Heruntergeladen am 30.9.2025 von https://www.degruyterbrill.com/document/doi/10.1515/bot-2018-0089/html?lang=de
Button zum nach oben scrollen