Ascaridia galli isolates with ITS1-5.8rRNA-ITS2 fragment homologous to Ascaridia columbae
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Joanna Urbanowicz
Abstract
Ascaridia (A.) galli is one of the most commonly occurring nematodes in poultry worldwide, often in hens and broiler chickens. The infection with Ascaridia galli in free-range chickens was even 70%. There is not much information about A. galli genetic features. The present study was conducted to assess the genetic diversity of A. galli isolated from hens in Poland by analyzing the nucleotide sequence of the region ITS1-5.8rRNA-ITS2 and to define its homology within the family Ascaridiidae. Adult A. galli were collected from the intestines of naturally infected hens from two flocks of free-run laying hens from the Wielkopolska region in Poland. From all parasites an identical ITS1-5.8rRNA-ITS2 sequence was obtained, which was homologous in 99% with A. columbae (JQ995321.1) sequence. The high homology sequences of A. galli (KX683286) from Poland and A. columbae (JQ995321.1) isolate from the USA, support the observations of other authors suggesting that A. galli and A. columbae might be closely related. It is the first whole ITS1-5.8rRNA-ITS2 of A. galli in the GenBank database, so there is not enough data for detailed phylogenetic analysis of A. galli. Detailed genetic analysis is necessary to get better insight into the birds’ Ascaridia species.
Abbreviations
- ITS
internal transcribed spacers (ITS)
Acknowledgements
Research supported by Wroclaw Center of Biotechnology, programme The Leading National Research Center (KNOW) for years 2014–2018.
References
Adamson M. 1987. Phylogenetic analysis of the higher classification of the Nematoda. Canadian Journal of Zoology, 65, 1478–148210.1139/z87-230Suche in Google Scholar
Anderson T.J. 2001. The dangers of using single locus markers in parasite epidemiology: Ascaris as a case study. Trends in Parasitology, 17,183–18810.1016/S1471-4922(00)01944-9Suche in Google Scholar
Blaxter ML., De Ley P., Garey J. R., Liu L.X., Scheldeman P., Vierstraete A., et al. 1998. A molecular evolutionary framework for the phylum Nematoda. Nature, 392, 71–75. 10.1038/32160Suche in Google Scholar
Bazh E.K. 2013. Molecular characterization of Ascaridia galli infecting native chickens in Egypt. Parasitology Research, 112, 3223–3227. 10.1007/s00436-013-3498-9.Suche in Google Scholar
Chadfield M.S., Permin A., Nansen P., Bisgaard M. 2001. Investigation of the parasitic nematode Ascaridia galli as a potential vector for Salmonella dissemination in broiler poultry. Parasitology Research, 87, 317–32510.1007/PL00008585Suche in Google Scholar
Chilton N.B., Gasser R.B., Beveridge I. 1997. Phylogenetic relationships of Australian strongyloid nematodes inferred from ribosomal DNA sequence data. International Journal of Parasitology, 27, 1481–149410.1016/S0020-7519(97)00134-3Suche in Google Scholar
Długosz E., Wiśniewski M. 2006. Molecular diagnostic of parasites using rRNA gene sequence. Wiadomości Parazytologiczne, 52, 263–269Suche in Google Scholar
Everett E., Hwang J.C., Hwang W. 1914. The Life Cycle and Morphology of Ascaridia columbae (Gmelin, 1790) Travassos, (Nematoda:Ascarididae) in the Domestic Pigeon (Columba livia domestica). Journal of Parasitology, 50, 131–13710.2307/3276047Suche in Google Scholar
Höglund J., Morrison D.A., Engström A., Nejsum P., Jansson D.S. 2012. Population genetic structure of Ascaridia galli re-emerging in non-caged laying hens. Parasites and Vectors, 20, 5–9. 10.1186/1756-3305-5-97Suche in Google Scholar PubMed PubMed Central
Inglis W.G. 1983. An outline classification on the Phylum Nematoda. Australian Journal of Zoology, 31, 243–25510.1071/ZO9830243Suche in Google Scholar
Lin Q., Li H.M., Gao M., Wang X.Y., Ren W.X, Cong M.M., et al. 2012. Characterization of Baylisascaris schroederi from Qinling subspecies of giant panda in China by the first internal transcribed spacer (ITS-1) of nuclear ribosomal DNA. Parasitology Research, 110, 1297–1303. 10.1007/s00436-011-2618-7Suche in Google Scholar PubMed
Liu G.H., Shao R., Li J.Y., Zhou D.H., Li H., Zhu X.Q. 2013. The complete mitochondrial genomes of three parasitic nematodes of birds: a unique gene order and insights into nematode phylogeny. BMC Genomics, 21, 414. 10.1186/1471-2164-14-414Suche in Google Scholar PubMed PubMed Central
Luna-Olivares L.A., Ferdushy T., Kyvsgaard N.C., Nejsum P., Thamsborg S.M., Roepstorff A. et al. 2012. Localization of Ascaridia galli larvae in the jejunum of chickens three days post infection. Veterinary Parasitology, 185, 186–193. 10.1007/s00436-012-3079-3Suche in Google Scholar PubMed
Magwisha H., Kassuku A., Nyvsgaard N., Permin A. 2002. A comparison of the prevalance and burdens of helminth infections in growers and adult free-range chickens. Tropical Animal Health Production, 34, 205–21410.1023/A:1015278524559Suche in Google Scholar
Martin-Pacho J.R., Montoya M.N., Arangna T., Toro C., Morchon C., Marcos-Atxutegi C. et al. 2005. A coprological and serological survery for the prevalence of Ascaridia spp. in Laying Hens. Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health, 52, 238–242. 10.1111/j.1439-0450.2005.00853.xSuche in Google Scholar PubMed
Permin, A., Bisgaard M., Frandsen F., Pearman M., Kold J., Nansen P. 1999. Prevalence of gastrointestinal helminths in different poultry production systems. British Poultry Science, 40, 439–443. 10.1080/00071669987179Suche in Google Scholar PubMed
Permin A., Christensen J.P, Bisgaard M. 2006. Consequences of concurrent Ascaridia galli and Escherichia coli infections in chickens. Acta Veterinaria Scandinavica, 47, 43–5410.1186/1751-0147-47-43Suche in Google Scholar PubMed PubMed Central
Ramadan H.H., Abouznada N.Y. 1992. Morphology and life history of Ascaridia galli in the Domestic Fowl that are raised in Jeddah. Journal of King Saud University – Science, 4, 87–9910.4197/Sci.4-1.9Suche in Google Scholar
Schnare M.N., Collings J.C., Spencer D.F, Gray. M.W. 2000. The 28S-18S rDNA intergenic spacer from Crithidia fasciculata: repeated sequences, length heterogeneity, putative processing sites and potential interactions between U3 small nucleolar RNA and the ribosomal RNA precursor. Nucleic Acids Research, 28, 3452–346110.1093/nar/28.18.3452Suche in Google Scholar PubMed PubMed Central
Szelągiewicz, M., Sokół R. 1991. Parasites of poultry in a small on suburb raisings. Medycyna Weterynaryjna, 47, 208–209Suche in Google Scholar
Veldman G.M., Klootwijk J., Van Heerikhuizen H., Planta R.J. 1981. The nucleotide sequence of the intergenic region between the 5.8S and 26S rRNA genes of the yeast ribosomal RNA operon. Possible implications for the interaction between 5.8S and 26S rRNA and the processing of the primary transcript. Nucleic Acids Research, 9, 4847–486210.1093/nar/9.19.4847Suche in Google Scholar PubMed PubMed Central
Zhao W.T., Guo Y.N., Zhang L.P., Li L. 2016. Ultrastructure of Ascaridia galli (Schrank, 1788) (Nematoda: Ascaridida) from the endangered green peafowl Pavo muticus Linnaeus (Galliformes: Phasianidae). Acta Parasitolologica, 61, 66–73. 10.1515/ap-2016-0007Suche in Google Scholar PubMed
© 2018 W. Stefański Institute of Parasitology, PAS
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