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Prospecting soil bacteria from subtropical Brazil for hydrolases production

  • Caroline T. De Oliveira , Jamile Q. Pereira , Adriano Brandelli and Daniel J. Daroit EMAIL logo
Published/Copyright: February 28, 2017
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Biologia
From the journal Biologia Volume 72 Issue 2

Abstract

Eighteen bacterial strains were isolated from soil of an urban area located in a transition zone between the Atlantic Forest and Pampa biomes, in southern Brazil. These strains were screened for cellulolytic, lipolytic and proteolytic potentials. Eleven isolates (61%) were able to produce cellulolytic enzymes on carboxymethylcellulose (CMC) agar plates, 14 isolates (78%) were proteolytic on skim milk agar plates, and all isolates demonstrated lipolytic/esterolytic potential on tributyrin agar (TBA) plates. From the 18 bacteria, nine (50%) were shown to produce the three investigated enzyme activities. Selected isolates were then evaluated for growth and enzyme production at different conditions of temperature and pH on CMC agar, TBA, and feather meal agar plates. As a general trend, growth and hydrolysis zones were observed at pH 6.0–9.0 and 30–37∘C. Sequencing of 16S rRNA gene fragments indicated that 10 isolates belonged to the genus Bacillus, three to Lysinibacillus genus, and the remaining isolates were representatives of Serratia, Phyllobacterium, Paenibacillus, Acinetobacter, and Curtobacterium. The isolate Bacillus sp. CL18 displayed competence for feather degradation when cultured in mineral medium (30∘C, pH 7.0) containing a single feather as the only organic substrate. Results from bioprospection indicate the functional versatility of the bacterial isolates, which might be of significance from both ecological and biotechnological perspectives.

Key words: culturable bacteria; bioprospecting; cellulase; lipolytic potential; protease

*Electronic supplementary material. The online version of this article (DOI: 10.1515/biolog-2017-0025) contains supplementary material, which is available to authorized users.

Acknowledgements

C.T. Oliveira and D.J. Daroit thank Programa Institucional de Iniciação Científica e Tecnológica (PRO-ICT/UFFS), and Programa Institucional de Bolsas de Iniciaçăo Científica (PROBIC/FAPERGS/UFFS).

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Abbreviations
CMC

carboxymethylcellulose

CTAB

cetyltrimethylammonium bromide

EDTA

ethylenediaminetetraacetic acid

FMA

feather meal agar

MM

mineral medium

PCA

plate count agar

SMA

skim milk agar

TBA

tributyrin agar

UHT

ultra high temperature

Received: 2017-1-6
Accepted: 2017-2-25
Published Online: 2017-2-28
Published in Print: 2017-2-1

© 2017 Institute of Molecular Biology, Slovak Academy of Sciences

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https://doi.org/10.1515/biolog-2017-0025
Keywords for this article
culturable bacteria; bioprospecting; cellulase; lipolytic potential; protease

Articles in the same Issue

  1. Botany
  2. Allelopathy: An overview from micro- to macroscopic organisms, from cells to environments, and the perspectives in a climate-changing world
  3. Cellular and Molecular Biology
  4. Prospecting soil bacteria from subtropical Brazil for hydrolases production
  5. Cellular and Molecular Biology
  6. Yield improvement of the king oyster mushroom, Pleurotus eryngii, by transformation of its cellulase gene
  7. Botany
  8. Accumulation and physiological response of cadmium in Hydrocharis dubia
  9. Botany
  10. Development of an efficient Agrobacterium mediated transformation system for chickpea (Cicer arietinum)
  11. Botany
  12. Distribution and ecology of high-mountain Solidago minuta (Asteraceae) in Poland
  13. Zoology
  14. Ecology of moss-dwelling rotifers in a raised bog: Differentiation of rotifer communities in microhabitats
  15. Zoology
  16. Harvestmen (Opiliones) communities in an arboretum: Influence of tree species
  17. Zoology
  18. A crossover design to assess feeding preferences in terrestrial isopods: A case study in a Mediterranean species
  19. Zoology
  20. Ecology-based mapping of mosquito breeding sites for area-minimized BTI treatments
  21. Zoology
  22. Does the invasion of Northern Red Oak Quercus rubra in parkland influence the diversity of birds?
  23. Zoology
  24. Prevalence of dorsal notch and variations in the foramen magnum shape in dogs of different breeds and morphotypes
  25. Cellular and Molecular Biology
  26. Low density lipoprotein increases amyloid precursor protein processing to amyloidogenic pathway in differentiated SH-SY5Y cells
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