Automated bioreactor system for cartilage tissue engineering of human primary nasal septal chondrocytes
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Sascha Princz
, Ulla Wenzel
Abstract
An automated bioreactor system for three-dimensional (3D) cultivation of facial cartilage replacement matrices (e.g. whole human auricles) with automatised medium exchange, gas flow and temperature control was developed. The measurement of O2 saturation and pH value in the medium was performed with a non-invasive optical method. The whole system can be observed via remote monitoring worldwide. First results demonstrated that the complete system remained sterile throughout a period of 42 days. Human chondrocytes migrated into the employed cartilage replacement matrix consisting of decellularised porcine nasoseptal cartilage (pNSC). Furthermore, an improved migration and new synthesis of aggrecan was detected. A first evaluation of the system was conducted by comparison of the results from laboratory analysis with computational fluid dynamics (CFD).
Acknowledgments
Support of the “Bundesministerium für Bildung und Forschung” (Förderkennzeichen 03FH008I3) is gratefully acknowledged.
References
[1] Andretto Amodeo C. The central role of the nose in the face and the psyche: review of the nose and the psyche. Aesth Plast Surg 2007; 31: 406–410.10.1007/s00266-006-0241-2Search in Google Scholar PubMed
[2] Chang JS, Becker SS, Park SS. Nasal reconstruction: the state of the art. Curr Opin Otolaryngol Head Neck Surg 2004; 12: 336–343.10.1097/01.moo.0000134830.38177.adSearch in Google Scholar PubMed
[3] Chen HC, Hu YC. Bioreactors for tissue engineering. Biotechnol Lett 2006; 28: 1415–1423.10.1007/s10529-006-9111-xSearch in Google Scholar PubMed
[4] Cinbiz MN, Tigli RS, Beskardes IG, Gümüsderelioglu M, Colak Ü. Computational fluid dynamics modeling of momentum transport in rotating wall perfused bioreactor for cartilage tissue engineering. J Biotechnol 2010; 150: 389–395.10.1016/j.jbiotec.2010.09.950Search in Google Scholar PubMed
[5] Concaro S, Gustavson F, Gatenholm P. Bioreactors for tissue engineering of cartilage. Adv Biochem Engin/Biotechnol 2009; 112: 125–143.10.1007/978-3-540-69357-4_6Search in Google Scholar PubMed
[6] Kuehn A, Graf A, Wenzel U, Princz S, Mantz H, Hessling M. Development of a highly sensitive spectral camera for cartilage monitoring using fluorescence spectroscopy. J Sens Sens Syst 2015; 4: 1–6.10.5194/jsss-4-289-2015Search in Google Scholar
[7] Laurie SWS, Kaban LB, Mulliken JB, Murray JE. Donor-site morbidity after harvesting rib and iliac bone. Plast Reconstr Surg 1984; 73: 933–938.10.1097/00006534-198406000-00014Search in Google Scholar PubMed
[8] Martin I, Wendt D, Heberer M. The role of bioreactors in tissue engineering. Trends Biotechnol 2004; 22: 80–86.10.1016/j.tibtech.2003.12.001Search in Google Scholar PubMed
[9] Nagata S. Modification of the stages in total reconstruction of the auricle: part I. Grafting the three-dimensional costal cartilage framework for lobule-type microtia. Plast Reconstr Surg 1994; 93: 221–230.10.1097/00006534-199402000-00001Search in Google Scholar PubMed
[10] Oseni A, Crowley C, Lowdell M, Birchall M, Butler PE, Seifalian AM. Advancing nasal reconstructive surgery: the application of tissue engineering technology. J Tissue Eng Regen Med 2012; 6: 757–768.10.1002/term.487Search in Google Scholar PubMed
[11] Oseni AO, Butler PE, Seifalian AM. Optimization of chondrocyte isolation and characterization for large-scale cartilage tissue engineering. J Surg Res 2013; 181: 41–48.10.1016/j.jss.2012.05.087Search in Google Scholar PubMed
[12] Patrachari AR, Podichetty JT, Madihally SV. Application of computational fluid dynamics in tissue engineering. J Biosci Bioeng 2012; 114: 123–132.10.1016/j.jbiosc.2012.03.010Search in Google Scholar PubMed
[13] Pörtner R, Nagel-Heyer S, Goepfert Ch, Adamietz P, Meenen NM. Bioreactor design for tissue engineering. J Biosci Bioeng 2005; 100: 235–245.10.1263/jbb.100.235Search in Google Scholar PubMed
[14] Rotter N, Bucheler M, Haisch A, Wollenberg B, Lang S. Cartilage tissue engineering using resorbable scaffolds. J Tissue Eng Regen Med 2007; 1: 411–416.10.1002/term.52Search in Google Scholar PubMed
[15] Schulz RM, Bader A. Cartilage tissue engineering and bioreactor systems for the cultivation and stimulation of chondrocytes. Eur Biophys J 2007; 36: 539–568.10.1007/s00249-007-0139-1Search in Google Scholar PubMed
[16] Schwarz S, Elsaesser AF, Koerber L, et al. Processed xenogenic cartilage as innovative biomatrix for cartilage tissue engineering: effects on chondrocyte differentiation and function. J Tissue Eng Regen Med 2015; 9: E239–E251. Doi: 10.1002/term.1650.Search in Google Scholar PubMed
[17] Schwarz S, Koerber L, Elsaesser AF, et al. Decellularized cartilage matrix as a novel biomatrix for cartilage tissue-engineering applications. Tissue Eng Part A 2012; 18: 2195–2209.10.1089/ten.tea.2011.0705Search in Google Scholar PubMed
[18] Singh H, Hutmacher DW. Bioreactor studies and computational fluid dynamics. In: Kasper C, van Griensven M, Pörtner R, editors. Bioreactor systems for tissue engineering. Berlin: Springer-Verlag 2009: 231–249.10.1007/10_2008_6Search in Google Scholar
[19] Tanzer RC. Total Reconstruction of the External Ear. Plast Rec Surg 1959; 23: 1–15.10.1097/00006534-195901000-00001Search in Google Scholar PubMed
[20] Wendt D, Jakob M, Martin I. Bioreactor-based engineering of osteochondral grafts: from model systems to tissue manufacturing. J Biosci Bioeng 2005; 100: 489–494.10.1263/jbb.100.489Search in Google Scholar PubMed
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Articles in the same Issue
- Frontmatter
- Editorial
- Engineering of viable implants
- Research articles
- Umbilical cord as human cell source for mitral valve tissue engineering – venous vs. arterial cells
- Individual construction of freeform-fabricated polycaprolactone scaffolds for osteogenesis
- Automated bioreactor system for cartilage tissue engineering of human primary nasal septal chondrocytes
- Effect of steroidal saponins-loaded nano-bioglass/phosphatidylserine/collagen bone substitute on bone healing
- Engineering of biodegradable magnesium alloy scaffolds to stabilize biological myocardial grafts
- Regular research articles
- Computation of spatio-temporal parameters in level walking using a single inertial system in lean and obese adolescents
- 445-nm diode laser-assisted debonding of self-ligating ceramic brackets
- A seepage outlet boundary condition in hemodynamics modeling
- The role of relative membrane capacitance and time delay in cerebellar Purkinje cells
- Validation and comparison of shank and lumbar-worn IMUs for step time estimation