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Isolation and characterization of Vibrio alginolyticus from cultured amphioxus Branchiostoma belcheri tsingtauense

  • Yuxia Zou , Cuiping Ma , Yanfei Zhang , Zongjun Du , Feng You , Xungang Tan EMAIL logo and Pei-Jun Zhang
Published/Copyright: August 11, 2016
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Biologia
From the journal Biologia Volume 71 Issue 7

Abstract

Amphioxus is an intermediary species bridging invertebrates and vertebrates used to study the development and evolution of species and genes. In this study, the dominant bacterial strain was isolated from tail lesions of cultured amphioxus and designated WW1. Challenge by intramuscular microinjection revealed the 50% lethal dose of WW1 to be 9 × 102 bacteria/g amphioxus body weight. Based on 16S rRNA gene sequence, physiological and biochemical characteristics, and species-specific collagenase gene marker, WW1 was identified as Vibrio alginolyticus. To our knowledge, this is the first report on the isolation of V. alginolyticus as pathogen from amphioxus. This study extends the known host range of V. alginolyticus and may provide information helpful in preventing and managing its occurrence in cultured animals.

Key words: amphioxus; 16S rRNA gene; collagenase; pathogen isolation; experimental challenge; Vibrio alginolyticus

Acknowledgements

This work was supported by The National Sciences Foundation of China (No. 30972270), the National High-Technology Research and Development Program (863 Program) of China (2008AA092600), and the Scientific and Technological Innovation Project financially supported by Qingdao National Laboratory for Marine Science and Technology (No. 2015ASKJ02).

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Abbreviations
NS

normal saline

TCBS

thiosulphate citrate bile salts sucrose

Received: 2016-12-12
Accepted: 2016-6-15
Published Online: 2016-8-11
Published in Print: 2016-7-1

©2016 Institute of Molecular Biology, Slovak Academy of Sciences

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https://doi.org/10.1515/biolog-2016-0102
Keywords for this article
amphioxus; 16S rRNA gene; collagenase; pathogen isolation; experimental challenge; Vibrio alginolyticus

Articles in the same Issue

  1. Zoology
  2. Climate warming and invasive fish species: Will they replace native fish species in waters of temperate zones?
  3. Cellular and Molecular Biology
  4. Production of poly-3-hydroxybutyrate from mixed culture
  5. Cellular and Molecular Biology
  6. Molecular and immunological characterization of Lp1, the 34 kDa outer membrane lipoprotein of Pseudomonas aeruginosa
  7. Cellular and Molecular Biology
  8. Antibacterial activity of a microemulsion loaded with cephalosporin
  9. Cellular and Molecular Biology
  10. Isolation and characterization of Vibrio alginolyticus from cultured amphioxus Branchiostoma belcheri tsingtauense
  11. Cellular and Molecular Biology
  12. Somatic embryogenesis in Phlox paniculata – histological analysis
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