Biodiversity and screening of halophilic bacteria with hydrolytic and antimicrobial activities from Yuncheng Salt Lake, China
-
Xin Li
und
Ying Hui Yu
Abstract
A total of 152 halophilic bacteria were isolated from Yuncheng Salt Lake, China. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that thirty-four strains were related to the phylum Firmicutes and belonged to three families, Bacillaceae, Clostridiaceae and Staphylococcaceae. The other strains were identified as the members of Halomonadaceae and Idiomarinaceae, which belonged to the phylum γ-Proteobacteria. Nine strains showed <97% similarity of 16S rRNA gene sequence compared to other published species, which might represent novel species. The halophilic isolates exhibited various hydrolytic activities. A total of 74, 15, 70, 18, 23 and 3 strains were found to produce extracellular amylase, protease, lipase, cellulase, pectinase and DNAase, respectively. Most hydrolase-producers were members of the genus Halomonas. Combined hydrolytic activities were shown by some strains. Screening of antimicrobial activity indicated that 3, 6, 15, 12, 15 and 16 of halophilic isolates could inhibit Staphylococcus aureus, Escherichia coli, Candida albicans, Fusarium moniliforme, Fusarium semitectum and Fusarium oxysporum, respectively. Results from the present study indicated that halophilic bacteria may be developed as promising sources for novel biocatalysts or bioactive substances in the field of biotechnology.
References
Baati H., Amdouni R., Gharsallah N., Sghir A. & Ammar E. 2010. Isolation and characterization of moderately halophilic bacteria from Tunisian solar saltern. Curr. Microbiol. 60: 157-161.10.1007/s00284-009-9516-6Suche in Google Scholar PubMed
Benson D.A., Cavanaugh M., Clark K., Karsch-Mizrachi I., Lipman D.J., Ostell J. & Sayers E.W. 2013. GenBank. Nucleic Acids Res. 41: D36-D42.10.1093/nar/gks1195Suche in Google Scholar PubMed PubMed Central
Chen L., Wang G.Y., Bu T., Zhang Y.B., Wang Y.X., Liu M. & Lin X.K. 2010. Phylogenetic analysis and screening of antimicrobial and cytotoxic activities of moderately halophilic bacteria isolated from the Weihai Solar Saltern (China). World. J. Microbiol. Biotechnol. 26: 879-888.10.1007/s11274-009-0247-4Suche in Google Scholar
Chun J., Lee J.H., Jung Y., Kim M., Kim S., Kim B.K. & Lim Y.W. 2007. EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int. J. Syst. Evol. Microbiol. 57: 2259-2261.10.1099/ijs.0.64915-0Suche in Google Scholar PubMed
DasSarma S. & DasSarma P. 2006. Halophiles, Encyclopedia of Life Sciences. Wiley, London.10.1038/npg.els.0004356Suche in Google Scholar
Gao X.B., Wang Y.X., Li Y.L. & Guo Q.H. 2007. Enrichment of fluoride in groundwater under the impact of saline water intrusion at the salt lake area of Yuncheng basin, northern China. Environ. Geol. 53: 795-803.10.1007/s00254-007-0692-zSuche in Google Scholar
Ghozlan H., Deif H., Kandil R.A. & Sabry S. 2006. Biodiversity of moderately halophilic bacteria in hypersaline habitats in Egypt. J. Gen. Appl. Microbiol. 52: 63-72.10.2323/jgam.52.63Suche in Google Scholar PubMed
Han J., Hou J., Liu H., Cai S., Feng B., Zhou J. & Xiang H. 2010. Wide distribution among halophilic archaea of a novel polyhydroxyalkanoate synthase subtype with homology to bacterial type III synthases. Appl. Environ. Microbiol. 76: 7811-7819.10.1128/AEM.01117-10Suche in Google Scholar PubMed PubMed Central
Kumar S., Nei M., Dudley J. & Tamura K. 2008. MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief. Bioinform. 9: 299-306.10.1093/bib/bbn017Suche in Google Scholar PubMed PubMed Central
Lee J.H., Shin H.H., Lee D.S., Kwon K.K., Kim S.J. & Lee H.K. 1999. Bacterial diversity of culturable isolates from seawater and a marine coral, Plexauridae sp., near Mun-Sum, Cheju- Island. J. Microbiol. 37: 193-199.Suche in Google Scholar
Li X. & Yu H.Y. 2011. Extracellular production of β-amylase by a halophilic isolate, Halobacillus sp. LY9. J. Ind. Microbiol. Biotechnol. 38: 1837-1843.Suche in Google Scholar
Li X., Yu H.Y., Liu X.X. & Sun X. 2011. Production and characterization of a novel extracellular metalloproteinase by a newly isolated moderate halophile, Halobacillus sp. LY6. Folia. Microbiol. 56: 329-334.Suche in Google Scholar
Litchfield C.D. & Gillevet P.M. 2002. Microbial diversity and complexity in hypersaline environments: a preliminary assessment. J. Ind. Microbiol. Biotechnol. 28: 48-55.10.1038/sj/jim/7000175Suche in Google Scholar PubMed
Liu H., Zhou Y., Liu R., Zhang K.Y. & Lai R. 2009. Bacillus solisalsi sp. nov., a halotolerant, alkaliphilic bacterium isolated from soil around a salt lake. Int. J. Syst. Evol. Microbiol. 59: 1460-1464.Suche in Google Scholar
Margesin R. & Schinner F. 2001. Potential of halotolerant and halophilic microorganisms for biotechnology. Extremophiles 5: 73-83.10.1007/s007920100184Suche in Google Scholar
Mellado M.E. & Ventosa A. 2003. Biotechnological potential of moderately and extremely halophilic microorganisms, pp. 233-256. In: Barredo J.L. (ed.) Microorganisms for Health Care, Food and Enzyme Production. Research Signpost, Kerala.Suche in Google Scholar
Oren A. 2002. Diversity of halophilic microorganisms: environments, phylogeny, physiology, and applications. J. Ind. Microbiol. Biotechnol. 28: 56-63.Suche in Google Scholar
Roberts M.F. 2004. Osmoadaptation and osmoregulation in archaea: update 2004. Front. Biosci. 9: 1999-2019.10.2741/1366Suche in Google Scholar
Rohban R., Amoozegar M.A. & Ventosa A. 2009. Screening and isolation of halophilic bacteria producing extracellular hydrolyses from Howz Soltan Lake, Iran. J. Ind. Microbiol. Biotechnol. 36: 333-340.10.1007/s10295-008-0500-0Suche in Google Scholar
Sadfi-Zouaoui N., Essghaier B., Hajlaoui M.R., Fardeau M.L., Cayaol J.L., Ollivier B. & Boudabous A. 2008. Ability of moderately halophilic bacteria to control grey mould disease on tomato fruits. J. Phytopathol. 156: 42-52.10.1111/j.1439-0434.2007.01329.xSuche in Google Scholar
Saitou N. & Nei M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406-425.Suche in Google Scholar
Sánchez-Porro C., Martín S., Mellado E. & Ventosa A. 2003. Diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes. J. Appl. Microbiol. 94: 295-300.10.1046/j.1365-2672.2003.01834.xSuche in Google Scholar
Smibert R.M. & Krieg N.R. 1994. Phenotypic characterization, pp 607-654. In: Gerhardt P., Murray R.G.E., Wood W.A. & Krieg N.R. (eds) Methods for General and Molecular Bacteriology. American Society for Microbiology, Washington DC.Suche in Google Scholar
Thompson J.D., Higgins D.G. & Gibson T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic. Acids Res. 22: 4673-4680.Suche in Google Scholar
Urakawa H., Kita-Tsukamoto K. & Ohwada K. 1999. Microbial diversity in marine sediments from Sagami Bay and Tokyo Bay, Japan, as determined by 16S rRNA gene analysis. Microbiology 145: 3305-3315.Suche in Google Scholar
Ventosa A. 2006. Unusual microorganisms from unusual habitats: hypersaline environments, pp 223-253. In: Logan N.A., Lappin-Scott H.M. & Oyston P.C.F. (eds) Prokaryotic Diversity - Mechanism and Significance. Cambridge University Press, Cambridge, London.10.1017/CBO9780511754913.015Suche in Google Scholar
Ventosa A., Nieto J.J. & Oren A. 1998. Biology of moderately halophilic aerobic bacteria. Microbiol. Mol. Biol. Rev. 62: 504-544.10.1128/MMBR.62.2.504-544.1998Suche in Google Scholar
Yang L., Tan R. & Wang Q. 2002. Antifungal cyclopeptides from Halobacillus litoralis YS3106 of marine origin. Tetrahedron Lett. 43: 6545-6548.10.1016/S0040-4039(02)01458-2Suche in Google Scholar
Yeon S.H., Jeong W.J. & Park J.S. 2005. The diversity of culturable organotrophic bacteria from local solar salterns. J. Microbiol. 43: 1-10.Suche in Google Scholar
Yoon J.H., Kim H., Kim S.B., Kim H.J., Kim W.Y., Lee S.T., Goodfellow M. & Park Y.H. 1996. Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int. J. Syst. Bacteriol. 46: 502-505.10.1099/00207713-46-2-502Suche in Google Scholar
© 2015
Artikel in diesem Heft
- Biodiversity and screening of halophilic bacteria with hydrolytic and antimicrobial activities from Yuncheng Salt Lake, China
- Prokaryotic expression and characterization of a keratinolytic protease from Aspergillus niger
- ABA-dependent sucrose regulation of antioxidant metabolism in wheat cultivars varying in ABA-sensitivity
- Identification of salt tolerant Acacia species for saline land utilisation
- Phylogenetic relationships among Elymus and related diploid genera (Triticeae: Poaceae) based on nuclear rDNA ITS sequences
- Adaptations to oxidative stress in Zea mays roots under short-term Pb2+ exposure
- Zinc alleviates cadmium effects on growth, membrane lipid biosynthesis and peroxidation in Solanum lycopersicum leaves
- Comparison of the floodplain forest floristic composition of two riparian corridors: species richness, alien species and the effect of water regime changes
- Red list of ferns and flowering plants of Slovakia, 5th edition (October 2014)
- Distribution and abundance of Hemolivia mauritanica (Apicomplexa: Haemogregarinidae) and its vector Hyalomma aegyptium in tortoises of Iran
- First record of mite genus Ledermuelleriopsis (Acari: Stigmaeidae) in South America with redescription of L. verricula from Brazil
- The influence of agricultural management on the structure of ground beetle (Coleoptera: Carabidae) assemblages
- Insect community on Jurinea cyanoides (Asteraceae), a plant species protected under NATURA 2000
- Chlorella pyrenoidosa, young barley and fruit peel polyphenols in rat breast cancer model – the effects on plasma lipid metabolism
- Increased endogenous serotonin level in diabetic conditions may lead to cardiac valvulopathy via reactive oxygen species regulation
- Parasitic helminths in grey heron (Ardea cinerea) chicks
Artikel in diesem Heft
- Biodiversity and screening of halophilic bacteria with hydrolytic and antimicrobial activities from Yuncheng Salt Lake, China
- Prokaryotic expression and characterization of a keratinolytic protease from Aspergillus niger
- ABA-dependent sucrose regulation of antioxidant metabolism in wheat cultivars varying in ABA-sensitivity
- Identification of salt tolerant Acacia species for saline land utilisation
- Phylogenetic relationships among Elymus and related diploid genera (Triticeae: Poaceae) based on nuclear rDNA ITS sequences
- Adaptations to oxidative stress in Zea mays roots under short-term Pb2+ exposure
- Zinc alleviates cadmium effects on growth, membrane lipid biosynthesis and peroxidation in Solanum lycopersicum leaves
- Comparison of the floodplain forest floristic composition of two riparian corridors: species richness, alien species and the effect of water regime changes
- Red list of ferns and flowering plants of Slovakia, 5th edition (October 2014)
- Distribution and abundance of Hemolivia mauritanica (Apicomplexa: Haemogregarinidae) and its vector Hyalomma aegyptium in tortoises of Iran
- First record of mite genus Ledermuelleriopsis (Acari: Stigmaeidae) in South America with redescription of L. verricula from Brazil
- The influence of agricultural management on the structure of ground beetle (Coleoptera: Carabidae) assemblages
- Insect community on Jurinea cyanoides (Asteraceae), a plant species protected under NATURA 2000
- Chlorella pyrenoidosa, young barley and fruit peel polyphenols in rat breast cancer model – the effects on plasma lipid metabolism
- Increased endogenous serotonin level in diabetic conditions may lead to cardiac valvulopathy via reactive oxygen species regulation
- Parasitic helminths in grey heron (Ardea cinerea) chicks
