Abstract
Tritrichomonas foetus, a parasite of cattle reproductive system, has been recently discovered as a cause of disease in cats in many countries. T. foetus infects and colonizes cat’s ileum, caecum, colon and can lead to enteritis. This paper presents the first clinical case of cat intestinal trichomonosis caused by T. foetus in Poland. The material for this study was a smear collected from a 6-month-old male British Shorthair cat. The presence of parasitic protozoan was determined via microscopic examination and confirmed by amplification of T. foetus rDNA using polymerase chain reaction (PCR) technique. In the first PCR reaction, a DNA of Trichomonadidae was identified and in the second PCR, T. foetus was detected. The T. foetus positive products from the second PCR reaction were sequenced. Interpretation of the sequencing results of obtained amplicons by comparing them with the GenBank database proved that the causative agent, in this case, was T. foetus.
References
Bisset S.A., Gowan R.A., O’Brien C.R., Stone M.R., Gookin J.L. 2008. Feline diarrhoea associated with Tritrichomonas cf. foetus and Giardia co-infection in an Australian cattery. Australian Veterinary Journal, 86, 440-443. DOI:10.1111/j.1751-0813.2008.00356.x 10.1111/j.1751-0813.2008.00356.xSearch in Google Scholar PubMed
BonDurant R.H., Campero C.M., Anderson M.L., Hoosear K.A.. 2003. Detection of Tritrichomonas foetus by polymerase chain reaction in culture isolates, cervicovaginal mucus, and formalin-fixed tissues from infected heifers and fetuses. Journal Veterinary Diagnostic Investigation, 15, 579-58410.1177/104063870301500613Search in Google Scholar PubMed
Carvalho K.P., Gadelha A.P. 2007. Effects of three benzimidazoles on growth, general morphology and ultrastructure of Tritrichomonas foetus. FEMS Microbiology Letters, 275, 292-300. DOI: 10.1111/j.1574-6968.2007.00897.x 10.1111/j.1574-6968.2007.00897.xSearch in Google Scholar PubMed
Da Silva N.S., Ribeiro Cde M., Machado A.H., Pacheco-Soares C. 2007. Ultrastructural changes in Tritrichomonas foetus after treatments with A1PcS4 and photodynamic therapy. Veterinary Parasitology, 146, 175-181. DOI:10.1016/j.vetpar.2007.02.00610.1016/j.vetpar.2007.02.006Search in Google Scholar PubMed
Felleisen R.S.J., Lambelet N., Bachmann P., Nicolet J., Muller N., Gottstein B. 1998. Detection of Tritrichomonas foetus by PCR and DNA Enyzme Imunnoassay Based on rRNA Gene Unit Sequences. Journal Clinical Microbiology, 36, 513-51910.1128/JCM.36.2.513-519.1998Search in Google Scholar PubMed PubMed Central
Foster D.M., Gookin J.L., Poore M.F., Stebbins M.E., Levy M.G. 2004 Outcome of cats with diarrhoea and Tritrichomonas foetus infection. Journal American Veterinary Medical Association, 225, 888-89210.2460/javma.2004.225.888Search in Google Scholar PubMed
Frey C.F., Schild M., Hemphill A., Stunzi P., Muller N., Gottstein B., Burgener I.A.. 2009. Intestinal Tritrichomonas foetus infection in cats in Switzerland detected by in vitro cultivation and PCR. Parasitology Research, 104, 783-788. DOI: 10.3347/kjp.2010.48.3.24710.3347/kjp.2010.48.3.247Search in Google Scholar PubMed PubMed Central
Gookin J.L., Breitschwerdt E.B., Levy M.G., Gager R.B., Benrud J.G. 1999. Diarrhea associated with trichomonosis in cats. Journal American Veterinary Medical Association, 215, 1450-4Search in Google Scholar
Granger B.L., Warwood S.J., Benchimol M., De Souza W. 2000. Transient invagination of flagella by Tritrichomonas foetus. Parasitology Research, 86, 699-709. DOI: 10.1007/PL 00008555Search in Google Scholar
Gray S.G., Hunter S.A., Stone M.R., Gookin J.L. 2010. Assessement of reproductive tract disease in cats at risk for Tritrichomonas foetus infection. AVMA - American Journal of Veterinary Research, 71, 76-81. DOI: 10.2460/ajvr.71.1.7610.2460/ajvr.71.1.76Search in Google Scholar PubMed
Gunn-Moore D.A., McCann T.M., Reed N., Simpson K.E., Tennant B. 2007. Prevalence of Tritrichomonas foetus infection in cats with diarrhoea in the UK. Journal Feline Medicine Surgery, 9, 214-21810.1016/j.jfms.2007.01.003Search in Google Scholar PubMed
Hammond D.M., Bartlett D.E. 1943. The distribution of Trichomonas foetus in the preputial cavity of infected bulls. American Journal Veterinary Research, 4, 143-149Search in Google Scholar
Holliday M., Deni D., Gunn-Moore D.A.. 2009. Tritrichomonas foetus infection in cats with diarrhoea in a rescue colony in Italy. Journal of Feline Medicine and Surgery, 11, 131-134. DOI: 10.1016/j.jfms.2008.06.00410.1016/j.jfms.2008.06.004Search in Google Scholar PubMed
Honigberg B.M. 1978. Parasitic protozoa. II. Intestinal flagellates, histomonads, trichomonads, amoeba, opalinids and ciliates. Academic Press, Inc New York, N.Y.Search in Google Scholar
Klein B., Langbein-Detsch I., Muller E., Heusinger A. 2010. Pravalenz von Tritrichomonas foetus in Kotproben von Katzen mit Durchfall aus Deutschland. Kleintiermedizin, 48, 247-251Search in Google Scholar
Kuehner K.A., Marks S.L., Kass P.H., Sauter L.C., Grahn R.A., Barutzki D., Hartman K. 2011. Tritrichomonas foetus infection in purebred cats in Germany: prevalence of clinical signs and the role of co-infection with other enteroparaistes. Journal Feline Medicine Surgery, 13, 251-258. DOI: 10.1016/ j.jfms.2010.12.00210.1016/j.jfms.2010.12.002Search in Google Scholar PubMed
Lim S., Park S.I., Ahn K.S., Oh D.S., Ryu J.S., Shin S.S. 2010. First Report of Feline Intestinal Trichomoniasis Caused by Tritrichomonas foetus in Korea. Korean Journal Parasitology, 48, 247-251. DOI: 10.3347/kjp.2010.48.3.24710.3347/kjp.2010.48.3.247Search in Google Scholar PubMed PubMed Central
Profizi C., Cian A., Meloni D., Hugonnard M., Lambert V., Groud K., Gagon A.C., Viscogliosi E., Zenner L. 2013. Prevalence of Tritrichomonas foetus infections in French catteries. Veterinary Parasitology, 196, 50-55. DOI: 10.1016/j.vetpar.2013.01.02110.1016/j.vetpar.2013.01.021Search in Google Scholar PubMed
Stauffer S.H., Birkenheuer A.J., Levy M.G., Marr H., Gookin J.L. 2008. Evaluation of four DNA extraction methods for the detection of Tritrichomonas foetus in feline stool specimens by polymerase chain reaction. Journal of Veterinary Diagnostic Investigation, 20, 639-641. DOI:10.1177/10406387080200051810.1177/104063870802000518Search in Google Scholar PubMed
Stockdale H.D., Dillon A.R., Newton J.C., Bird R.C, Bondurant R.H., Deinnocentes P., Barney S., Bulter J., Land T., Spencer J.A., Linday D.S., Blagburn B.L. 2008. Experimental infection of cats (Felis catus) with Tritrichomonas foetus isolated from cattle. Veterinary Parasitology, 154, 156-161. DOI: 10.1016/j.vetpar.2008.02.02410.1016/j.vetpar.2008.02.024Search in Google Scholar PubMed
Stockdale H.D., Givens M.D., Dykstra C., Blagburn L. 2009. Tritrichomonas foetus infections in surveyed pet cats. Veterinary Parasitology, 160: 13-17. DOI: 10.1016/j.vetpar.2008.10.09110.1016/j.vetpar.2008.10.091Search in Google Scholar PubMed
Tysnes K., Gjerde B., Nodtvedt A., Skancke E. 2011. A cross-sectional study of Tritrichomonas foetus infection among healthy cats at shows in Norway. Acta Veterinaria Scandinavica, 53(39), 1-6. DOI: 10.1186/1751-0147-53-3910.1186/1751-0147-53-39Search in Google Scholar PubMed PubMed Central
Walden H.S, Dykstra Ch., Dillon A., Rodning S., Givens D., Bird R., Lindsay D. 2013. A new species of Tritrichomonas (Sarcomastigophora: Trichomonida) from the domestic cat (Felis catus). Parasitology Research, 112, 2227-2235. DOI: 10.1007/s00436-013-3381-810.1007/s00436-013-3381-8Search in Google Scholar PubMed
Walker R.L., Hayes D.C., Sawyer S.J., Nordhausen R.W., Van Hoosear K.A., BonDurant R.H. 2003. Comparison of the 5,8S rRNA gene and internal transcribed spacer regions of trichomonadid protozoa recovered from the bovine preputial cavity. Journal Veterinary Diagnostic Investigation, 15, 14-2010.1177/104063870301500104Search in Google Scholar PubMed
Xenoulis P.G. 2011. Tritrichomonas foetus: a new cause of diarrhea in cats in Greece. Journal of Hell Veterinary Medicine Society, 62(2), 132-140. DOI: 10.1016/j.jfms.2010.05.01010.1016/j.jfms.2010.05.010Search in Google Scholar PubMed
Yaeger M.J., Gookin J.L. 2005. Histologic Features associated with Tritrichomonas foetus- induced Colitis in Domestic Cats. Veterinary Pathology, 42, 797-804 10.1354/vp.42-6-797Search in Google Scholar PubMed
W. Stefański Institute of Parasitology, PAS
Articles in the same Issue
- Sperm transfer in monogenean (platyhelminth) parasites
- A survey for potentially zoonotic gastrointestinal parasites of dogs and pigs in Cambodia
- Tritrichomonas foetus infection in cat – first detection in Poland
- Association between immunoreactivity to Anisakis spp. antigens and high-risk pregnancy
- Stibarokris mariasi sp. nov. – a new quill mite species (Acariformes: Syringophilidae) parasitizing Puffinus pacificus (Gmelin) (Procellariidae) on Johnston Atoll
- Seroprevalence and correlates of Toxoplasma gondii infection in dairy cattle in northwest China
- Mites and internal parasites associated with the common dung beetle Geotrupes (Anoplotrupes) stercorosus (Hartmann in Scriba, 1791) in Poland
- New species of Cosmocercoides (Nematoda; Cosmocercidae) and other helminths in Gonocephalus liogaster (Squamata; Agamidae) from Peninsular Malaysia
- Cryptosporidium identification in HIV-Infected Humans. Experience from Kinshasa, the Democratic Republic of Congo
- The infection of reintroduced ruminants – Bison bonasus and Alces alces – with Anaplasma phagocytophilum in northern Poland
- Molecular studies on larvae of Pseudoterranova parasite of Trichiurus lepturus Linnaeus, 1758 and Pomatomus saltatrix (Linnaeus, 1766) off Brazilian waters
- Coexistence of Borrelia burgdorferi s.l. genospecies within Ixodes ricinus ticks from central and eastern Poland
- Molecular characterization of cystic echinococcosis: First record of G7 in Egypt and G1 in Yemen
- Prevalence of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected from southern Poland
- A new species of Cosmocerca (Nematoda: Cosmocercidae) and other helminths in Cyrtodactylus gubaot (Squamata: Gekkonidae) from the Philippines
- Alveolar echinococcosis: how knowledgeable are primary care physicians and pharmacists in the Franche-Comté region of France?
- Morphologic and molecular characteristics of Sarcocystis atraii n. sp. (Apicomplexa: Sarcocystidae) infecting the common coot (Fulica atra) from Egypt
- Auto-infection by Echinostoma spp. cercariae in Helisoma anceps
- Risk factors of occurrence of Toxoplasma gondii among horses in the state of Alagoas, Brazil
- Cardiopulmonary helminths in foxes from the Pyrenees
- Risk of soil-transmitted helminth infections on agritourism farms in central and eastern Poland
- Evaluating the Babesia bovis infection of cattle in China with enzyme-linked immunosorbent assay (ELISA)
- Production and preliminary evaluation of Trypanosoma evansi HSP70 for antibody detection in Equids
- Malondialdehyde; Lipid peroxidation plasma biomarker correlated with hepatic fibrosis in human Schistosoma mansoni infection
- A new species of the feather mite genus Tyrannidectes Mironov, 2008 (Acariformes: Proctophyllodidae) from tapaculos (Passeriformes: Rhinocryptidae) in Chile
- Recent study on canine vector-borne zoonoses in southern Slovakia – serologic survey
- Redescription of Paraleptus chiloscyllii Yin et Zhang, 1983 (Nematoda: Physalopteridae) from the Arabian carpetshark Chiloscyllium arabicum (Chondrichthyes: Hemiscylliidae) off Iraq
- A New Species of Ligophorus (Monogenea: Dactylogyridae) from the gills of the Flathead Mullet Mugil cephalus (Teleostei: Mugilidae) from Mexico
- Demodex mites as potential etiological factor in chalazion – a study in Poland
- First report of Schulzia travassosi (Nematoda, Trichostrongylina, Molineoidea) for amphibians of the Chaco region in Argentina and proposal of Oswaldocruzia melanostictusi nov. comb.
- First molecular identification of Ascocotyle (Phagicola) longa in its first intermediate host the mud snail Heleobia australis
- Dog’s genotype of Giardia duodenalis in human: first evidence in Europe
Articles in the same Issue
- Sperm transfer in monogenean (platyhelminth) parasites
- A survey for potentially zoonotic gastrointestinal parasites of dogs and pigs in Cambodia
- Tritrichomonas foetus infection in cat – first detection in Poland
- Association between immunoreactivity to Anisakis spp. antigens and high-risk pregnancy
- Stibarokris mariasi sp. nov. – a new quill mite species (Acariformes: Syringophilidae) parasitizing Puffinus pacificus (Gmelin) (Procellariidae) on Johnston Atoll
- Seroprevalence and correlates of Toxoplasma gondii infection in dairy cattle in northwest China
- Mites and internal parasites associated with the common dung beetle Geotrupes (Anoplotrupes) stercorosus (Hartmann in Scriba, 1791) in Poland
- New species of Cosmocercoides (Nematoda; Cosmocercidae) and other helminths in Gonocephalus liogaster (Squamata; Agamidae) from Peninsular Malaysia
- Cryptosporidium identification in HIV-Infected Humans. Experience from Kinshasa, the Democratic Republic of Congo
- The infection of reintroduced ruminants – Bison bonasus and Alces alces – with Anaplasma phagocytophilum in northern Poland
- Molecular studies on larvae of Pseudoterranova parasite of Trichiurus lepturus Linnaeus, 1758 and Pomatomus saltatrix (Linnaeus, 1766) off Brazilian waters
- Coexistence of Borrelia burgdorferi s.l. genospecies within Ixodes ricinus ticks from central and eastern Poland
- Molecular characterization of cystic echinococcosis: First record of G7 in Egypt and G1 in Yemen
- Prevalence of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected from southern Poland
- A new species of Cosmocerca (Nematoda: Cosmocercidae) and other helminths in Cyrtodactylus gubaot (Squamata: Gekkonidae) from the Philippines
- Alveolar echinococcosis: how knowledgeable are primary care physicians and pharmacists in the Franche-Comté region of France?
- Morphologic and molecular characteristics of Sarcocystis atraii n. sp. (Apicomplexa: Sarcocystidae) infecting the common coot (Fulica atra) from Egypt
- Auto-infection by Echinostoma spp. cercariae in Helisoma anceps
- Risk factors of occurrence of Toxoplasma gondii among horses in the state of Alagoas, Brazil
- Cardiopulmonary helminths in foxes from the Pyrenees
- Risk of soil-transmitted helminth infections on agritourism farms in central and eastern Poland
- Evaluating the Babesia bovis infection of cattle in China with enzyme-linked immunosorbent assay (ELISA)
- Production and preliminary evaluation of Trypanosoma evansi HSP70 for antibody detection in Equids
- Malondialdehyde; Lipid peroxidation plasma biomarker correlated with hepatic fibrosis in human Schistosoma mansoni infection
- A new species of the feather mite genus Tyrannidectes Mironov, 2008 (Acariformes: Proctophyllodidae) from tapaculos (Passeriformes: Rhinocryptidae) in Chile
- Recent study on canine vector-borne zoonoses in southern Slovakia – serologic survey
- Redescription of Paraleptus chiloscyllii Yin et Zhang, 1983 (Nematoda: Physalopteridae) from the Arabian carpetshark Chiloscyllium arabicum (Chondrichthyes: Hemiscylliidae) off Iraq
- A New Species of Ligophorus (Monogenea: Dactylogyridae) from the gills of the Flathead Mullet Mugil cephalus (Teleostei: Mugilidae) from Mexico
- Demodex mites as potential etiological factor in chalazion – a study in Poland
- First report of Schulzia travassosi (Nematoda, Trichostrongylina, Molineoidea) for amphibians of the Chaco region in Argentina and proposal of Oswaldocruzia melanostictusi nov. comb.
- First molecular identification of Ascocotyle (Phagicola) longa in its first intermediate host the mud snail Heleobia australis
- Dog’s genotype of Giardia duodenalis in human: first evidence in Europe