Cryptosporidium identification in HIV-Infected Humans. Experience from Kinshasa, the Democratic Republic of Congo
-
Roger D. Wumba
,
Josué Zanga
Abstract
Cryptosporidium is an important protozoan parasite affecting HIV/AIDS patients. To determine the prevalence and the species of Cryptosporidium spp by developing a rapid and efficient real-time PCR-RFLP test. A cross-sectional study was conducted among HIV-infected adults from Kinshasa, the Democratic Republic of Congo. Stool specimens were examined by microscopic evaluation and real-time PCR-RFLP. Out of 242 HIV-infected adults, 10 (4.1%) cases of Cryptosporidium were identified by microscopic examination. Using PCR-RFLP, the prevalence of Cryptosporidium spp was 5.4% (n = 13). All the 13 cases of Cryptosporidium spp had the stage of AIDS of HIV infection. Extracted DNA was amplified by nested PCR targeting a 1030-bp fragment of the 18s RNA gene. RFLP analysis identified one C. parvum, four C. hominis and one non determined Cryptosporidium. The capacity to detect C. parvum, C. hominis and non-determined Cryptosporidium was present among our HIV-infected patients.
References
Beaugerie L., Carbonnel F., Carrat F., Rached A.A., Maslo C., Gendre JP. 1998.Factors of weight loss in patients with HIV and chronic diarrhea. Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, 19, 34-3910.1097/00042560-199809010-00005Search in Google Scholar
Belhadj S., Kallel K., Boussen N., Ghobantini A., Bejaoui M., Ben Salem N., Zribi A., Ben Chaabane T., Chaker E. 1999. Role of cryptosporidia and microsporidia in diarrhea in immunocompromised patients. Tunis Med, 77, 638-643Search in Google Scholar
Casemore D.P., Amstrong M., Jackson B.1984. Screening for Cryptosporidium in stools. Lancet, 1,734-73510.1016/S0140-6736(84)92245-1Search in Google Scholar
Cordell R.L., Thor P.M., Addiss D.G., Theurer J., Lichterman R., Ziliak S.R.1997. Impact of a massive waterborne cryptosporidiosis outbreak on child care facilities in metropolitan Milwaukee, Wisconsin. The Pediatric Infectious Disease Journal, 16, 639-64410.1097/00006454-199707000-00003Search in Google Scholar
Corso P.S., Kramer M.H., Blair K.A., Addiss D.G., Davis J.P., Haddix A.C.2003. Cost of illness in the 1993 waterborne Cryptosporidium outbreak, Milwaukee, Wisconsin. Emerging Infectious Diseases, 9, 426-43110.3201/eid0904.020417Search in Google Scholar
Coupe S., Sarfati C., Hamane S., Derouin F. 2005. Detection of cryptosporidium and identification to the species level by nested PCR and restriction fragment length polymorphism. Journal of Clinical Microbiology, 43, 1017-102310.1128/JCM.43.3.1017-1023.2005Search in Google Scholar
Democratic Republic of Congo/Ministry of Planning/ Demographic and health survey 2007. Kinshasa, 2007Search in Google Scholar
Dieng T., Ndir O., Diallo S., Coll-Seck A.M., Dieng Y.1994. Prevalence of Cryptosporidium sp and Isospora belli in patients with acquired immunodeficiency syndrome (AIDS) in Dakar (Senegal]. Dakar Med, 39, 121-124Search in Google Scholar
Essid R., Mousli M., Aoun K., Abdelmalek R., Mellouli F., Kanoun F. , Derouin F., Bouratbine A.2008. Identification of cryptosporidium species infecting humans in Tunisia. American Journal of Tropical Medicine and Hygiene, 79,702-70510.4269/ajtmh.2008.79.702Search in Google Scholar
Fontaine M., Guillot E. 2003. Study of 18S rRNA and rDNA stability by real-time RT-PCR in heat-inactivated Cryptosporidium parvum oocysts. FEMS Microbiology Letters, 226, 237-24310.1016/S0378-1097(03)00538-XSearch in Google Scholar
Gatei W., Wamae C.N., Mbae C., Waruru A., Mulinge E., Waithera T., Gatika S.M., Kamwati S.K., Revathi G., Hart C.A. 2006. Cryptosporidiosis: prevalence, genotype analysis, and symptoms associated with infections in children in Kenya. American Journal of Tropical Medicine and Hygiene, 75, 78-8210.4269/ajtmh.2006.75.78Search in Google Scholar
Guitard J., Menotti J., Desveaux A., Alimardani P., Porcher R., Derouin F., Kapel N. 2006. Experimental study of the effects of probiotics on Cryptosporidium parvum infection in neonatal rats. Parasitology Research, 99, 522-52710.1007/s00436-006-0181-4Search in Google Scholar
Khumalo-Ngwenya B., Luo N.P., Chintu C., Sunkutu R., Sakala- Kazembe F., Baboo K.S., Mathewson J., Zumla A. 1994. Gut parasites in HIV-seropositive Zambian adults with diarrhoea. East African Medical Journal, 71, 379-383Search in Google Scholar
Leitch G.J., He Q. 2012. Cryptosporidiosis-an overview. Journal of Biomedical Research, 25, 1-1610.1016/S1674-8301(11)60001-8Search in Google Scholar
Mengesha B. 1994.Cryptosporidiosis among medical patients with the acquired immunodeficiency syndrome in Tikur Anbessa Teaching Hospital, Ethiopia. East African Medical Journal, 71, 376-378Search in Google Scholar
Menotti J., Cassinat B., Porcher R., Sarfati C., Derouin F., Molina J.M. 2003. Development of a real-time polymerase-chainreaction assay for quantitative detection of Enterocytozoon bieneusi DNA in stool specimens from immunocompromised patients with intestinal microsporidiosis. Journal of Infectious Diseases, 187, 1469-147410.1086/374620Search in Google Scholar
Miao Y.M., Awad-El-Kariem F.M., Franzen C., Ellis D.S., Muller A., Counihan H.M., Hayes P.J., Gazzard B.G. 2000. Eradication of cryptosporidia and microsporidia following successful antiretroviral therapy. Journal of Acquired Immune Deficiency Syndromes, 25, 124-12910.1097/00126334-200010010-00006Search in Google Scholar
Mwachari C., Batchelor B.I., Paul J., Waiyaki P.G., Gilks C.F. 1998. Chronic diarrhoea among HIV-infected adult patients in Nairobi, Kenya. Journal of Infection, 37, 48-5310.1016/S0163-4453(98)90561-8Search in Google Scholar
UNAIDS. Report of the global AIDS epidemic, 2008. Geneva. Joint United Nations Program on HIV/AIDS, 2008Search in Google Scholar
World Health Organization. Global HIV/AIDS Response Epidemic update and health sector progress towards Universal Access. Progress Report 2011. Geneva, 2011Search in Google Scholar
Wumba R., Enache-Angoulvant A., Develoux M., Mulumba A., Mulumba P.M., Hennequin C., Odio T.W., Biligui S., Sala J., Thellier M. 2007. Prevalence of opportunistic digestive parasitic infections in Kinshasa, Democratic Republic of Congo. Results of a preliminary study in 50 AIDS patients. Med Trop (Mars), 67, 145-148Search in Google Scholar
Wumba R., Longo-Mbenza B., Mandina M., Odio W.T., Biligui S., Sala J., Breton J., Thellier M.2010. Intestinal parasites infections in hospitalized AIDS patients in Kinshasa, Democratic Republic of the Congo. Parasite, 17, 321-32810.1051/parasite/2010174321Search in Google Scholar PubMed
Wumba R., Longo-Mbenza B., Menotti J., Mandina M., Kintoki F., Situakibanza N.H., Kakicha M.K., Zanga J., Mbanzulu- Makola K., Nseka T., Mukendi J.P., Kendjo E., Sala J., Thellier M. 2012. Epidemiology, clinical, immune, and molecular profiles of microsporidiosis and cryptosporidiosis among HIV/AIDS patients. International Journal of General Medicine, 5, 603-611. DOI: 10.2147/IJGM.S32344 10.2147/IJGM.S32344Search in Google Scholar PubMed PubMed Central
W. Stefański Institute of Parasitology, PAS
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- A survey for potentially zoonotic gastrointestinal parasites of dogs and pigs in Cambodia
- Tritrichomonas foetus infection in cat – first detection in Poland
- Association between immunoreactivity to Anisakis spp. antigens and high-risk pregnancy
- Stibarokris mariasi sp. nov. – a new quill mite species (Acariformes: Syringophilidae) parasitizing Puffinus pacificus (Gmelin) (Procellariidae) on Johnston Atoll
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- Molecular characterization of cystic echinococcosis: First record of G7 in Egypt and G1 in Yemen
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- Morphologic and molecular characteristics of Sarcocystis atraii n. sp. (Apicomplexa: Sarcocystidae) infecting the common coot (Fulica atra) from Egypt
- Auto-infection by Echinostoma spp. cercariae in Helisoma anceps
- Risk factors of occurrence of Toxoplasma gondii among horses in the state of Alagoas, Brazil
- Cardiopulmonary helminths in foxes from the Pyrenees
- Risk of soil-transmitted helminth infections on agritourism farms in central and eastern Poland
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