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Production of poly-3-hydroxybutyrate from mixed culture

  • Thomas Shalin , Raveendran Sindhu EMAIL logo , Ashok Pandey , Vincenza Faraco und Parameswaran Binod
Veröffentlicht/Copyright: 11. August 2016
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Biologia
Aus der Zeitschrift Biologia Band 71 Heft 7

Abstract

Poly-3-hydroxybutyrate (PHB) is a biodegradable polymer produced by many bacteria. Some of the properties are similar to thermoplastics like polypropylene, hence finding application PHB can directly replace non-biodegradable polymers. But the main barrier has been the cost difference. The utilization of mixed microbial cultures facilitates the use of complex substrates and thus can reduce the cost of PHB production. In the present study, mixed culture systems, where metabolite produced by one organism may be assimilated by the other organism, were employed. Bacillus firmus NII 0830, the first organism, was used for the production of PHB since it accumulates a large amount of PHB, while the second organism, Lactobacillus delbrueckii NII 0925, was used to provide acetic acid. Enzyme kinetic studies were performed on the PHB biosynthetic enzymes, such as β-ketothiolase, acetoacetyl CoA reductase and PHB synthase. PHB production by mixed culture was higher when compared to pure cultures. The mathematical model was then fitted to the experimental data, which can describe the dynamics of a mixed culture. The β-ketothiolase and acetoacetyl CoA reductase showed a Vmax value of 0.0093 μM/min and 0.0253 μM/min, respectively. The Km values were 140.8 μM and 183.5 μM, respectively. The enzyme kinetic studies gave an idea about the action of the enzymes.

Key words: poly-3-hydroxybutyrate; fermentation; mixed culture; biopolymer; production
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Acknowledgements

Authors are grateful to the Department of Biotechnology, Government of India, New Delhi, for financial support of the project. One of the authors, Raveendran Sindhu, acknowledges the Department of Biotechnology for financial support under DBT Bio-CARe scheme. Raveendran Sindhu and Parameswaran Binod acknowledge European Commission Seventh Framework Programme, Marie Curie Actions-International Research Staff Exchange Scheme - Contact Number 318931.

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Abbreviations
DTNB

dithionitrobenzoic acid

MCB

mixed culture biotechnology

PHB

poly-3-hydroxybutyrate

Received: 2016-1-11
Accepted: 2016-7-7
Published Online: 2016-8-11
Published in Print: 2016-7-1

©2016 Institute of Molecular Biology, Slovak Academy of Sciences

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https://doi.org/10.1515/biolog-2016-0097
Schlagwörter für diesen Artikel
poly-3-hydroxybutyrate; fermentation; mixed culture; biopolymer; production

Artikel in diesem Heft

  1. Zoology
  2. Climate warming and invasive fish species: Will they replace native fish species in waters of temperate zones?
  3. Cellular and Molecular Biology
  4. Production of poly-3-hydroxybutyrate from mixed culture
  5. Cellular and Molecular Biology
  6. Molecular and immunological characterization of Lp1, the 34 kDa outer membrane lipoprotein of Pseudomonas aeruginosa
  7. Cellular and Molecular Biology
  8. Antibacterial activity of a microemulsion loaded with cephalosporin
  9. Cellular and Molecular Biology
  10. Isolation and characterization of Vibrio alginolyticus from cultured amphioxus Branchiostoma belcheri tsingtauense
  11. Cellular and Molecular Biology
  12. Somatic embryogenesis in Phlox paniculata – histological analysis
  13. Botany
  14. Overexpression of the novel Zygophyllum xanthoxylum C2H2-type zinc finger gene ZxZF improves drought tolerance in transgenic Arabidopsis and poplar
  15. Botany
  16. Vegetation diversity of the two Dinaric karstic rivers in Bosnia and Herzegovina
  17. Botany
  18. Diversity and spatial patterns of plant communities in volcanic temporary ponds of Sicily (Italy)
  19. Zoology
  20. Erythraeus (Erythraeus) pistacicus sp. n. (Trombidiformes: Erythraeidae) from southern Iran, and notes on other Erythraeus spp.
  21. Zoology
  22. Studies on eucrenal-hypocrenal zonation of springs along the river mainstream: A case study of a karst canyon in Bosnia and Herzegovina
  23. Zoology
  24. Different catching series from light and pheromone trapping of Helicoverpa armigera (Lepidoptera: Noctuidae)
  25. Zoology
  26. Newts in the nature reserve Dönche: Evaluation of diversity, breeding behaviour and threats due to a decline in habitat suitability
  27. Zoology
  28. Antioxidant status of rabbits after treatment with epicatechin and patulin
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