Yield improvement of the king oyster mushroom, Pleurotus eryngii, by transformation of its cellulase gene
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Urarux Romruen
Abstract
A plasmid pCAMBIA1301 containing Pleurotus eryngii cellulase gene (PEcbh), under the control of Lentinus edodes glyceraldehyde-3-phosphate dehydrogenase (LEgpd) promoter, was constructed and used as an expression vector. The vector was transformed into the tissue of P. eryngii using Agrobacterium tumefaciens-mediated transformation (ATMT) method and 4 transformants (PET1-4) were obtained. The positive transformants were confirmed by cultivation in media containing hygromycin and by PCR amplification of hygromycin B resistance-LEgpd promoter gene fragment. Unpredicted, cellulase specific activities of the transformants were not higher than those of the wild type. Mushroom cultivation was performed in the laboratory and the results revealed that the average biological efficiency of PET4 was significantly 1.52 times higher than those of the wild type.
Acknowledgements
This work was financially supported by Silpakorn University Research and Development Institute (Grant No. SURDI 54/01/10). U.R. was granted by Science Achievement Scholarship of Thailand from The Thailand Research Fund as a Ph.D. student.
- ATMT
Agrobacterium tumefaciens-mediated transformation
- BE
biological efficiency
- CMC
carboxymethylcellulose
- gus
β-glucuronidase
- hph
hygromycin B resistance
- LEgpd
Lentinus edodes glyceraldehyde-3-phosphate dehydrogenase
- PDA
potato dextrose agar
- PEcbh
Pleurotus eryngii cellulase gene
- PET1-4
Pleurotus eryngii transformant strains 1-4
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© 2017 Institute of Molecular Biology, Slovak Academy of Sciences
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- Botany
- Allelopathy: An overview from micro- to macroscopic organisms, from cells to environments, and the perspectives in a climate-changing world
- Cellular and Molecular Biology
- Prospecting soil bacteria from subtropical Brazil for hydrolases production
- Cellular and Molecular Biology
- Yield improvement of the king oyster mushroom, Pleurotus eryngii, by transformation of its cellulase gene
- Botany
- Accumulation and physiological response of cadmium in Hydrocharis dubia
- Botany
- Development of an efficient Agrobacterium mediated transformation system for chickpea (Cicer arietinum)
- Botany
- Distribution and ecology of high-mountain Solidago minuta (Asteraceae) in Poland
- Zoology
- Ecology of moss-dwelling rotifers in a raised bog: Differentiation of rotifer communities in microhabitats
- Zoology
- Harvestmen (Opiliones) communities in an arboretum: Influence of tree species
- Zoology
- A crossover design to assess feeding preferences in terrestrial isopods: A case study in a Mediterranean species
- Zoology
- Ecology-based mapping of mosquito breeding sites for area-minimized BTI treatments
- Zoology
- Does the invasion of Northern Red Oak Quercus rubra in parkland influence the diversity of birds?
- Zoology
- Prevalence of dorsal notch and variations in the foramen magnum shape in dogs of different breeds and morphotypes
- Cellular and Molecular Biology
- Low density lipoprotein increases amyloid precursor protein processing to amyloidogenic pathway in differentiated SH-SY5Y cells
