Startseite Laser microstructured biodegradable scaffolds
Artikel
Lizenziert
Nicht lizenziert Erfordert eine Authentifizierung

Laser microstructured biodegradable scaffolds

  • Anastasia Koroleva , Olga Kufelt , Sabrina Schlie-Wolter , Ulf Hinze und Boris Chichkov EMAIL logo
Veröffentlicht/Copyright: 28. Mai 2013
Veröffentlichen auch Sie bei De Gruyter Brill

Abstract

The two-photon polymerization technique (2PP) uses non-linear absorption of femtosecond laser pulses to selectively polymerize photosensitive materials. 2PP has the ability to fabricate structures with a resolution from tens of micrometers down to hundreds of nanometers. Three-dimensional microstructuring by the 2PP technique provides many interesting possibilities for biomedical applications. This microstructuring technique is suitable with many biocompatible polymeric materials, such as polyethylene glycol, polylactic acid, polycaprolactone, gelatin, zirconium-based hybrids, and others. The process of fabrication does not require clean room conditions and does not use hazard chemicals or high temperatures. The most beneficial property of 2PP is that it is capable of producing especially complex three-dimensional (3-D) structures, including devices with overhangs, without using any supportive structure. The flexibility in controlling geometries and feature sizes and the possibility to fabricate structures without the addition of new material layers makes this technique particularly appealing for fabrication of 3-D scaffolds for tissue engineering.


Corresponding author: Boris Chichkov, Laser Zentrum Hannover, Nanotechnology, Hollerithallee 8, Hannover 30419, Germany, E-mail:

This work was supported by the DFG excellence cluster Rebirth, “From Regenerative Biology to Reconstructive Therapy.” The authors thank A. Deiwick (LZH) for performing the stem cell studies.

References

[1] Anderson JM, Shive MS. Biodegradation and biocompatibility of PLA and PLGA microspheres. Adv Drug Deliv Rev 1997; 28: 5–24.10.1016/S0169-409X(97)00048-3Suche in Google Scholar

[2] Burdick J, Chung C, Jia X, Randolph MA, Langer R. Controlled degradation and mechanical behavior of photopolymerized hyaluronic acid networks. Biomacromolecules 2005; 6: 386–391.10.1021/bm049508aSuche in Google Scholar PubMed PubMed Central

[3] Claeyssens F, Hasan EA, Gaidukeviciute A, et al. Three-dimensional biodegradable structures fabricated by two-photon polymerization. Langmuir 2009; 25: 3219–3223.10.1021/la803803mSuche in Google Scholar PubMed

[4] Day RM, Mascarenhas MM. Signal transduction associated with hyaluronan. In: Garg HG, Hales CA, editors. Chemistry and biology of hyaluronan. Amsterdam: Elsevier Ltd. 2004.10.1016/B978-008044382-9/50038-8Suche in Google Scholar

[5] Dhandayuthapani B, Yoshida Y, Maekawa T, Sakthi Kumar D. Polymeric scaffolds in tissue engineering application: a review. Int J Polym Sci 2011. Article ID 290602, 19 pages, doi:10.1155/2011/290602.10.1155/2011/290602Suche in Google Scholar

[6] Doraiswamy A, Jin C, Narayan RJ, et al. Two photon induced polymerization of organic-inorganic hybrid biomaterials for microstructured medical devices. Acta Biomater 2006; 2: 267–275.10.1016/j.actbio.2006.01.004Suche in Google Scholar PubMed

[7] El-Sayed MEH, Hoffman AS, Stayton PS. Smart polymeric carriers for enhanced intracellular delivery of therapeutic macromolecules. Expert Opin Biol Ther 2005; 5: 23–32.10.1517/14712598.5.1.23Suche in Google Scholar PubMed

[8] Gauvin R, Parenteau-Bareil R, Dokmeci MR, Merryman WD, Khademhosseini A. Hydrogels and microtechnologies for engineering the cellular microenvironment. Wiley Interdiscip Rev Nanomed Nanobiotechnol 2012; 4: 235–246.10.1002/wnan.171Suche in Google Scholar PubMed

[9] Gill AA, Claeyssens F. 3D structuring of biocompatible and biodegradable polymers via stereolithography. 3D Cell Cult 2011; 695: 309–321.10.1007/978-1-60761-984-0_19Suche in Google Scholar PubMed

[10] Gittard SD, Koroleva A, Nguyen A, et al. Two-photon polymerization microstructuring in regenerative medicine. Front Biosci Elite 2013; 5: 602–609.10.2741/E642Suche in Google Scholar PubMed

[11] Gittard SD, Miller PR, Boehm RD, et al. Multiphoton microscopy of transdermal quantum dot delivery using two photon polymerization-fabricated polymer microneedles. Faraday Discuss 2011; 149: 171–185, discussion 227–245.10.1039/C005374KSuche in Google Scholar PubMed PubMed Central

[12] Gittard SD, Narayan RJ. Laser direct writing of micro- and nano-scale medical devices. Expert Rev Med Devices 2010; 7: 343–356.10.1586/erd.10.14Suche in Google Scholar PubMed PubMed Central

[13] Gittard SD, Ovsianikov A, Chichkov BN, Doraiswamy A, Narayan RJ. Two-photon polymerization of microneedles for transdermal drug delivery. Expert Opin Drug Deliv 2010; 7: 513–533.10.1517/17425241003628171Suche in Google Scholar PubMed PubMed Central

[14] Gurav N, Lutolf MP, Raeber GP, Hubbell JA, Di Silvio L. Differentiation of human bone marrow stem cells within RGD functionalised, proteolytically sensitive PEG gels. Tissue Eng 2007; 13: 1675–1675.Suche in Google Scholar

[15] Harley BAC, Kim H-D, Zaman MH, Yannas IV, Lauffenburger DA, Gibson LJ. Microarchitecture of three-dimensional scaffolds influences cell migration behavior via junction interactions. Biophys J 2008; 95: 4013–4024.10.1529/biophysj.107.122598Suche in Google Scholar PubMed PubMed Central

[16] Hoffman AS, Stayton PS. Conjugates of stimuli-responsive polymers and proteins. Prog Polym Sci 2007; 32: 922–932.10.1016/j.progpolymsci.2007.05.005Suche in Google Scholar

[17] Ifkovits JL, Burdick JA. Photopolymerizable and degradable biomaterials for tissue engineering applications. Tissue Eng 2007; 13: 2369–2385.10.1089/ten.2007.0093Suche in Google Scholar PubMed

[18] Jia C, Khademhosseini A, Dehghania F. Enhancing cell penetration and proliferation in chitosan hydrogels for tissue engineering applications. Biomaterials 2011; 32: 9719–9729.10.1016/j.biomaterials.2011.09.003Suche in Google Scholar PubMed

[19] Juodkazis S, Mizeikis V, Seet KK, Miwa M, Misawa H. Two-photon lithography of nanorods in SU-8 photoresist. Nanotechnology 2005; 16: 846–849.10.1088/0957-4484/16/6/039Suche in Google Scholar

[20] Knudson CB, Knudson W. Hyaluronan-binding proteins in development, tissue homeostasis, and disease. FASEB J 1993; 107: 1233–1241.Suche in Google Scholar

[21] Koroleva A, Gill AA, Ortega I, et al. Two-photon polymerization-generated and micromolding-replicated 3D scaffolds for peripheral neural tissue engineering applications. Biofabrication 2012; 4: 025005.10.1088/1758-5082/4/2/025005Suche in Google Scholar PubMed

[22] Koroleva A, Gittard SD, Schlie S, Deiwick A, Jockenhoevel S, Chichkov BN. Fabrication of fibrin scaffolds with controlled microscale architecture by a two-photon polymerization – micromolding technique. Biofabrication 2012; 4: 015001.10.1088/1758-5082/4/1/015001Suche in Google Scholar PubMed

[23] Lepperdinger G, Fehrer C, Reitinger S. Biodegradation of hyaluronan. In: Garg HG, Hales CA, editors. Chemistry and biology of hyaluronan. Amsterdam: Elsevier Ltd., 2004.Suche in Google Scholar

[24] Ma PX. Scaffolds for tissue fabrication. Mater Today 2004; 7: 30–40.10.1016/S1369-7021(04)00233-0Suche in Google Scholar

[25] Melissinaki V, Gill AA, Ortega I, et al. Direct laser writing of 3D scaffolds for neural tissue engineering applications. Biofabrication 2011; 3: 045005.10.1088/1758-5082/3/4/045005Suche in Google Scholar

[26] Nguyen AK, Gittard SD, Koroleva A, et al. Two-photon polymerization of polyethylene glycol diacrylate scaffolds with riboflavin and triethanolamine used as a water-soluble photoinitiator. Future Medicine. In press.Suche in Google Scholar

[27] Nicodemus GD, Bryant SJ. Cell encapsulation in biodegradable hydrogels for tissue engineering applications. Tissue Eng Part B-Rev 2008; 14: 149–165.10.1089/ten.teb.2007.0332Suche in Google Scholar

[28] Ovsianikov A, Chichkov B, Mente P, Monteiro-Riviere NA, Doraiswamy A, Narayan RJ. Two photon polymerization of polymer-ceramic hybrid materials for transdermal drug delivery. Int J Appl Ceram Technol 2007; 4: 22–29.10.1111/j.1744-7402.2007.02115.xSuche in Google Scholar

[29] Ovsianikov A, Deiwick A, Van Vlierberghe S, et al. Laser fabrication of 3D gelatin scaffolds for the generation of bioartificial tissues. Materials 2011; 4: 288–299.10.3390/ma4010288Suche in Google Scholar

[30] Ovsianikov A, Malinauskas M, Schlie S, et al. Three-dimensional laser micro- and nanostructuring of polyethylene glycol materials and evaluation of their cytotoxicity for tissue engineering applications. Acta Biomater 2011; 7: 967–974.10.1016/j.actbio.2010.10.023Suche in Google Scholar

[31] Ovsianikov O, Mironov V, Stampfl J, Liska R. Engineering 3D cell-culture matrices: multiphoton processing technologies for biological and tissue engineering applications. Exp Rev Med Dev 2012; 9: 613–633.10.1586/erd.12.48Suche in Google Scholar

[32] Peppas NA, Kim B. Stimuli-sensitive protein delivery systems. J Drug Deliv Sci Technol 2006; 16: 11–18.10.1016/S1773-2247(06)50002-4Suche in Google Scholar

[33] Raimondi MT, Eaton SM, Nava MM, Lagana M, Cerullo G, Osellame R. Two-photon laser polymerization: from fundamentals to biomedical application in tissue engineering and regenerative medicine. J Appl Biomater Biomech 2012; 10: 55–65.10.5301/JABFM.2012.9278Suche in Google Scholar PubMed

[34] Schlie S. Selective cell control for biomedical applications – impact of laser-fabricated 3D scaffolds and surface topographies. SVH 2010; ISBN 978-3-8381-1814-7.Suche in Google Scholar

[35] Schlie S, Ngezahayo A, Ovsianikov A, et al. Three-dimensional cell growth on structures fabricated from Ormocer® by two-photon polymerisation technique. J Biomater App 2007; 22: 275–287.10.1177/0885328207077590Suche in Google Scholar PubMed

[36] Smeds KA, Grinstaff MW. Photocrosslinkable polysaccharides for in situ hydrogel formation. J Biomed Mater Res 2001; 54: 115–121.10.1002/1097-4636(200101)54:1<115::AID-JBM14>3.0.CO;2-QSuche in Google Scholar

[37] Turley EA, Noble PW, Bourguignon LYW. Signaling properties of hyaluronan receptors. J Biol Chem 2002; 277: 4589–4592.10.1074/jbc.R100038200Suche in Google Scholar

[38] Weber LM, Hayda KN, Haskins K, Anseth KS. The effects of cell-matrix interactions on encapsulated β-cell function within hydrogels functionalized with matrix-derived adhesive peptides. Biomaterials 2007; 28: 3004–3011.10.1016/j.biomaterials.2007.03.005Suche in Google Scholar

Received: 2012-12-12
Accepted: 2013-4-29
Published Online: 2013-05-28
Published in Print: 2013-10-01

©2013 by Walter de Gruyter Berlin Boston

Heruntergeladen am 30.11.2025 von https://www.degruyterbrill.com/document/doi/10.1515/bmt-2013-0036/html
Button zum nach oben scrollen