Startseite Medizin Isolation, identification and characterization of the nematophagous fungus Arthrobotrys (Monacrosporium) sinense from China
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Isolation, identification and characterization of the nematophagous fungus Arthrobotrys (Monacrosporium) sinense from China

  • Ya-Juan Xue , Er-Le Li , Cai-Xia Jing , Li Ma und Kui-Zheng Cai EMAIL logo
Veröffentlicht/Copyright: 13. April 2018
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Acta Parasitologica
Aus der Zeitschrift Acta Parasitologica Band 63 Heft 2

Abstract

With the development of anthelmintic resistance of parastic nematodes, it is necessary to isolate and study nematophagous fungi to screen out the native isolates for their potential in the biocontrol of domestic animal nematodosis. This study aimed to isolate the Arthrobotrys sinense (Monacrosporium sinense) of nematophagous fungus, to characterize representative molecular isolates using scanning electron microscope (SEM), and to determine the effect of the temperature and pH values on radial growth of the isolate. Five isolates were isolated from 1532 samples of different types, and their occurrence frequencies were 0.32% of the total samples. They were identified as A. sinense by means of morphology and the sequence of the 5.8S, 18S, and 28S rDNA, as well as internal transcribed spacers 1 and 2. The isolate NBS003 could grow from 11°C to 35°C and had optimal growth at 30°C. The isolate could grow at pH 4 to 11, and its optimal value was obtained at pH 9. SEM results showed that 6 h after their addition, the second stage larvae (L2) and the third stage infective larvae (L3) of Haemonchus contortus were captured. L2 and L3 were penetrated by the fungus at 18 and 24 h post-capture, respectively. L2 and L3 were completely digested at 84 and 90 h post-capture, respectively. The NBS003 of the A. sinense should have a certain potential to be used for capturing the free-living stage of nematodes in sheep.

Keywords: Nematophagous fungi; Arthrobotrys sinense (Monacrosporium sinense); Haemonchus contortus; rDNA; scanning electron microscope

  1. Conflict of interest

    Conflict of interest statement: All authors declare no financial or commercial conflicts of interest.

Acknowledgements

We gratefully acknowledge the financial support of the Science and Technology Program Projects in Yan’an (2016KS-12) and the Key Cultivation Project of the Central University (31920170159).

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Received: 2018-1-3
Revised: 2018-1-29
Accepted: 2018-1-31
Published Online: 2018-4-13
Published in Print: 2018-6-26

© 2018 W. Stefański Institute of Parasitology, PAS

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https://doi.org/10.1515/ap-2018-0037
Schlagwörter für diesen Artikel
Nematophagous fungi; Arthrobotrys sinense (Monacrosporium sinense); Haemonchus contortus; rDNA; scanning electron microscope

Artikel in diesem Heft

  2. Morphological, histological and molecular characterization of Myxobolus kingchowensis and Thelohanellus cf. sinensis infecting gibel carp Carassius auratus gibelio (Bloch, 1782)
  4. Cystic echinococcosis in animals and humans of Maharashtra State, India
  6. Structural predication and antigenic analysis of Toxoplasma gondii ROP20
  8. Molecular cloning of enolase from Trichinella spiralis and the protective immunity in mice
  10. A new species of Cacatuocotyle (Monogenea, Dactylogyridae) parasitizing Astyanax spp. (Characiformes, Characidae) from Brazil, including molecular data and a key to species identification
  12. Entamoeba histolytica L220 induces the in vitro activation of macrophages and neutrophils and is modulated by neurotransmitters
  14. Characterization of the complete mitochondrial genome of Metastrongylus salmi (M. salmi) derived from Tibetan pigs in Tibet, China
  16. Balantioides coli: morphological and ultrastructural characteristics of pig and non-human primate isolates
  18. Genetic diversity of Taenia hydatigena in the northern part of the West Bank, Palestine as determined by mitochondrial DNA sequences
  20. Patterns of the parasite communities in a fish assemblage of a river in the Brazilian Amazon region
  22. Histological description of Cercaria etgesiiShchenkov, 2017 daughter sporocysts (Trematoda: Pleurogenidae): compartmentalization of the brood cavity and germinal mass morphology
  24. Isolation, identification and characterization of the nematophagous fungus Arthrobotrys (Monacrosporium) sinense from China
  26. First molecular characterization and morphological aspects of Sarcocystis fusiformis infecting water buffalo Bubalus bubalis in Egypt
  28. Prevalence and risk factors associated with Tritrichomonas foetus infection in cattle in the state of Paraíba, Brazil
  30. The development of Neoechinorhynchus buttnerae (Eoacanthocephala: Neoechinorhynchidae) in its intermediate host Cypridopsis vidua in Brazil
  32. Primary culture of cat intestinal epithelial cells in vitro and the cDNA library construction
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  47. Case report
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  53. Erratum
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