Ribosomal intergenic spacer (IGS) sequence can distinguish varieties of Pyropia yezoensis cultivated in China
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Yuan He
Yuan He received a Master’s of Science degree from SuZhou University of Science and Technology, Jiangsu China in 2013. Since then he is working in the lab of Professor Songdong Shen. He is mainly engaged in the study of molecular biology and taxonomy of algae.
Jiajie Xu received a Master’s of Science degree from SuZhou University of Science and Technology, Jiangsu, China in 2012. Between 2012 and now she worked in the lab of Professor Songdong Shen. She is working in a school as a teacher. She is mainly engaged in breeding and classification of seaweeds.
Xingchen Li received a Master’s of Science degree at Fuyang Teachers College, Anhui China in 2011. Between 2011 and 2014, she worked in the lab of Professor Songdong Shen. She is working in Soochow University as a lecturer. Her major tasks include cell development of algae and molecular mechanisms of plants.
Songdong Shen is a phycology researcher mainly focusing on cell and molecular biology of
Pyropia and ofUlva prolifera , which caused the largest green tides recently in China. He has studied reproductive diversity, growth factors, species identification and phylogeny in order to find the source and the cause.
,
Jianyi Zhu
Jianyi Zhu has been engaged in the study of the breeding and cultivation of
Pyropia for over 30 years. She is the director of Chinese NationalPyropia Germplasm Repository. She has cultivated new strains ofPyropia yezoensis in recent years.Zonggen Shen received a PhD in 2001 from the Institute of Botany in Northwest University in Xi’an, China, where he studied Plant Physiology. Since 2003, he has been a research botanist with the Changshu Institute of Technology in China. His team is mainly engaged in the study of plant cytology and structural botany.
Bo Jiang received a Master’s degree in Botany in 2009. Since 2014, he has been studying for a PhD at the Institute of Oceanology, Chinese Academy of Sciences. He is mainly engaged in the study of phycology and botany.
Qinqin Lu received a Master’s of Science degree from the Nanjing Agricultural University in China. He is the director of the Institute of Oceanology and Marine Fisheries of Jiangsu province in China. His studies focus on the development of the
Pyropia industry.
Abstract
The ribosomal intergenic spacer (IGS) variation was compared for the nine main cultivated varieties of Pyropia yezoensis in China obtained from the Chinese National Pyropia Germplasm Repository. The full length of the IGS is 5984 bp including several sequence repeats and some complex structures. The sequence analysis of four partial IGS amplicons ranged from 3628 bp to 3776 bp; and the G+C content is 52.5%. Through similarity analysis and multiple sequence alignment of these partial IGS amplicons from the nine varieties, we have found 278 variable sites which account for approximately 7.5% of the whole IGS length. Both comparison of partial IGS sequences and phylogenetic analysis indicate high levels of genetic variation among different P. yezoensis cultivars in different areas of China. In comparison to the IGS of P. haitanensis, this study highlights the important function of classification of IGS in P. yezoensis. Therefore, the IGS sequence may be used to identify intraspecific variation in P. yezoensis.
About the authors
Yuan He received a Master’s of Science degree from SuZhou University of Science and Technology, Jiangsu China in 2013. Since then he is working in the lab of Professor Songdong Shen. He is mainly engaged in the study of molecular biology and taxonomy of algae.
Jiajie Xu received a Master’s of Science degree from SuZhou University of Science and Technology, Jiangsu, China in 2012. Between 2012 and now she worked in the lab of Professor Songdong Shen. She is working in a school as a teacher. She is mainly engaged in breeding and classification of seaweeds.
Xingchen Li received a Master’s of Science degree at Fuyang Teachers College, Anhui China in 2011. Between 2011 and 2014, she worked in the lab of Professor Songdong Shen. She is working in Soochow University as a lecturer. Her major tasks include cell development of algae and molecular mechanisms of plants.
Songdong Shen is a phycology researcher mainly focusing on cell and molecular biology of Pyropia and of Ulva prolifera, which caused the largest green tides recently in China. He has studied reproductive diversity, growth factors, species identification and phylogeny in order to find the source and the cause.
Jianyi Zhu has been engaged in the study of the breeding and cultivation of Pyropia for over 30 years. She is the director of Chinese National Pyropia Germplasm Repository. She has cultivated new strains of Pyropia yezoensis in recent years.
Zonggen Shen received a PhD in 2001 from the Institute of Botany in Northwest University in Xi’an, China, where he studied Plant Physiology. Since 2003, he has been a research botanist with the Changshu Institute of Technology in China. His team is mainly engaged in the study of plant cytology and structural botany.
Bo Jiang received a Master’s degree in Botany in 2009. Since 2014, he has been studying for a PhD at the Institute of Oceanology, Chinese Academy of Sciences. He is mainly engaged in the study of phycology and botany.
Qinqin Lu received a Master’s of Science degree from the Nanjing Agricultural University in China. He is the director of the Institute of Oceanology and Marine Fisheries of Jiangsu province in China. His studies focus on the development of the Pyropia industry.
Acknowledgments
This work was supported by National Natural Science Foundation of China (Project no. 31270256, No. 31272664) and National “863” Project (Project No. 2012 AA10A 406-6).
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©2015 by De Gruyter
Artikel in diesem Heft
- Frontmatter
- In this issue
- Research articles
- Food or fad? Challenges and opportunities for including seaweeds in a Nordic diet
- Seasonal and spatial variation in biochemical composition of Saccharina latissima during a potential harvesting season for Western Sweden
- European aquaculture of sugar kelp (Saccharina latissima) for food industries: iodine content and epiphytic animals as major problems
- Improved growth performance of Gracilariopsis heteroclada via short-term nitrogen enrichment
- Ribosomal intergenic spacer (IGS) sequence can distinguish varieties of Pyropia yezoensis cultivated in China
- Phylogeny and morphology of Parviphycus myriocladus (Børgesen) comb. nov. (Gelidiales, Rhodophyta) from Asian waters
- New record of Hypnea flexicaulis in New Zealand and description of Calliblepharis psammophilus sp. nov.
- On the occurrence of three red algal species new to the Mediterranean Sea in Al-Hoceima National Park (Morocco)
- The South African estuarine specialist Codium tenue (Bryopsidales, Chlorophyta) discovered in a south-western Australian estuary
- Morphological update on Calycina marina (Pezizellaceae, Helotiales, Leotiomycetes), a new combination for Laetinaevia marina
- Annual reviewer acknowledgement
- Reviewer acknowledgement Botanica Marina volume 58 (2015)
Artikel in diesem Heft
- Frontmatter
- In this issue
- Research articles
- Food or fad? Challenges and opportunities for including seaweeds in a Nordic diet
- Seasonal and spatial variation in biochemical composition of Saccharina latissima during a potential harvesting season for Western Sweden
- European aquaculture of sugar kelp (Saccharina latissima) for food industries: iodine content and epiphytic animals as major problems
- Improved growth performance of Gracilariopsis heteroclada via short-term nitrogen enrichment
- Ribosomal intergenic spacer (IGS) sequence can distinguish varieties of Pyropia yezoensis cultivated in China
- Phylogeny and morphology of Parviphycus myriocladus (Børgesen) comb. nov. (Gelidiales, Rhodophyta) from Asian waters
- New record of Hypnea flexicaulis in New Zealand and description of Calliblepharis psammophilus sp. nov.
- On the occurrence of three red algal species new to the Mediterranean Sea in Al-Hoceima National Park (Morocco)
- The South African estuarine specialist Codium tenue (Bryopsidales, Chlorophyta) discovered in a south-western Australian estuary
- Morphological update on Calycina marina (Pezizellaceae, Helotiales, Leotiomycetes), a new combination for Laetinaevia marina
- Annual reviewer acknowledgement
- Reviewer acknowledgement Botanica Marina volume 58 (2015)
