DNA barcoding reveals high diversity in the Gelidiales of the Brazilian southeast coast
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Cíntia Iha
Cíntia Iha is a PhD student fellow at the Universidade de São Paulo. She was awarded a Master’s degree in Botany, with an emphasis in Phycology, by the Universidade de São Paulo for her work on phylogeny and taxonomy using molecular and morphological data of Gelidiales from the Brazilian southeast coast. Her more recent research has focused on phylogenomics of Gracilariales. She is interested in the evolution and diversity of Rhodophyta.
,
Daniela Milstein
Daniela Milstein is currently an Adjunct Professor at the Department of Marine Sciences (DCMar), Federal University of São Paulo (UNIFESP), Brazil. She was awarded a Master’s and a PhD degree in Botany from the University of São Paulo, Brazil, and a postdoctoral degree from the University of New Brunswick, Canada. Her research interests are molecular systematics, phylogeny and phylogeography of marine macroalgae.
Silvia Maria P.B. Guimarães is an Associate Researcher at the Instituto de Botânica, São Paulo, Brazil. She received her Master’s degree and her PhD degree in Botany from the University of São Paulo for her work on the taxonomy of marine algae, mainly Rhodophyta. Presently, she works in the following areas: taxonomy of marine algae, algae of the infralittoral region and algae associated with rhodolith beds.
D. Wilson Freshwater is a researcher at the Center for Marine Science, University of North Carolina at Wilmington. His current research interests include molecular phylogeny and taxonomy of marine algae, application of DNA barcoding approaches in marine floristics, and ecology of marine hard bottom communities.
Mariana Cabral Oliveira is an Associate Professor at the University of São Paulo (USP), Brazil. She was awarded a PhD in Botany by USP for her work on the molecular phylogeny of Bangiales. Her main expertise is in molecular phylogeny and taxonomy of algae, and in the genomics of algae and bacteria, focusing mainly on red algae. She is interested in the origin, evolution and diversity of Rhodophyta. Presently, she integrates the coordination committee of the Biota-FAPESP program and is the head of the Botany Department at USP.
Abstract
Morphological identification of species in the order Gelidiales can be difficult and controversial because of phenotypic plasticity, the low numbers of reproductive specimens and poorly defined taxonomic boundaries. A DNA barcoding survey of Brazilian specimens of Gelidiales, employing neighbor-joining and Automatic Barcode Gap Discovery analyses, indicated the presence of 23 statistically robust primary species hypotheses (PSH). In addition to the cytochrome oxidase I gene (COI-5P), the chloroplast universal plastid amplicon (UPA) marker was also sequenced and submitted to the same analyses. Representatives of each COI-5P/UPA PSH were selected for rbcL sequence analysis to further corroborate the occurrence of 23 species and to infer their phylogenetic relationships. These analyses confirmed the identity of six species previously cited for Brazil: Gelidiella acerosa, G. ligulata, Gelidium crinale, G. floridanum, Pterocladiella bartlettii and P. capillacea. Three new reports for Brazil were also detected: Gelidium microdonticum, Pterocladiella beachiae and P. australafricanensis. Fourteen species remain unidentified and require detailed morphological evaluation.
About the authors
Cíntia Iha is a PhD student fellow at the Universidade de São Paulo. She was awarded a Master’s degree in Botany, with an emphasis in Phycology, by the Universidade de São Paulo for her work on phylogeny and taxonomy using molecular and morphological data of Gelidiales from the Brazilian southeast coast. Her more recent research has focused on phylogenomics of Gracilariales. She is interested in the evolution and diversity of Rhodophyta.
Daniela Milstein is currently an Adjunct Professor at the Department of Marine Sciences (DCMar), Federal University of São Paulo (UNIFESP), Brazil. She was awarded a Master’s and a PhD degree in Botany from the University of São Paulo, Brazil, and a postdoctoral degree from the University of New Brunswick, Canada. Her research interests are molecular systematics, phylogeny and phylogeography of marine macroalgae.
Silvia Maria P.B. Guimarães is an Associate Researcher at the Instituto de Botânica, São Paulo, Brazil. She received her Master’s degree and her PhD degree in Botany from the University of São Paulo for her work on the taxonomy of marine algae, mainly Rhodophyta. Presently, she works in the following areas: taxonomy of marine algae, algae of the infralittoral region and algae associated with rhodolith beds.
D. Wilson Freshwater is a researcher at the Center for Marine Science, University of North Carolina at Wilmington. His current research interests include molecular phylogeny and taxonomy of marine algae, application of DNA barcoding approaches in marine floristics, and ecology of marine hard bottom communities.
Mariana Cabral Oliveira is an Associate Professor at the University of São Paulo (USP), Brazil. She was awarded a PhD in Botany by USP for her work on the molecular phylogeny of Bangiales. Her main expertise is in molecular phylogeny and taxonomy of algae, and in the genomics of algae and bacteria, focusing mainly on red algae. She is interested in the origin, evolution and diversity of Rhodophyta. Presently, she integrates the coordination committee of the Biota-FAPESP program and is the head of the Botany Department at USP.
Acknowledgments
We are grateful for the support from FAPESP (2011/04224-5, 2012/03592-3, 2013/11833-3), CNPq (Br BOL-564945-2010-2, 301491/2013-5) and NP-BioMar. DWF’s work on this project was supported by NSF grant DEB-0742437 and the CMS DNA Algal Trust. We also acknowledge the detailed comments of three anonymous reviewers and Prof. Dring that greatly improved the original manuscript.
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Articles in the same Issue
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- In this issue
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- Comparison of non-native dwarf eelgrass (Zostera japonica) and native eelgrass (Zostera marina) distributions in a northeast Pacific estuary: 1997–2014
- The elusive genus Olifantiella (Bacillariophyta): South Pacific assemblage and Indo-Pacific biogeography
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- Identification, characterization and quantitative analysis of NAD-malate dehydrogenase from the marine rhodophyte Pyropia haitanensis
- DNA barcoding reveals high diversity in the Gelidiales of the Brazilian southeast coast
- Checklist of the marine macroalgae of Iran
- Short communications
- First reports of the red alga Hypoglossum caloglossoides from the Mediterranean and the Red Sea
- First report of the red alga Gayliella fimbriata in the Mediterranean Sea
Articles in the same Issue
- Frontmatter
- In this issue
- Research articles
- Comparison of non-native dwarf eelgrass (Zostera japonica) and native eelgrass (Zostera marina) distributions in a northeast Pacific estuary: 1997–2014
- The elusive genus Olifantiella (Bacillariophyta): South Pacific assemblage and Indo-Pacific biogeography
- Does bryozoan colonization alter the biochemical composition of Saccharina japonica affecting food safety and quality?
- Growth, reproduction, and senescence of the epiphytic marine alga Phaeosaccion collinsii Farlow (Ochrophyta, Phaeothamniales) at its type locality in Nahant, Massachusetts, USA
- Identification, characterization and quantitative analysis of NAD-malate dehydrogenase from the marine rhodophyte Pyropia haitanensis
- DNA barcoding reveals high diversity in the Gelidiales of the Brazilian southeast coast
- Checklist of the marine macroalgae of Iran
- Short communications
- First reports of the red alga Hypoglossum caloglossoides from the Mediterranean and the Red Sea
- First report of the red alga Gayliella fimbriata in the Mediterranean Sea
