Startseite Medizin First molecular identification of an agent of diplostomiasis, Diplostomum pseudospathaceum (Niewiadomska 1984) in the United Kingdom and its genetic relationship with populations in Europe
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First molecular identification of an agent of diplostomiasis, Diplostomum pseudospathaceum (Niewiadomska 1984) in the United Kingdom and its genetic relationship with populations in Europe

  • Egie Elisha Enabulele EMAIL logo , Agnes Ogheneruemu Awharitoma , Scott P. Lawton und Ruth S. Kirk
Veröffentlicht/Copyright: 4. Juli 2018
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Acta Parasitologica
Aus der Zeitschrift Acta Parasitologica Band 63 Heft 3

Abstract

Trematode genus Diplostomum comprises of parasitic species which cause diplostomiasis, the ‘white eye’ disease in fish and heavy infection can result in mortality. The increasing availability of DNA sequences of accurately identified Diplostomum species on public data base presently enables the rapid identification of species from novel sequences. We report the first molecular evidence of the occurrence of D. pseudospathaceum in the United Kingdom. Two gene regions, nuclear internal transcribed spacer cluster (ITS1-5.8S-ITS2) and mitochondrial cytochrome c oxidase subunit 1 (cox1) of cercariae from infected aquatic snails, Lymnaea stagnalis collected in several locations in Southern England were sequenced. Phylogenetic analysis based on both sequenced genes revealed that the novel sequences were D. pseudospathaceum. Molecular diversity analysis of published D. pseudospathaceum cox1 sequences from seven countries in Europe and the novel sequences from the present study revealed high diversity, but low nucleotide divergence and a lack of gene differentiation between the populations. Haplotype network analysis depicted a star-like pattern and revealed a lack of geographic structure in the population. Fixation indices confirmed gene flow between populations and we suspect high levels of dispersal facilitated by highly mobile second intermediate (fish) and definitive (piscivorous birds) host may be driving gene flow between populations. Neutrality tests and mismatch distribution indicated recent population growth/expansion for D. pseudospathaceum in Europe.

Keywords: Diplostomum pseudospathaceum; Lymnaea stagnalis; United Kingdom; Europe; molecular diversity; population genetics

Acknowledgements

The co-operation of the Four Seasons Hotel Hampshire, Godalming Angling Club and Nature England are appreciated for allowing snail sampling in the sites visited. This research was made possible by funding from the Tertiary Education Trust Fund of Nigeria for PhD studentship to the corresponding author at Kingston University London.

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Received: 2017-10-30
Revised: 2018-02-20
Accepted: 2018-03-02
Published Online: 2018-07-04
Published in Print: 2018-09-25

© 2018 W. Stefański Institute of Parasitology, PAS

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https://doi.org/10.1515/ap-2018-0054
Schlagwörter für diesen Artikel
Diplostomum pseudospathaceum; Lymnaea stagnalis; United Kingdom; Europe; molecular diversity; population genetics

Artikel in diesem Heft

  2. Human case of Fasciola gigantica-like infection, review of human fascioliasis reports in Nepal, and epidemiological analysis within the South Central Asia
  4. First molecular identification of an agent of diplostomiasis, Diplostomum pseudospathaceum (Niewiadomska 1984) in the United Kingdom and its genetic relationship with populations in Europe
  6. The discovery of Lepeophtheirus acutusHeegaard, 1943 (Copepoda: Caligidae) from two new elasmobranch hosts in the Mediterranean Sea, and a comparative redescription of Lepeophtheirus rhinobati Luque, Chaves et Cezar, 1998
  8. Endoparasites in Limnonectes magnus (Anura, Dicroglossidae) from Samar Island, Philippines with description of a new species of Aplectana (Nematoda, Cosmocercidae)
  10. Morphological and molecular study of Neorhadinorhynchus nudus (Harada, 1938) (Acanthocephala: Cavisomidae) from Auxis thazard Lacepede (Perciformes: Scombridae) in the South China Sea
  12. Alveolar echinococcosis in a dog; analysis of clinical and histological findings and molecular identification of Echinococcus multilocularis
  14. Morphological and phylogenetic characterization of a novel Unicauda species, infecting the kidney of Astyanax altiparanae (Teleostei: Characidae) in Brazil
  16. Systemic oxidative stress in Suffolk and Santa Ines sheep experimentally infected with Haemonchus contortus
  18. Molecular characterization of Theileria spp. in livestock and the first report on the occurrence of Theileria sp. OT3 in Iran
  20. Molecular phylogenetic position of Haplometroides intercaecalis (Digenea, Plagiorchiidae)
  22. Ten new species of BrueeliaKéler, 1936 (Phthiraptera: Ischnocera: Philopteridae) from nuthatches (Aves: Passeriformes: Sittidae), tits and chickadees (Paridae), and goldcrests (Regulidae)
  24. A new coccidian parasite (Apicomplexa: Eimeriidae: Eimeria) from the southern black racer, Coluber constrictor priapus (Reptilia: Ophidia: Colubridae) from Arkansas, USA
  26. Assessment of oxidative/nitrosative stress biomarkers and DNA damage in Haemonchus contortus, following exposure to zinc oxide nanoparticles
  28. Descriptions of Acanthocephalus parallelcementglandatus (Echinorhynchidae) and Neoechinorhynchus (N.) pennahia (Neoechinorhynchidae) (Acanthocephala) from amphibians and fish in Central and Pacific coast of Vietnam, with notes on N. (N.) longnucleatus
  30. Morphological and molecular characterization of Paramphistomum epiclitum of small ruminants
  32. Further study on Procamallanus (Spirocamallanus) pintoi (Kohn et Fernandes, 1988) (Nematoda: Camallanidae) in Corydoras paleatus and Corydoras micracanthus (Siluriformes: Callichthyidae) from Salta, Argentina, with a key to congeneric species from Neotropical Realm
  34. Screening of Cercopithifilaria bainae and Hepatozoon canis in ticks collected from dogs of Northeastern Brazil
  36. Epidemiological and genetic characterization of larval stages of Fasciola gigantica in snail intermediate hosts in Karnataka State, India
  38. Does apicortin, a characteristic protein of apicomplexan parasites and placozoa, occur in Eumetazoa?
  40. Comparison of sensitivity of two primer sets for the detection of Toxoplasma gondii DNA in wildlife
  41. Research Note
  42. Ascaridia galli isolates with ITS1-5.8rRNA-ITS2 fragment homologous to Ascaridia columbae
  43. Research Note
  44. Anti-Neospora caninum antibodies in feral cats on the Island of Fernando de Noronha, Brazil
  45. Research Note
  46. First molecular evidence of Thelohanellus wallagoiSarkar, 1985 (Myxozoa) from economically important food fish, freshwater shark Wallago attu (Siluridae) in India
  47. Research Note
  48. First identification of Echinococcus multilocularis in golden jackals in Croatia
  49. Case Report
  50. Dirofilaria repens infection as a cause of intensive peripheral microfilariemia in a Polish patient: process description and cases review
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