Identification and expression analyses of two lotus (Nelumbo nucifera) dehydrin genes in response to adverse temperatures, ABA and IAA treatments
-
Chen Dong
,
Meigui Yang
Abstract
Dehydrins (DHNs) play imperative role in the stress tolerance of high plants. In this study, the YSK2-type DHN (NnDHNl) and SK3-type DHN (NnDHN2) were investigated. The putative NnDHNl and NnDNH2 shared conserved S-segment as well as K-segment, with distinct isoelectric points, kinase selectivities and number of functional motifs. Phylogenetic analysis revealed that NnDHNs were grouped with YnSKn- and SKn-type of DHNs in high plants. Additionally,NnDHN1 mRNA was only expressed in leaves and embryo. NnDHN2 mRNA was found in embryo, leaves, rhizome and leafstalk.NnDHNs mRNAs were not detected in root of N. nucifera. Real-time PCR assay demonstrated that the levels of NnDHN1 and NnDHN2 mRNAs were significantly elevated during cold temperature, while both were relatively stable in response to high temperature. Moreover, NnDHN2 mRNA was significantly increased after treatment with abscisic acid (ABA) or indole-3-acetic acid (IAA), while NnDHN1 mRNA was induced by ABA treatment with little change in response to IAA. These results illustrate two NnDHNs with partially overlapping function which exhibit different stress responsiveness.
Acknowledgements
The authors are greatly appreciative of the reviewers for their helpful comments and detailed suggestions. This work is financially supported by National Natural Science Foundation of China (no. 31601366), Foundation of Henan Educational Committee (no. 16A180024), Doctoral Scientific Research Start-up Foundation from Henan University of Technology (no. 31400855) and Fundamental Research Funds for the Henan Provincial Colleges and Universities in Henan University of Technology (no. 2015QNJH07).
References
Alsheikh M.K., Svensson J. & Randall S.K. 2005. Phosphorylation regulated ion-binding is a property shared by the acidic subclass dehydrins. Plant Cell Environ. 28:1114–1122.10.1111/j.1365-3040.2005.01348.xSuche in Google Scholar
Babic M., Radic S., Cvjetko P., Roje V., Pevalek-Kozlina B. & Pavlica M. 2009. Antioxidative response of Lemna minor plants exposed to thallium (I)-acetate. Aquatic Bot. 91:166–172.10.1016/j.aquabot.2009.05.005Suche in Google Scholar
Bies-Etheve N., Gaubier-Comella P., Debures A., Lasserre E., Jobet E., Raynal M., Cooke R. & Delseny M. 2008. Inventory, evolution and expression profiling diversity of the LEA (late embryogenesis abundant) protein gene family in Arabidopsis thaliana. Plant. Mol. Biol.67:107–124.10.1007/s11103-008-9304-xSuche in Google Scholar PubMed
Brini F., Yamamoto A., Jlaiel L., Takeda S., Hobo T., Dinh H.Q., Hattori T., Masmoudi K. & Hanin M. 2011. Pleiotropic effects of the wheat dehydrin DHN-5 on stress responses in Arabidopsis. Plant Cell Physiol.52: 676–688.10.1093/pcp/pcr030Suche in Google Scholar PubMed
Dong C., Zheng X., Diao Y., Wang Y., Zhou M. & Hu Z. 2015. Molecular cloning and expression analysis of a catalase gene (NnCAT) from Nelumbo nucifera. Appl. Biochem. Biotechnol. 177: 1216–1228.10.1007/s12010-015-1808-7Suche in Google Scholar PubMed
Eriksson S.K., Kutzer M., Procek J., Grobner G. & Harryson P. 2011. Tunable membrane binding of the intrinsically disordered dehydrin Lti30, a cold-induced plant stress protein. Plant Cell 23: 2391–2404.10.1105/tpc.111.085183Suche in Google Scholar PubMed PubMed Central
Hara M., Shinoda Y., Tanaka Y. & Kuboi T. 2009. DNA binding of citrus dehydrin promoted by zinc ion. Plant Cell Environ. 32: 532–541.10.1111/j.1365-3040.2009.01947.xSuche in Google Scholar PubMed
Hughes S. & Graether S.P. 2011. Cryoprotective mechanism of a small intrinsically disordered dehydrin protein. Protein Sci. 20: 42–50.10.1002/pro.534Suche in Google Scholar PubMed PubMed Central
Hundertmark M. & Hincha D.K. 2008. LEA (Late Embryogenesis Abundant) proteins and their encoding genes in Arabidopsis thaliana. BMC Genomics 9:118.10.1186/1471-2164-9-118Suche in Google Scholar PubMed PubMed Central
Hundertmark M., Buitink J., Leprince O. & Hincha D.K. 2011. Reduction of seed-specific dehydrins reduces seed longevity in Arabidopsis thaliana. Seed Sci. Res. 21:165–173.10.1017/S0960258511000079Suche in Google Scholar
Kim S.Y. & Nam K.H. 2010. Physiological roles of ERD10 in abiotic stresses and seed germination of Arabidopsis. Plant Cell Rep. 29: 203–209.10.1007/s00299-009-0813-0Suche in Google Scholar PubMed
Koag M.C., Wilkens S., Fenton R.D., Resnik J., Vo E. & Close TJ. 2009. The K-segment of maize DHN1 mediates binding to anionic phospholipid vesicles and concomitant structural changes. Plant. Physiol. 150: 1503–1514.10.1104/pp.109.136697Suche in Google Scholar PubMed PubMed Central
Lescot M., Dehais P., Thijs G., Marchal K., Moreau Y., Van de peer Y., Rouze P. & Rombauts S. 2002. PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Res. 30: 325–327.10.1093/nar/30.1.325Suche in Google Scholar PubMed PubMed Central
Livak K.J. & Schmittgen T.D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔ CT method. Methods (San Diego, Calif.). 25: 402–408.10.1006/meth.2001.1262Suche in Google Scholar PubMed
Peng Y., Reyes J.L., Wei H., Yang Y., Karlson D., Covarrubias A.A., Krebs S.L., Fessehaie A. & Arora R. 2008. RcDhn5, a cold acclimation-responsive dehydrin from Rhododendron catawbiense rescues enzyme activity from dehydration effects in vitro and enhances freezing tolerance in RcDhn5-overexpressing Arabidopsis plants. Physiol. Plant. 134: 583–597.10.1111/j.1399-3054.2008.01164.xSuche in Google Scholar PubMed
Priestley D.A. & Posthumus M.A. 1982. Extreme longevity of lotus seeds from Pulantien. Nature 299: 148–149.10.1038/299148a0Suche in Google Scholar
Riera M., Figueras M., Lopez C., Goday A. & Pages M. 2004. Protein kinase CK2 modulates developmental functions of the abscisic acid responsive protein Rab17 from maize. Proc. Natl. Acad. Sci. USA 101:9879–9884.10.1073/pnas.0306154101Suche in Google Scholar PubMed PubMed Central
Shekhawat U.K., Srinivas L & Ganapathi T.R. 2011. MusaDHN1, a novel multiple stress-inducible SK3-type dehydrin gene, contributes affirmatively to drought- and salt-stress tolerance in banana. Planta 234: 915.10.1007/s00425-011-1455-3Suche in Google Scholar PubMed
Shen-Miller J., Lindner P., Xie Y., Villa S., Wooding K., Clarke S.G., Loo R.R. & Loo J.A. 2013. Thermal-stable proteins of fruit of long-living sacred Lotus Nelumbo nucifera Gaertn var. China Antique. Tropical. Plant. Biol.6: 69–84.10.1007/s12042-013-9124-2Suche in Google Scholar PubMed PubMed Central
Sridhar K.R. & Bhat R. 2007. Lotus-A potential nutraceutical source. J. Agric. Technol. 3: 143–145.Suche in Google Scholar
Tamura K., Dudley J., Nei M. & Kumar S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24: 1596–1599.10.1093/molbev/msm092Suche in Google Scholar PubMed
Vaseva I.I., Anders I., Yuperlieva-Mateeva B., Nenkova R., Kostadinova A. & Feller U. 2014a. Dehydrin expression as a potential diagnostic tool for cold stress in white clover. Plant. Physiol. Biochem. 78: 43–48.10.1016/j.plaphy.2014.02.014Suche in Google Scholar PubMed
Vaseva I.I., Anders I. & Feller U. 2014b. Identification and expression of different dehydrin subclasses involved in drought response of Trifolium repens. J. Plant. Physiol. 171: 213–224.10.1016/j.jplph.2013.07.013Suche in Google Scholar PubMed
Wang X., Zhu H., Jin G., Liu H., Wu W. & Zhu J. 2007. Genomescale identification and analysis of LEA genes in rice (Oryza sativa L.). Plant. Sci. 172: 414–420.10.1016/j.plantsci.2006.10.004Suche in Google Scholar
Xu J., Zhang Y., Guan Z., Wei W., Han L. & Chai T. 2008. Expression and function of two dehydrins under environmental stresses in Brassica juncea L. Mol. Breeding 21: 431–438.10.1007/s11032-007-9143-5Suche in Google Scholar
Xu Y. & Crouch J. 2008. Marker-assisted selection in plant breeding: from publi-cations to practice. Crop Sci. 48: 391–407.10.2135/cropsci2007.04.0191Suche in Google Scholar
Yamasaki Y., Koehler G., Blacklock B.J. & Randall S.K. 2013. Dehydrin expression in soybean. Plant Physiol. Biochem. 70: 213–220.10.1016/j.plaphy.2013.05.013Suche in Google Scholar PubMed
Yang Y., He M., Zhu Z., Li S., Xu Y., Zhang C., Singer S.D. & Wang Y. 2012. Identification of the dehydrin gene family from grapevine species and analysis of their responsiveness to various forms of abiotic and biotic stress. BMC Plant Biol. 12: 140.10.1186/1471-2229-12-140Suche in Google Scholar PubMed PubMed Central
© 2017 Institute of botany, Slovak Academy of Sciences
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- Botany
- Comparative morphoanatomical analysis of the leaves and stems of Daphne (Thymelaeaceae) species
- Botany
- Effect of the Gabčíkovo Waterworks (Slovakia) on riparian floodplain forest ecosystems in the Danube inland delta: vegetation dynamics and trends
- Botany
- Effects of different light qualities on seedling growth and chlorophyll fluorescence parameters of Dendrobium officinale
- Botany
- Identification and expression analyses of two lotus (Nelumbo nucifera) dehydrin genes in response to adverse temperatures, ABA and IAA treatments
- Botany
- Characterization and expression analysis of a chalcone isomerase-like gene in relation to petal color of Actinidia chrysantha
- Cellular and Molecular Biology
- Citrus flavonoids collectively dominate the α-amylase and α-glucosidase inhibitions
- Zoology
- Distribution, diversity and some ecological characteristics of ostracods (Crustacea: Ostracoda) in Gökçeada (Imbros) Island (Northern Aegean Sea, Turkey)
- Zoology
- Native crab and crayfish co-occurrence: First evidence in Europe
- Zoology
- DNA barcoding and first records of two rare Adicella species (Trichoptera: Leptoceridae) in Croatia
- Zoology
- Occurrence of Tomicobia seitneri (Hymenoptera: Pteromalidae) and Ropalophorus clavicornis (Hymenoptera: Braconidae) in Ips typographus adults (Coleoptera: Curculionidae: Scolytinae) from Austria, Poland and France
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