Effects of heat stress on serum cortisol, alkaline phosphatase activity and heat shock protein 40 and 90β mRNA expression in rainbow trout Oncorhynchus mykiss
-
Zhen Li
, Yan Ni Wang
Abstract
The enhanced expression of heat shock proteins (Hsps) in organisms is associated with environmental temperatures. For rainbow trout (Oncorhynchus mykiss), high temperature is a major abiotic stress limiting its growth and productivity. In the present study, we have investigated serum cortisol (COR) and alkaline phosphatase (AKP) activity as well as Hsp40 and Hsp90β mRNA expression in diflerent tissues (gill, liver, spleen, heart, brain and head kidney) of rainbow trout under unstressed (18°C) and heat-stressed (25°C) conditions. Consequently, COR at 8 H, and heat stress recovering (HSR) AKP at 2, 8 and 12 H were significantly up-regulated in the heat stressed group (P > 0.05). Hsp40 and Hsp90β mRNA expressions were significantly up-regulated in all tissues and responded to heat stress in a tissue- and time-specific manner. Their expression in heart and brain were relatively high in the unstressed group compared with other tissues (P > 0.05). During heat stress, Hsp40 and Hsp90β in all tissues showed the first rise after falling trend, and the highest induction was recorded in the spleen (P > 0.05). Hsp40 in heart and brain, and Hsp90β in liver and brain did not return to the comparison group (0 h) after HSR. The results indicate that Hsp40 and Hsp90β are more important in dealing with heat-induced stress and mainly afforded protection in liver, heart and brain. In addition, cells may be damaged under heat stress with increasing AKP activity and elevating COR, which regulates energy metabolism and fights environmental pressure to maintain homeostasis of rainbow trout.
The paper was presented at the 13th International Symposium on Aquatic Oligochaeta, Brno, Czech Republic, 7–11 September, 2015
Abbreviations: AKP, alkaline phosphatase; COR, cortisol; HSFs, heat shock transcription factors; Hsps, heat shock proteins; HSR, heat-stress recovering; JAK-STAT, just another kinase’ signal transduction and transcription; PKR, protein kinase resource.
Acknowledgements
This research was supported by the National Natural Science Foundation of China (No. 31460687).
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© 2016 Institute of Molecular Biology, Slovak Academy of Sciences
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Artikel in diesem Heft
- Zoology
- 13th International Symposium on Aquatic Oligochaeta, Brno, Czech Republic, 7–11 September, 2015
- Zoology
- Fate of Lamprodrilus isoporus (Oligochaeta: Lumbriculidae) in eutrophic lakes
- Zoology
- Alien Naididae species (Annelida: Clitellata) and their role in aquatic habitats in Poland
- Zoology
- Groundwater oligochaetes (Annelida: Clitellata) of the Dinaric region (South-East Europe)
- Zoology
- Clitellate communities in anomalous region of Lake Baikal, Eastern Siberia (Russia)
- Zoology
- The aquatic oligochaetes (Annelida: Clitellata) of eight lakes in the Aşği Firat River Basin (Lower Euphrates, Turkey)
- Zoology
- Aquatic oligochaetes (Annelida: Clitellata) of seven lakes in the Ceyhan River basin (Turkey)
- Zoology
- The use of BMWP and ASPT indices for evaluation of water quality according to macroinvertebrates in Küçük Menderes River (Turkey)
- Zoology
- The aquatic annelid community in Lake Al-Delmage (Iraq)
- Zoology
- Preliminary survey of freshwater Oligochaeta from selected districts in Tamil Nadu (India)
- Cellular and Molecular Biology
- Bacterial communities inhabiting toxic industrial wastewater generated during nitrocellulose production
- Cellular and Molecular Biology
- Characterization and expression analysis of the expansin gene NnEXPA1 in lotus Nelumbo nucifera
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- Chromosome arm-specific markers from Aegilops searsii permits targeted introgression
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