Preparation, characterization and blood compatibility assessment of a novel electrospun nanocomposite comprising polyurethane and ayurvedic-indhulekha oil for tissue engineering applications
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Manikandan Ayyar
und
Rajasekar Rathanasamy
Abstract
Electrospun polyurethane based nanocomposite scaffolds were fabricated by mixing with indhulekha oil. Scanning electron microscope (SEM) portrayed the nanofibrous nature of the composite and the average diameters of the composite scaffold were smaller than the pristine scaffolds. The fabricated scaffold was found to be hydrophobic (114°) due to the inclusion of indhulekha oil, which was displayed in contact angle measurement analysis. The fourier transform infrared spectroscopy (FTIR) results indicated that the indhulekha oil was dispersed in PU matrix identified by formation of hydrogen bond and peak shifting of CH group. The PU/indhulekha oil nanocomposite exhibits a higher decomposition onset temperature and also residual weight percentage at 900°C was more compared to the pure PU. Surface roughness was found to be increased in the composite compared to the pristine PU as indicated by the atomic force microscopy (AFM) analysis. In order to investigate the blood compatibility of electrospun nanocomposites the activated partial thromboplastin time (APTT) assay, prothrombin time (PT) assay and hemolytic assay were performed. The blood compatibility results APTT and PT revealed that the developed nanocomposites demonstrated delayed clotting time indicating the anticoagulant nature of the composite in comparison with the pristine PU. Further, it was also observed that the hemolytic index of nanocomposites was reduced compared to pure PU suggesting the non-hemolytic nature of the fabricated scaffold. Hence, the fabricated nanocomposites might be considered as a potent substitute for scaffolding damaged tissue due to their inherent physicochemical and blood compatibility properties.
Author Statement
Research funding: This work was supported by the Ministry of Higher Education Malaysia with the Grant no. Q.J130000.2545.14H59.
Conflict of interest: Authors state no conflict of interest.
Informed consent: Informed consent is not applicable.
Ethical approval: The conducted research is not related to either human or animals use.
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©2018 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
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- Editorial
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- Electro-spun PLA-PEG-yarns for tissue engineering applications
- Preparation, characterization and blood compatibility assessment of a novel electrospun nanocomposite comprising polyurethane and ayurvedic-indhulekha oil for tissue engineering applications
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Artikel in diesem Heft
- Frontmatter
- Editorial
- The role of textile engineering in regenerative medicine
- Research articles
- Fibrous composite material for textile heart valve design: in vitro assessment
- Electro-spun PLA-PEG-yarns for tissue engineering applications
- Preparation, characterization and blood compatibility assessment of a novel electrospun nanocomposite comprising polyurethane and ayurvedic-indhulekha oil for tissue engineering applications
- Three-dimensional bioglass-collagen-phosphatidylserine scaffolds designed with functionally graded structure and mechanical features
- Differential mineralization of human dental pulp stem cells on diverse polymers
- Heart valves from polyester fibers: a preliminary 6-month in vivo study
- Adaptation of cardiovascular system stent implants
- Synthesizing selenium- and silver-substituted hydroxyapatite-based bone grafts and their effects on antibacterial efficiency and cell viability
- Long-term recording performance and biocompatibility of chronically implanted cylindrically-shaped, polymer-based neural interfaces
- Morphology and contractile gene expression of adipose-derived mesenchymal stem cells in response to short-term cyclic uniaxial strain and TGF-β1
- A novel ceramic tibial component is as safe as its metal counterpart
- Short communication
- Hybrid textile heart valve prosthesis: preliminary in vitro evaluation
