Home Effect of steroidal saponins-loaded nano-bioglass/phosphatidylserine/collagen bone substitute on bone healing
Article
Licensed
Unlicensed Requires Authentication

Effect of steroidal saponins-loaded nano-bioglass/phosphatidylserine/collagen bone substitute on bone healing

  • Chunrong Yang EMAIL logo , Huazhong Wu and Jianhua Wang
Published/Copyright: November 10, 2016

Abstract

The objective of this study was to investigate the therapeutic potential of nano-bioglass/phosphatidylserine/collagen (nBG/PS/COL) scaffolds loaded with steroidal saponins as an inducer factor for skeletal defects. The drugs-encapsulated bone substitute was prepared by loading steroidal saponins-collagen microsphere suspension in nano-bioglass and phosphatidylserine (PS) composite. The scaffolds possess an interconnected porous structure with a porosity of about 82.3%. The pore size ranges from several micrometers up to about 400 μm. The drug release assays showed the long-term sustained release of steroidal saponins from the scaffolds with effective and safe bioactivity. Moreover, in vitro and in vivo studies showed that the involvement of steroidal saponins contributed to the secretion of nerve growth factor (NGF) in MC3T3-E1 cells, which may be the possible factor that greatly enhanced bone healing. The results suggest that the bone substitute is an effective implantable drug-delivery system for use in bone repair.

Acknowledgments

This work was financially supported by natural science foundation project of Fujian (Grant no. 2014J05054), scientific research development foundation project of Fujian university of technology (Grant no. GY-Z15093), and scientific research starting foundation project of Fujian university of technology (Grant no. GY-Z0854).

References

[1] Chen SY, Qin JJ, Wang L, et al. Different effects of implanting vascular bundles and sensory nerve tracts on the expression of neuropeptide receptors in tissue-engineered bone in vivo. Biomed Mater 2010; 5: 055002.10.1088/1748-6041/5/5/055002Search in Google Scholar PubMed

[2] Cohen G, Ettinger K, Lecht S, et al. Transcriptional Down-regulation of epidermal growth factor (EGF) receptors by nerve growth factor (NGF) in PC12 cells. J Mol Neurosci 2014; 54: 574–585.10.1007/s12031-014-0388-2Search in Google Scholar PubMed

[3] Goel SC, Singh D, Rastogi A, et al. Role of tricalcium phosphate implant in bridging the large osteoperiosteal gaps in rabbits. Indian J Exp Biol 2013; 51: 375–380.Search in Google Scholar PubMed

[4] Grills BL, Schuijers JA. Immunohistochemical localization of nerve growth factor in fractured and unfractured rat bone. Acta Orthop Scand 1998; 69: 415–419.10.3109/17453679808999059Search in Google Scholar PubMed

[5] Grills BL, Schuijers JA, Ward AR. Topical application of nerve growth factor improves fracture healing in rats. J Orthop Res 1997; 15: 235–242.10.1002/jor.1100150212Search in Google Scholar PubMed

[6] Imai S, Matsusue Y. Neuronal regulation of bone metabolism and anabolism: calcitonin gene-related peptide-, substance P-, and tyrosine hydroxylase-containing nerves and the bone. Microsc Res Tech 2002; 58: 61–69.10.1002/jemt.10119Search in Google Scholar PubMed

[7] Kang BJ, Kim Y, Lee SH, et al. Collagen I gel promotes omogenous osteogenic differentiation of adipose issue-derivedmesenchymal stem cells in serum-derived albumin scaffold. J Biomater Sci Polym Ed 2013; 24: 1233–1243.10.1080/09205063.2012.745717Search in Google Scholar

[8] Lerner UH, Persson E. Osteotropic effects by the neuropeptides calcitonin gene-related peptide, substance P and vasoactive intestinal peptide. J Musculoskelet Neuronal Interact 2008; 8: 154–165.Search in Google Scholar PubMed

[9] Lucas PA, Laurencin C, Syftestad GT, et al. Ectopic induction of cartilage and bone by water-soluble proteins from bovine bone using a polyanhydride delivery vehicle. J Biomed Mater Res 1990; 24: 901–911.10.1002/jbm.820240708Search in Google Scholar PubMed

[10] Miller MR, Kasahara M. Observations of the innervation of human long bones. Anat Rec 1963; 145: 13–23.10.1002/ar.1091450104Search in Google Scholar

[11] Mogi M, Kondo A, Kinpara K, et al. Anti-apoptotic action of nerve growth factor in mouse osteoblastic cell line. Life Sci 2000; 67: 1197–1206.10.1016/S0024-3205(00)00705-0Search in Google Scholar PubMed

[12] Nakanishi T, Ohyama K, Aoki C, et al. Expression of trkc in a mouse osteoblastic cell line and its response to neurothophin-3. Biochem Biophys Res Commun 1994; 203: 1268–1274.10.1006/bbrc.1994.2319Search in Google Scholar PubMed

[13] Nilsson M, Zheng MH, Tägil M. The composite of hydroxyapatite and calcium sulphate: a review of preclinical evaluation and clinical applications. Expert Rev Med Devices 2013; 10: 675–684.10.1586/17434440.2013.827529Search in Google Scholar PubMed

[14] Qu Y, Zhang Y, Pei L, et al. New neuritogenic steroidal saponin from Ophiopogon japonicus (Thunb.) Ker-Gawl. Bioscience, Biotechnology, and Biochemistry 2011; 75: 1201–1204.10.1271/bbb.110066Search in Google Scholar PubMed

[15] Serre CM, Farlay D, Delmas PD, et al. Evidence for a dense and intimate innervation of the bone tissue, including glutamate-containing fibers. Bone 1999; 25: 623–629.10.1016/S8756-3282(99)00215-XSearch in Google Scholar PubMed

[16] Wang YZ, Wang JJ, Liang JC, et al. Effects of diosgenin on cell proliferation, differentiation and OPG/RANKL mRNA expression of rat osteoblasts cultured in vitro. China J Tra Chinese Med Pharm 2010; 25: 134–136.Search in Google Scholar

[17] Yang CR, Wang JH. Preparation and characterization of collagen microspheres for sustained release of steroidal saponins. Mater Res 2014; 17: 1644–1650.10.1590/1516-1439.307214Search in Google Scholar

[18] Yang CR, Wang YJ, Chen XF. Mineralization regulation and biological influence of bioactive glass-collagen-phosphatidylserine composite scaffolds. Sci China Life Sci 2012; 55: 236–240.10.1007/s11427-012-4291-1Search in Google Scholar PubMed

[19] Ye Y, Qu Y, Tang RQ, et al. Three new neuritogenic steroidal saponins from Ophiopogon japonicus (Thunb.) Ker-Gawl Steroids 2013; 78: 1171–1176.10.1016/j.steroids.2013.08.005Search in Google Scholar PubMed

Received: 2016-7-3
Accepted: 2016-10-6
Published Online: 2016-11-10
Published in Print: 2017-10-26

©2017 Walter de Gruyter GmbH, Berlin/Boston

Downloaded on 24.9.2025 from https://www.degruyterbrill.com/document/doi/10.1515/bmt-2016-0151/html
Scroll to top button