Individual construction of freeform-fabricated polycaprolactone scaffolds for osteogenesis

Lu Liu; Guixin Shi; Ying Cui; Hui Li; Zhengchao Li; Qiangcheng Zeng; Yong Guo

doi:10.1515/bmt-2016-0005

Article

Individual construction of freeform-fabricated polycaprolactone scaffolds for osteogenesis

Lu Liu , Guixin Shi , Ying Cui , Hui Li , Zhengchao Li , Qiangcheng Zeng and Yong Guo

Published/Copyright: January 11, 2017

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From the journal Biomedical Engineering / Biomedizinische Technik Volume 62 Issue 5

Abstract

The construction of engineered bone mostly focuses on simulating the extracellular matrix (ECM) for proper biological activity. However, the complexity of architecture and the variability of the mechanical properties of natural bones are related to individual differences in age, nutritional state, mechanical loading and disease status. Defect substitutions should be normed with the host natural bone, balancing architectural and mechanical adaption, as well as biological activity. Using a freeform fabrication (FFF) method, we prepared polycaprolactone (PCL) scaffolds with different architectures. With simulation of structural and mechanical parameters of rabbit femur cancellous bone, individual defect substitution with the characteristics of the rabbit femur was obtained with high porosity and connectivity. Biological adaption in vitro was examined and osteoid formation in vivo was assessed by implantation in situ. Simulating the femur cancellous bone, 300-μm FFF PCL scaffolds had better architectural and mechanical properties. The protocol produced an architecturally, mechanically and biologically adaptive construction of an individual model for rapid-prototype PCL scaffolds. A guide system was developed to accurately reproduce virtually individual defect substitutions of the bone.

Keywords: bone scaffold; orthotopic transplantation; polycaprolactone; rapid prototype

Corresponding author: Yong Guo, Doctor, Professor, College of Biotechnology, Guilin Medical University, Guilin 541100, Guangxi, China

aLu Liu and Guixin Shi: These authors contributed equally to this work.

Funding/support: This work was supported by National Nature Science Foundation of China (No. 11302262, 11172062).
Conflict of interest statement: The manuscript is an original work and has not been submitted or is under consideration for publication in another journal. We confirm that all the listed authors have participated actively in the study, and have seen and approved the submitted manuscript. The authors do not have any possible conflicts of interest.

References

[1] Chappard D, Basle M-F, Legrand E, Audran M. Trabecular bone microarchitecture: a review. Morphologie 2008; 92: 162–170.10.1016/j.morpho.2008.10.003Search in Google Scholar PubMed

[2] Coelho P, Fernandes P, Rodrigues H, Cardoso J, Guedes J. Numerical modeling of bone tissue adaptation – a hierarchical approach for bone apparent density and trabecular structure. J Biomech 2009; 42: 830–837.10.1016/j.jbiomech.2009.01.020Search in Google Scholar PubMed

[3] Dias MR, Guedes JM, Flanagan CL, Hollister SJ, Fernandes PR. Optimization of scaffold design for bone tissue engineering: a computational and experimental study. Med Eng Phys 2014; 36: 448–457.10.1016/j.medengphy.2014.02.010Search in Google Scholar PubMed

[4] Gabriel M, Nazmi K, Dahm M, Zentner A, Vahl C-F, Strand D. Covalent RGD modification of the inner pore surface of polycaprolactone scaffolds. J Biomater Sci Polym Ed 2012; 23: 941–953.10.1163/092050611X566793Search in Google Scholar PubMed

[5] Guo Y, Tran RT, Xie D, et al. Citrate-based biphasic scaffolds for the repair of large segmental bone defects. J Biomed Mater Res A 2015; 103: 772–781.10.1002/jbm.a.35228Search in Google Scholar PubMed PubMed Central

[6] Hassani B, Hinton E. Homogenization and structural topology optimization: theory, practice and software, London: Springer-Verlag 2012.Search in Google Scholar

[7] Heid CA, Stevens J, Livak KJ, Williams PM. Real time quantitative PCR. Genome Res. 1996; 6: 986–994.10.1101/gr.6.10.986Search in Google Scholar PubMed

[8] Ho J, Wu J, Huang H, Chen MY, Fuh L, Hsu J. Trabecular bone structural parameters evaluated using dental cone-beam computed tomography: cellular synthetic bones. Biomed Eng Online 2013; 12: 115.10.1186/1475-925X-12-115Search in Google Scholar PubMed PubMed Central

[9] Jung MR, Shim IK, Chung HJ, et al. Local BMP-7 release from a PLGA scaffolding-matrix for the repair of osteochondral defects in rabbits. J Control Release 2012; 162: 485–491.10.1016/j.jconrel.2012.07.040Search in Google Scholar PubMed

[10] Karageorgiou V, Kaplan D. Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials 2005; 26: 5474–5491.10.1016/j.biomaterials.2005.02.002Search in Google Scholar PubMed

[11] Khorasani AM, Gibson I, Goldberg M, Littlefair G. A survey on mechanisms and critical parameters on solidification of selective laser melting during fabrication of Ti-6Al-4V prosthetic acetabular cup. Mater Des 2016; 103: 348–355.10.1016/j.matdes.2016.04.074Search in Google Scholar

[12] Kumar G, Tison CK, Chatterjee K, et al. The determination of stem cell fate by 3D scaffold structures through the control of cell shape. Biomaterials 2011; 32: 9188–9196.10.1016/j.biomaterials.2011.08.054Search in Google Scholar PubMed PubMed Central

[13] Lee YS, Choi EM. Apocynin stimulates osteoblast differentiation and inhibits bone-resorbing mediators in MC3T3-E1 cells. Cell Immunol 2011; 270: 224–229.10.1016/j.cellimm.2011.05.011Search in Google Scholar PubMed

[14] Lichte P, Pape H, Pufe T, Kobbe P, Fischer H. Scaffolds for bone healing: concepts, materials and evidence. Injury 2011; 42: 569–573.10.1016/j.injury.2011.03.033Search in Google Scholar PubMed

[15] Lieb E, Vogel TS, Dauner M, Schulz MB. Effects of transforming growth factor beta1 on bonelike tissue formation in three-dimensional cell culture. II: osteoblastic differentiation. Tissue Eng 2004; 10: 1414–1425.10.1089/1076327042500201Search in Google Scholar PubMed

[16] Liu L, Guo Y, Chen X, et al. Three-dimensional dynamic culture of pre-osteoblasts seeded in HA-CS/Col/nHAP composite scaffolds and treated with α-ZAL. Acta Biochim Biophys Sin 2012; 44: 669–677.10.1093/abbs/gms049Search in Google Scholar PubMed

[17] Liu L, Li R, Zhang L. Mechanical properties of hyaluronic acid modifying chitosan/collagen/nano-hydroxyapatite composite scaffold and its effect on osteoblast proliferation. Chin J Tissue Eng Clin Heal 2011; 15: 7127–7131.Search in Google Scholar

[18] Luginbuehl V, Wenk E, Koch A, Gander B, Merkle HP, Meinel L. Insulin-like growth factor I-releasing alginate-tricalciumphosphate composites for bone regeneration. Pharm Res 2005; 22: 940–950.10.1007/s11095-005-4589-9Search in Google Scholar PubMed

[19] Maclaine SE, Gadhari N, Pugin R, Meek RMD, Liley M, Dalby MJ. Optimizing the osteogenicity of nanotopography using block co-polymer phase separation fabrication techniques. J Orthop Res 2012; 30: 1190–1197.10.1002/jor.22076Search in Google Scholar PubMed

[20] Mi OC, Kim YJ. Fabrication of gelatin/calcium phosphate composite nanofibrous membranes by biomimetic mineralization. Int J Biol Macromol 2012; 50: 1188–1194.10.1016/j.ijbiomac.2012.04.001Search in Google Scholar PubMed

[21] Mi RJ, Shim IK, Chung HJ, et al. Local BMP-7 release from a PLGA scaffolding-matrix for the repair of osteochondral defects in rabbits. J Control Release 2012; 162: 485–491.10.1016/j.jconrel.2012.07.040Search in Google Scholar PubMed

[22] Muhit AA, Arora S, Ogawa M, et al. Peripheral quantitative CT (pQCT) using a dedicated extremity cone-beam CT scanner. Proc SPIE Int Soc Opt Eng 2013; 8672: 325–328.10.1117/12.2006939Search in Google Scholar PubMed PubMed Central

[23] Perilli E, Baruffaldi F, Visentin M, et al. MicroCT examination of human bone specimens: effects of polymethylmethacrylate embedding on structural parameters. J Microsc 2007; 225: 192–200.10.1111/j.1365-2818.2007.01731.xSearch in Google Scholar PubMed

[24] Podshivalov L, Fischer A, Bar-Yoseph PZ. On the road to personalized medicine: multiscale computational modeling of bone tissue. Arch Comput Meth Eng 2014; 21: 399–479.10.1007/s11831-014-9120-1Search in Google Scholar

[25] Salerno A, Zeppetelli S, Di Maio E, Iannace S, Netti P. Processing/structure/property relationship of multi-scaled PCL and PCL-HA composite scaffolds prepared via gas foaming and NaCl reverse templating. Biotechnol Bioeng 2011; 108: 963–976.10.1002/bit.23018Search in Google Scholar PubMed

[26] Thadavirul N, Pavasant P, Supaphol P. Improvement of dual-leached polycaprolactone porous scaffolds by incorporating with hydroxyapatite for bone tissue regeneration. J Biomater Sci Polym Ed 2014; 25: 1986–2008.10.1080/09205063.2014.966800Search in Google Scholar PubMed

Received: 2016-1-7

Accepted: 2016-8-19

Published Online: 2017-1-11

Published in Print: 2017-10-26

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Articles in the same Issue

https://doi.org/10.1515/bmt-2016-0005

Keywords for this article

bone scaffold; orthotopic transplantation; polycaprolactone; rapid prototype