Isolation and identification of the marine diatom Entomoneis sp. MMOGRB 0374S (Bacillariophyta): a strain with high arachidonic acid composition
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Lijuan He
Lijuan He received her PhD in 2014 at the Institute of Botany, Beijing, China. She is now a postdoctoral researcher in the Animal Husbandry and Fisheries Research Center, Guangzhou, China, and the Institute of Hydrobiology, Wuhan, China. Her research includes the taxonomy, phylogeny and utilization of microalgae.
Sulin Lou is a postdoctoral researcher in the Third Institute of Oceanography. She was awarded a PhD by South China Agricultural University, Guangzhou, China in 2013. Her main research focuses on the biochemistry and molecular biology of microalgae.
Xiangzhi Lin is a Professor in the Third Institute of Oceanography, Xiamen, China. He was awarded of a PhD degree by the Institute of Hydrobiology. His research includes the aquaculture and comprehensive utilization of marine biological resources.
Xueqiao Qian has been the Associate Professor and Technical Director in Animal Husbandry and Fisheries Research Center, Guangzhou, China since 2003. He received his PhD degree in 2001 at the Institute of Hydrobiology. He mainly studies the development of green feed.
Shouqi Xie is a Professor and Deputy Director in the Institute of Hydrobiology. He is currently working in bio-energetics and fishery studies.
Zhaokai Wang is a research assistant in the Third Institute of Oceanography. He obtained his PhD degree from Fujian Normal University. His research focuses on marine biology.
Abstract
A unicellular marine diatom, Entomoneis sp. MMOGRB 0374S, was isolated as a candidate strain for high production of a polyunsaturated fatty acid (PUFA), arachidonic acid (ARA, 20:4ω6). Its morphological characters were typical for representatives of the genus Entomoneis, e.g. junction line, sigmoid keel and panduriform girdle view. This species was differentiated from other Entomoneis species by its smaller size and denser stria. Phylogenetically, it was distinct from other taxa with a long branch. This relationship was also emphasized by only 94% nucleotide similarity between Entomoneis sp. MMOGRB 0374S and other related species. The biomass concentration reached 182 mg l−1 at the stationary phase and 229 mg l−1 after 3 days of nitrogen-limited culture. Lipids were extracted using four methods, and the dichloromethane (DCM) /methanol method was the most effective, yielding 36% of dry biomass at the stationary phase and 40% after 3 days of nitrogen-free culture. Gas chromatography revealed 17 types of fatty acids. ARA was the major component of PUFA and represented between 16% and 19% of the total fatty acids using the four lipid extraction methods, among which DCM/methanol yielded the highest proportion of ARA. Considering the yields of both total lipids and ARA, the DCM/methanol method was the most effective for ARA extraction.
About the authors
Lijuan He received her PhD in 2014 at the Institute of Botany, Beijing, China. She is now a postdoctoral researcher in the Animal Husbandry and Fisheries Research Center, Guangzhou, China, and the Institute of Hydrobiology, Wuhan, China. Her research includes the taxonomy, phylogeny and utilization of microalgae.
Sulin Lou is a postdoctoral researcher in the Third Institute of Oceanography. She was awarded a PhD by South China Agricultural University, Guangzhou, China in 2013. Her main research focuses on the biochemistry and molecular biology of microalgae.
Xiangzhi Lin is a Professor in the Third Institute of Oceanography, Xiamen, China. He was awarded of a PhD degree by the Institute of Hydrobiology. His research includes the aquaculture and comprehensive utilization of marine biological resources.
Xueqiao Qian has been the Associate Professor and Technical Director in Animal Husbandry and Fisheries Research Center, Guangzhou, China since 2003. He received his PhD degree in 2001 at the Institute of Hydrobiology. He mainly studies the development of green feed.
Shouqi Xie is a Professor and Deputy Director in the Institute of Hydrobiology. He is currently working in bio-energetics and fishery studies.
Zhaokai Wang is a research assistant in the Third Institute of Oceanography. He obtained his PhD degree from Fujian Normal University. His research focuses on marine biology.
Acknowledgments
This study was supported by Public Science and Technology Research Funds Projects of Ocean (201305022), Special Project of Scientific Research Foundation of Third Institute of Oceanography (2009052), Xiamen Southern Oceanographic Center (14CZP028HJ02) and Special Project of Ministry of Science and Technology (2012EG149243). The authors wish to thank Keigo Osada for his help in taxonomic identification. Jiaqi Huang and Huanqing Li are also appreciated for performing the analysis of the fatty acid samples.
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©2016 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
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- In this issue
- Review
- Third addendum to the synoptic review of red algal genera
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- Isolation and identification of the marine diatom Entomoneis sp. MMOGRB 0374S (Bacillariophyta): a strain with high arachidonic acid composition
- Reproductive phenology of the subtropical seagrasses Thalassia testudinum (turtle grass) and Halodule wrightii (shoal grass) in the northwest Gulf of Mexico
- Short communication
- Macroscopic sexual dimorphism in Fucus radicans (Phaeophyceae) with implications for its reproductive ecology
- Annual reviewer acknowledgement
- Reviewer acknowledgement Botanica Marina volume 59 (2016)
Articles in the same Issue
- Frontmatter
- In this issue
- Review
- Third addendum to the synoptic review of red algal genera
- Research articles
- Transfer of Papenfussia japonica (Delesseriaceae, Rhodophyta) from Korea to the genus Augophyllum
- The genus Ramicrusta (Peyssonneliales, Rhodophyta) in the Caribbean Sea, including Ramicrusta bonairensis sp. nov. and Ramicrusta monensis sp. nov.
- Sorus formation on the holdfast haptera of the kelp Ecklonia radicosa (Phaeophyceae, Laminariales)
- Codium fragile in Norway: subspecies identity and morphology
- Fate of two invasive or potentially invasive alien seaweeds in a central Mediterranean transitional water system: failure and success
- Isolation and identification of the marine diatom Entomoneis sp. MMOGRB 0374S (Bacillariophyta): a strain with high arachidonic acid composition
- Reproductive phenology of the subtropical seagrasses Thalassia testudinum (turtle grass) and Halodule wrightii (shoal grass) in the northwest Gulf of Mexico
- Short communication
- Macroscopic sexual dimorphism in Fucus radicans (Phaeophyceae) with implications for its reproductive ecology
- Annual reviewer acknowledgement
- Reviewer acknowledgement Botanica Marina volume 59 (2016)
